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Volumn 10, Issue 2, 2006, Pages 162-168

High-throughput screening of biocatalytic activity: Applications in drug discovery

Author keywords

[No Author keywords available]

Indexed keywords

CYTOCHROME P450; LEAD; PHOSPHOTRANSFERASE INHIBITOR;

EID: 33645218742     PISSN: 13675931     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cbpa.2006.02.033     Document Type: Review
Times cited : (48)

References (47)
  • 1
    • 1642354534 scopus 로고    scopus 로고
    • Surviving the blockbuster syndrome
    • R.F. Service Surviving the blockbuster syndrome Science 303 2004 1796 1799
    • (2004) Science , vol.303 , pp. 1796-1799
    • Service, R.F.1
  • 3
    • 1642323740 scopus 로고    scopus 로고
    • Protein kinase inhibitors: Insights into drug design from structure
    • M.E.M. Noble, J.A. Endicott, and L.N. Johnson Protein kinase inhibitors: insights into drug design from structure Science 303 2004 1800 1805
    • (2004) Science , vol.303 , pp. 1800-1805
    • Noble, M.E.M.1    Endicott, J.A.2    Johnson, L.N.3
  • 4
    • 15244353524 scopus 로고    scopus 로고
    • Exploring the potential of xenobiotic-metabolizing enzymes as biocatalysts: Evolving designer catalysts from polyfunctional cytochrome P450 enzymes
    • E.M.J. Gillam Exploring the potential of xenobiotic-metabolizing enzymes as biocatalysts: evolving designer catalysts from polyfunctional cytochrome P450 enzymes Clin Exp Pharm Physiol 32 2005 147 152
    • (2005) Clin Exp Pharm Physiol , vol.32 , pp. 147-152
    • Gillam, E.M.J.1
  • 5
    • 7744246367 scopus 로고    scopus 로고
    • Life's smile, death's grin: Vital functions of apoptosis-executing proteins
    • C. Garrido, and G. Kroemer Life's smile, death's grin: vital functions of apoptosis-executing proteins Curr Opin Cell Biol 16 2004 639 646
    • (2004) Curr Opin Cell Biol , vol.16 , pp. 639-646
    • Garrido, C.1    Kroemer, G.2
  • 6
    • 0037362544 scopus 로고    scopus 로고
    • Combinatorial compound libraries for drug discovery: An ongoing challenge
    • H.M. Geysen, F. Schoenen, D. Wagner, and R. Wagner Combinatorial compound libraries for drug discovery: an ongoing challenge Nat Rev Drug Discov 2 2003 222 230
    • (2003) Nat Rev Drug Discov , vol.2 , pp. 222-230
    • Geysen, H.M.1    Schoenen, F.2    Wagner, D.3    Wagner, R.4
  • 10
    • 20444432725 scopus 로고    scopus 로고
    • High-throughput screening for kinase inhibitors
    • O. von Ahsen, and U. Bomer High-throughput screening for kinase inhibitors ChemBioChem 6 2005 481 490
    • (2005) ChemBioChem , vol.6 , pp. 481-490
    • Von Ahsen, O.1    Bomer, U.2
  • 11
    • 14744271309 scopus 로고    scopus 로고
    • How to choose an in vitro kinase assay
    • A. Shah How to choose an in vitro kinase assay Drug Discov Dev 7 2004 59 62
    • (2004) Drug Discov Dev , vol.7 , pp. 59-62
    • Shah, A.1
  • 12
    • 0842332512 scopus 로고    scopus 로고
    • High-throughput screening for stability and inhibitory activity of compounds toward cytochrome P450-mediated metabolism
    • J.H. Ansede, and D.R. Thakker High-throughput screening for stability and inhibitory activity of compounds toward cytochrome P450-mediated metabolism J Pharm Sci 93 2004 239 255
    • (2004) J Pharm Sci , vol.93 , pp. 239-255
    • Ansede, J.H.1    Thakker, D.R.2
  • 14
    • 2942525302 scopus 로고    scopus 로고
    • Applications of high-throughput ADME in drug discovery
    • D.B. Kassel Applications of high-throughput ADME in drug discovery Curr Opin Chem Biol 8 2004 339 345
    • (2004) Curr Opin Chem Biol , vol.8 , pp. 339-345
    • Kassel, D.B.1
  • 16
    • 0037140423 scopus 로고    scopus 로고
    • Sol-gel encapsulated enzyme arrays for high-throughput screening of biocatalytic activity
    • C.B. Park, and D.S. Clark Sol-gel encapsulated enzyme arrays for high-throughput screening of biocatalytic activity Biotechnol Bioeng 78 2002 229 235 Enzymes encapsulated in sol-gels, referred to as solzymes, are used in a low-cost and reusable multi-well platform for high-throughput biocatalytic assays.
