Structures of the bacterial ribosome at 3.5 Å resolution

Science

Volumn 310, Issue 5749, 2005, Pages 827-834

  Schuwirth, Barbara S ^a,c   Borovinskaya, Maria A ^b   Hau, Cathy W ^a   Zhang, Wen ^a   Vila Sanjurjo, Antón ^b   Holton, James M ^b   Cate, Jamie H Doudna ^a,b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b LAWRENCE BERKELEY NATIONAL LABORATORY   (United States)

^c FREIE UNIVERSITÄT BERLIN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CELL CULTURE; CYTOLOGY; ESCHERICHIA COLI; OPTICAL RESOLVING POWER; RNA; X RAY CRYSTALLOGRAPHY;

MESSENGER RNA; RIBOSOMAL SUBUNITS; RIBOSOMES; TRANSLOCATION;

BACTERIOLOGY;

MESSENGER RNA; PEPTIDYLTRANSFERASE; TRANSFER RNA;

BIOLOGY;

ARTICLE; ENZYME CONFORMATION; ESCHERICHIA COLI; MOLECULAR DYNAMICS; NONHUMAN; OPTICAL RESOLUTION; PRIORITY JOURNAL; RIBOSOME; RIBOSOME SUBUNIT; RNA STRUCTURE; X RAY CRYSTALLOGRAPHY;

BINDING SITES; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; HYDROGEN BONDING; MAGNESIUM; MODELS, MOLECULAR; NUCLEIC ACID CONFORMATION; PEPTIDYL TRANSFERASES; PROTEIN BIOSYNTHESIS; PROTEIN CONFORMATION; RIBOSOMAL PROTEINS; RIBOSOMES; RNA, BACTERIAL; RNA, MESSENGER; RNA, RIBOSOMAL; RNA, TRANSFER;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 27644491082     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1117230     Document Type: Article

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74. We thank J. Plecs, K. Frankel, G. Hura, and G. Meigs for help with data measurement at the SIBYLS and 8.3.1 beamlines at the Advanced Light Source; J. Frank for providing the cryo-EM reconstructions of the E. coli 70S ribosome; W. Ridgeway for rapid crystallization screen designs; A. Borovinskiy for help with protein modeling; and J. Doudna, P. Adams, V. Ramakrishnan, H. Noller, and R. Green for helpful discussions and comments on the manuscript. A.V.-S. acknowledges F. Vila for balance and coherence. Atomic coordinates and structure factors are deposited in the Protein Data Bank (accession codes 2AVY, 2AW4, 2AW7, and 2AWB). Supported by NIH grant GM65050 (J.H.D.C.), National Cancer Institute grant CA92S84 (for the SIBYLS and B.3.1 beamlines), and U.S. Department of Energy grants DE-AC03-76SF00098, KP110201, and LBNL LORD 366851 (J.H.D.C.) and DE-AC03 76SF00098 (for the SIBYLS and 83.1 beamlines).