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Volumn 273, Issue 5282, 1996, Pages 1696-1699

RNA tertiary structure mediation by adenosine platforms

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BASE PAIRING; CHEMICAL MODIFICATION; CRYSTAL STRUCTURE; DNA HELIX; INTRON; MOLECULAR INTERACTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN TERTIARY STRUCTURE; RNA STRUCTURE; SEQUENCE HOMOLOGY;

EID: 0029796982     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.273.5282.1696     Document Type: Article
Times cited : (318)

References (29)
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    • note
    • C electron density map is consistent with a metal ion or water molecule in the major groove coordinated to the ribose and phosphate of the 5′ A and 3′ A of the platform, respectively, and to N7 and 06 of the G in the G·U wobble below the motif. In the L5c loop platform, however, there is density for a putative magnesium ion coordinated to phosphates of the 3′ A of the platform and the adjacent A of the A·U noncanonical pair. Both molecules in the asymmetric unit reflect these differences between the platforms.
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    • 1 in side-by-side experiments. Preliminary RNA splicing analysis of a precursor RNA with a two-base change in the third adenosine platform, A171-A172 to U-U, showed a twofold reduction in activity at low magnesium ion concentration (5 mM) and even less of an effect at higher magnesium concentrations. One example each of potential C-U and U-C platforms occurs in the tetraloop receptor motif of group I introns in subclasses IC1 and IC3.
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    • note
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    • in press
    • J. D. Puglisi, R. Tan, B. J. Calnan, A. D. Frankel, J. R. Williamson. Science 257, 76 (1992); F. Aboul-ela, J. Kam, G. Varani, J. Mol. Biol. 253, 313 (1995); Y. Yang, M. Kochoyan, P. Burgstaller, E. Westhof, M. Famulok, Science 272, 1343 (1996); F. Jiang, R. A. Kumar, R. A. Jones, D. J. Patel, Nature 382, 171 (1996); T. Dieckmann, E. Suzuki, G. K. Nakamura, J. Feigon, RNA, in press.
    • RNA
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    • note
    • Mutation of the J5a/6b platform in P4-P6 from A-A to G-A produced a P4-P6 derivative that appeared to fold correctly in solution (5), but the RNA was not tested for activity.
  • 29
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    • note
    • We thank K. Blount, D. Sheehan, and A. Zaug for functional assays; F. Michel, P. Moore, and A. M. Pyle for helpful discussions; and A. Ferré-d'Amaré, S. Strobel, and T. Griffin for review of the manuscript. This work was funded by the Lucille P. Markey Charitable Trust, the Donaghue Medical Research Foundation and NIH grant GM22778-21 (J.A.D.), NIH training grant 5T32GM08283-07 (J.H.C.), American Cancer Society postdoctoral fellowship (B.L.G.), NSF grant MCB-9221307 (C.E.K.), Howard Hughes Medical Institute (T.R.C.), and the Keck Foundation (C.E.K. and T.R.C.); T.R.C. is a Howard Hughes Medical Institute Investigator; J.A.D. is a Lucille P. Markey Scholar in Biomedical Science, a Young Investigator of the Donaghue Medical Research Foundation, a Searle Scholar, and a Beckman Young Investigator.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.