    • (2002) Biotechnol Bioeng , vol.78 , pp. 229-235
    • Park, C.B.1    Clark, D.S.2
  • 17
    • 29244470287 scopus 로고    scopus 로고
    • Nanovolume kinase inhibition assay using a sol-gel-derived multicomponent microarray
    • N. Rupcich, J.R. Green, and J.D. Brennan Nanovolume kinase inhibition assay using a sol-gel-derived multicomponent microarray Anal Chem 77 2005 8013 8019
    • (2005) Anal Chem , vol.77 , pp. 8013-8019
    • Rupcich, N.1    Green, J.R.2    Brennan, J.D.3
  • 18
    • 1842675214 scopus 로고    scopus 로고
    • Putting thought to paper: A μaRCS protease screen
    • D.R. Groebe, M.L. Maus, T. Pederson, J. Clampit, S. Djuric, J. Trevillyan, C.W. Lin, D.J. Burns, and U. Warrior Putting thought to paper: a μARCS protease screen J Biomol Screen 8 2003 668 675 A high-throughput platform called μARCS was used to screen for protease inhibitors. Enzyme-encapsulated agarose gel was contacted with a sheet of arrayed compounds and a substrate gel/sheet. The gel matrix acts as a reaction vessel and the inhibitors are identified using fluorescent probes.
    • (2003) J Biomol Screen , vol.8 , pp. 668-675
    • Groebe, D.R.1    Maus, M.L.2    Pederson, T.3    Clampit, J.4    Djuric, S.5    Trevillyan, J.6    Lin, C.W.7    Burns, D.J.8    Warrior, U.9
  • 20
    • 12244253039 scopus 로고    scopus 로고
    • A high-throughput, low-volume enzyme assay on solid support
    • P. Babiak, and J.-L. Reymond A high-throughput, low-volume enzyme assay on solid support Anal Chem 77 2005 373 377
    • (2005) Anal Chem , vol.77 , pp. 373-377
    • Babiak, P.1    Reymond, J.-L.2
  • 21
    • 0034622925 scopus 로고    scopus 로고
    • Printing proteins as microarrays for high-throughput function determination
    • G. MacBeath, and S.L. Schreiber Printing proteins as microarrays for high-throughput function determination Science 289 2000 1760 1763
    • (2000) Science , vol.289 , pp. 1760-1763
    • MacBeath, G.1    Schreiber, S.L.2
  • 23
    • 0242432396 scopus 로고    scopus 로고
    • Developing a strategy for activity-based detection of enzymes in a protein microarray
    • G.Y.J. Chen, M. Uttamchandani, Q. Zhu, G. Wang, and S.Q. Yao Developing a strategy for activity-based detection of enzymes in a protein microarray ChemBioChem 4 2003 336 339 The first demonstration of direct enzyme immobilization on glass slides for high-throughput activity assays and screening of enzyme inhibitors. Mechanism-based fluorescent probes were used to identify inhibitors of cysteine proteases, serine hydrolases, and phosphatases.
    • (2003) ChemBioChem , vol.4 , pp. 336-339
    • Chen, G.Y.J.1    Uttamchandani, M.2    Zhu, Q.3    Wang, G.4    Yao, S.Q.5
  • 24
    • 4444290143 scopus 로고    scopus 로고
    • Enzyme microarrays:On-chip determination of inhibition constants based on affinity-label detection of enzymatic activity
    • J. Eppinger, D.P. Funeriu, M. Miyake, L. Denizot, and J. Miyake Enzyme microarrays:On-chip determination of inhibition constants based on affinity-label detection of enzymatic activity Angew Chem Int Ed Engl 43 2004 3806 3810 An immobilized protein microarray was used in conjunction with activity-dependent fluorescent labels to characterize the kinetics of cysteine proteases and the mechanism of protease inhibitors.
    • (2004) Angew Chem Int Ed Engl , vol.43 , pp. 3806-3810
    • Eppinger, J.1    Funeriu, D.P.2    Miyake, M.3    Denizot, L.4    Miyake, J.5
  • 26
    • 4043158549 scopus 로고    scopus 로고
    • Profiling of generic anti-phosphopeptide antibodies and kinases with peptide microarrays using radioactive and fluorescence-based assays
    • S. Panse, L. Dong, A. Burian, R. Carus, M. Schutkowski, U. Reimer, and J. Schneider-Mergener Profiling of generic anti-phosphopeptide antibodies and kinases with peptide microarrays using radioactive and fluorescence-based assays Mol Divers 8 2004 291 299
    • (2004) Mol Divers , vol.8 , pp. 291-299
    • Panse, S.1    Dong, L.2    Burian, A.3    Carus, R.4    Schutkowski, M.5    Reimer, U.6    Schneider-Mergener, J.7
