Putting thought to paper: A μARCS protease screen

Journal of Biomolecular Screening

Volumn 8, Issue 6, 2003, Pages 668-675

  Groebe, Duncan R ^a,b   Maus, Mary L ^a   Pederson, Terry ^a   Clampit, Jill ^a   Djuric, Stevan ^a   Trevillyan, James ^a   Lin, Chun W ^a   Burns, David J ^a   Warrior, Usha ^a  

^a ABBOTT LABORATORIES   (United States)

^b R4PN/J35 ^*  (United States)

Author keywords

ARCS; High throughput screening; HTS

Indexed keywords

AGAROSE; PROTEINASE INHIBITOR; REAGENT; DIMETHYL SULFOXIDE; PROTEINASE; SEPHAROSE;

ARTICLE; CONTROLLED STUDY; COST; DIFFUSION; DRUG DETERMINATION; DRUG TARGETING; ENZYME MECHANISM; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; HUMIDITY; IC 50; MICROARRAYED COMPOUND SCREENING; PRIORITY JOURNAL; PROTEIN TARGETING; CELL STRAIN CACO 2; ENZYME SPECIFICITY; METABOLISM; METHODOLOGY; PROTEIN MICROARRAY;

CACO-2 CELLS; DIMETHYL SULFOXIDE; ENDOPEPTIDASES; HUMANS; HUMIDITY; INHIBITORY CONCENTRATION 50; PROTEASE INHIBITORS; PROTEIN ARRAY ANALYSIS; SEPHAROSE; SUBSTRATE SPECIFICITY;

EID: 1842675214     PISSN: 10870571     EISSN: None     Source Type: Journal    
DOI: 10.1177/1087057103258587     Document Type: Article

References (14)

1. Ouchterlony O: Diffusion-in-gel methods for immunological analysis. Prog Allergy 1958;5:1-18.
2. Davis WW, Stout TT: Disc plate method of microbiological antibiotic assay. II. Novel procedure offering improved accuracy. Appl Microbiol 1971;4:666-670.
3. Bell SM: The calibrated dichotomous sensitivity (CDS) disc method of antibiotic sensitivity testing. Pathology 1975;7(suppl):1-48.
4. Jayawickreme CK, Graminski GF, Quillan JM: Creation and functional screening of a multi-use peptide library. Proc Natl Acad Sci U S A 1994;91:1614-1618.
5. Quillan JM, Jayawickreme CK, Lermer MR: Combinatorial diffusion assay used to identify topically active melanocyte-stimulating hormone receptor antagonists. Proc Natl Acad Sci U S A 1994;92:2894-2898.
6. Salmon SE, Liu-Stevens RH, Zhao Y, et al: High-volume cellular screening for anticancer agents with combinatorial chemical libraries: a new methodology. Mol Divers 1996;2:57-63.
7. Burns DJ, Kofron JL, Warrior U, Beutel BA: Well-less, gel-permation formats for ultra-HTS. Drug Discov Today 2001;(suppl 6):S144-S151.
8. David CA, Middleton T, Montgonery D, et al: Microarray compound screening (μARCS) to identify inhibitors of HIV integrase. J Biomol Screen 2002;7:259-266.
9. Gopalakrishnan SM, Karvinen J, Kofron JL, Burns DJ, Warrior U: Application of micro-arrayed compound screening (μARCS) to identify inhibitors of caspase-3. J Biomol Screen 2002;7:317-323.
10. Xuei X, David CA, Middleton TR, et al: Use of SAM™ Biotin Capture Membranes in microarrayed compound screening (μARCS) format for nucleic acid polymerization assays. J Biomol Screen 2003;8:273-282.
11. Humphrey P, Gonzalez C, Jones R, et al: Nanoliter dispensing of compound libraries for use in continuous format high-throughput screening. Paper presented at the 5th annual conference and exhibition of the Society for Biomolecular Screening, Edinburgh, UK, September 1999.
12. Vorrbach J, Wakat J, Kidonakis I, et al: Custom software for use in continuous format high-throughput screening. Paper presented at the 5th annual conference and exhibition of the Society for Biomolecular Screening, Edinburgh, UK, September 1999.
13. Fan F, Anderson S, Wang J, et al: Application of microarrayed compound screening (μARCS) to identify inhibitors of methionine aminopeptidase type 2. Poster presented at the 8th annual conference and exhibition of the Society for Biomolecular Screening, The Hague, the Netherlands, September 2002.
14. Groebe DR, Maus M, Sabet R, Warrior U, Burns D: On beyond ultra-HTS. Paper presented at the 8th annual conference and exhibition of the Society for Biomolecular Screening, The Hague, the Netherlands, September 2002.