Using an AraC-based three-hybrid system to detect biocatalysts in vivo

Nature Biotechnology

Volumn 18, Issue 5, 2000, Pages 544-547

  Firestine, Steven M ^a   Salinas, Frank ^a   Nixon, Andrew E ^b   Baker, Stephen J ^a   Benkovic, Stephen J ^a  

^a PENNSYLVANIA STATE UNIVERSITY   (United States)

^b Dyax Corp   (United States)

Author keywords

Artificial transcription system; Molecular evolution; Screening selection methods; Scytalone dehydratase; Three hybrid systems

Indexed keywords

ARABINOSE; CYTARABINE; FUNGAL ENZYME; HYDROLYASE;

ARTICLE; BIOTECHNOLOGY; CATALYST; ENZYME MECHANISM; ENZYME SUBSTRATE; GENE ACTIVATION; GENE CONSTRUCT; GENE EXPRESSION SYSTEM; GENE LIBRARY; GENETIC SCREENING; HYBRID; MOLECULAR EVOLUTION; NONHUMAN; OPERON; PRIORITY JOURNAL; REPORTER GENE; TRANSCRIPTION REGULATION;

ARAC TRANSCRIPTION FACTOR; BACTERIAL PROTEINS; BENZOPYRANS; CATALYSIS; CLONING, MOLECULAR; DIMERIZATION; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FUNGAL PROTEINS; GENES, REPORTER; HYDRO-LYASES; MOLECULAR PROBE TECHNIQUES; PROTEIN BINDING; REPRESSOR PROTEINS; SELECTION (GENETICS); SENSITIVITY AND SPECIFICITY; TRANSCRIPTION FACTORS; TRANSCRIPTION, GENETIC;

BACTERIA (MICROORGANISMS);

EID: 0034022841     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/75414     Document Type: Article

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