  • 27
    • 0037132625 scopus 로고    scopus 로고
    • Peptide microarrays for the determination of protease substrate specificity
    • C.M. Salisbury, D.J. Maly, and J.A. Ellman Peptide microarrays for the determination of protease substrate specificity J Am Chem Soc 124 2002 14868 14870
    • (2002) J Am Chem Soc , vol.124 , pp. 14868-14870
    • Salisbury, C.M.1    Maly, D.J.2    Ellman, J.A.3
  • 29
    • 32644445255 scopus 로고    scopus 로고
    • Combinatorial solid phase peptide synthesis and bioassays
    • D.-S. Shin, D.-H. Kim, W.-J. Chung, and Y.-S. Lee Combinatorial solid phase peptide synthesis and bioassays J Biochem Mol Biol 38 2005 517 525
    • (2005) J Biochem Mol Biol , vol.38 , pp. 517-525
    • Shin, D.-S.1    Kim, D.-H.2    Chung, W.-J.3    Lee, Y.-S.4
  • 31
    • 0041806643 scopus 로고    scopus 로고
    • Printing chemical libraries on microarrays for fluid phase nanoliter reactions
    • D.N. Gosalia, and S.L. Diamond Printing chemical libraries on microarrays for fluid phase nanoliter reactions Proc Natl Acad Sci USA 100 2003 8721 8726 A novel approach to biocatalytic assays on a microarray without the need for physical or covalent immobilization of enzymes. Nanoliter volume reactions were used to screen inhibitors against caspases, thrombin and chymotrypsin.
    • (2003) Proc Natl Acad Sci USA , vol.100 , pp. 8721-8726
    • Gosalia, D.N.1    Diamond, S.L.2
  • 32
    • 17044388248 scopus 로고    scopus 로고
    • Profiling serine protease substrate specificity with solution phase fluorogenic peptide microarrays
    • D.N. Gosalia, C.M. Salisbury, D.J. Maly, J.A. Ellman, and S.L. Diamond Profiling serine protease substrate specificity with solution phase fluorogenic peptide microarrays Proteomics 5 2005 1292 1298
    • (2005) Proteomics , vol.5 , pp. 1292-1298
    • Gosalia, D.N.1    Salisbury, C.M.2    Maly, D.J.3    Ellman, J.A.4    Diamond, S.L.5
  • 34
    • 1642294294 scopus 로고    scopus 로고
    • Organic chemistry in drug discovery
    • M. MacCoss, and T.A. Baillie Organic chemistry in drug discovery Science 303 2004 1810 1813
    • (2004) Science , vol.303 , pp. 1810-1813
    • MacCoss, M.1    Baillie, T.A.2
  • 35
    • 27644596457 scopus 로고    scopus 로고
    • Predicting in vivo drug interactions from in vitro drug discovery data
    • L.C. Wienkers, and T.G. Heath Predicting in vivo drug interactions from in vitro drug discovery data Nat Rev Drug Discov 4 2005 825 833
    • (2005) Nat Rev Drug Discov , vol.4 , pp. 825-833
    • Wienkers, L.C.1    Heath, T.G.2
  • 36
    • 17044381648 scopus 로고    scopus 로고
    • Highly miniaturized formats for in vitro drug metabolism assays using Vivid fluorescent substrates and recombinant human cytochrome P450 enzymes
    • O.V. Trubetskoy, J.R. Gibson, and B.D. Marks Highly miniaturized formats for in vitro drug metabolism assays using Vivid fluorescent substrates and recombinant human cytochrome P450 enzymes J Biomol Screen 10 2005 56 66
    • (2005) J Biomol Screen , vol.10 , pp. 56-66
    • Trubetskoy, O.V.1    Gibson, J.R.2    Marks, B.D.3
  • 37
    • 27744435930 scopus 로고    scopus 로고
    • Maximizing automation in LC/MS high-throughput analysis and purification
    • M. Koppitz, A. Brailsford, and M. Wenz Maximizing automation in LC/MS high-throughput analysis and purification J Comb Chem 7 2005 714 720
    • (2005) J Comb Chem , vol.7 , pp. 714-720
    • Koppitz, M.1    Brailsford, A.2    Wenz, M.3
  • 38
    • 0037232358 scopus 로고    scopus 로고
    • High-throughput cytochrome P450 inhibition assays by ultrafast gradient liquid chromatography with tandem mass spectrometry using monolithic columns
    • S.X. Peng, A.G. Barbone, and D.M. Ritchie High-throughput cytochrome P450 inhibition assays by ultrafast gradient liquid chromatography with tandem mass spectrometry using monolithic columns Rapid Commun Mass Spectrom 17 2003 509 518 A significant increase in the throughput of LC/MS by sample pooling techniques and ultrafast chromatography.
    • (2003) Rapid Commun Mass Spectrom , vol.17 , pp. 509-518
    • Peng, S.X.1    Barbone, A.G.2    Ritchie, D.M.3
  • 39
    • 0036112196 scopus 로고    scopus 로고
    • Application of parallel liquid chromatography/mass spectrometry for high throughput microsomal stability screening of compound libraries
    • R. Xu, C. Nemes, K.M. Jenkins, R.A. Rourick, and D.B. Kassel Application of parallel liquid chromatography/mass spectrometry for high throughput microsomal stability screening of compound libraries J Am Soc Mass Spectrom 13 2002 155 165
    • (2002) J Am Soc Mass Spectrom , vol.13 , pp. 155-165
    • Xu, R.1    Nemes, C.2    Jenkins, K.M.3    Rourick, R.A.4    Kassel, D.B.5
  • 41
    • 12844253069 scopus 로고    scopus 로고
    • Metabolizing enzyme toxicology assay chip (MetaChip) for high-throughput microscale toxicity analyses
    • M.-Y. Lee, C.B. Park, J.S. Dordick, and D.S. Clark Metabolizing enzyme toxicology assay chip (MetaChip) for high-throughput microscale toxicity analyses Proc Natl Acad Sci USA 102 2005 983 987 A powerful approach for rapid screening of drugs and drug candidates for cytotoxicity, using nanoliter volume sol-gel encapsulated CYPs on a glass slide for the generation of metabolites. The cytotoxicity profiles obtained from the microarray are comparable to those obtained by conventional well-plate assays.
    • (2005) Proc Natl Acad Sci USA , vol.102 , pp. 983-987
    • Lee, M.-Y.1    Park, C.B.2    Dordick, J.S.3    Clark, D.S.4
  • 42
    • 7044274425 scopus 로고    scopus 로고
    • A homogeneous, nonradioactive high-throughput fluorogenic protein phosphatase assay
    • K. Kupcho, K. Hsiao, B. Bulleit, and S.A. Goueli A homogeneous, nonradioactive high-throughput fluorogenic protein phosphatase assay J Biomol Screen 9 2004 223 231
    • (2004) J Biomol Screen , vol.9 , pp. 223-231
    • Kupcho, K.1    Hsiao, K.2    Bulleit, B.3    Goueli, S.A.4
  • 44
    • 2442583662 scopus 로고    scopus 로고
    • High-throughput screening with immobilized metal ion affinity-based fluorescence polarization detection, a homogeneous assay for protein kinases
    • E.E.M.G. Loomans, A.M. van Doornmalen, J.W.Y. Wat, and G.J.R. Zaman High-throughput screening with immobilized metal ion affinity-based fluorescence polarization detection, a homogeneous assay for protein kinases Assay Drug Dev Technol 1 2003 445 453
    • (2003) Assay Drug Dev Technol , vol.1 , pp. 445-453
    • Loomans, E.E.M.G.1    Van Doornmalen, A.M.2    Wat, J.W.Y.3    Zaman, G.J.R.4
  • 45
    • 2442482366 scopus 로고    scopus 로고
    • Miniaturized, ultra-high throughput screening of tyrosine kinases using homogeneous, competitive fluorescence immunoassays
    • J.R. Beasley, P.M. McCoy, T.L. Walker, and D.A. Dunn Miniaturized, ultra-high throughput screening of tyrosine kinases using homogeneous, competitive fluorescence immunoassays Assay Drug Dev Techol 2 2004 141 151
    • (2004) Assay Drug Dev Techol , vol.2 , pp. 141-151
    • Beasley, J.R.1    McCoy, P.M.2    Walker, T.L.3    Dunn, D.A.4
  • 46
    • 16544373302 scopus 로고    scopus 로고
    • High-throughput cell-based screening using scintillation proximity assay for the discovery of inositol phosphatase inhibitors
    • W. Zheng, P.E. Brandish, D.G. Kolodin, E.M. Scolnick, and B. Strulovict High-throughput cell-based screening using scintillation proximity assay for the discovery of inositol phosphatase inhibitors J Biomol Screen 9 2004 132 140
    • (2004) J Biomol Screen , vol.9 , pp. 132-140
    • Zheng, W.1    Brandish, P.E.2    Kolodin, D.G.3    Scolnick, E.M.4    Strulovict, B.5
  • 47
    • 2442457423 scopus 로고    scopus 로고
    • High-throughput screening with quantitation of ATP consumption: A universal non-radioisotope, homogeneous assay for protein kinase
    • M. Koresawa, and T. Okabe High-throughput screening with quantitation of ATP consumption: a universal non-radioisotope, homogeneous assay for protein kinase Assay Drug Dev Techol 2 2004 153 160
    • (2004) Assay Drug Dev Techol , vol.2 , pp. 153-160
    • Koresawa, M.1    Okabe, T.2


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