메뉴 건너뛰기




Volumn 45, Issue 1, 2004, Pages 119-125

The establishment and characterization of cell lines stably expressing human Ku80 tagged with enhanced green fluorescent protein

Author keywords

GFP; Heterodimer; Ku70; Ku80; Radiation

Indexed keywords

CELL CULTURE; CELL DEATH; CELL MEMBRANES; FLUORESCENCE; PROTEINS; REPAIR;

EID: 2542575671     PISSN: 04493060     EISSN: None     Source Type: Journal    
DOI: 10.1269/jrr.45.119     Document Type: Article
Times cited : (16)

References (47)
  • 1
    • 0035093737 scopus 로고    scopus 로고
    • DNA double-strand breaks: Signaling, repair and the cancer connection
    • Khanna, K. K. and Jackson, S. P. (2001) DNA double-strand breaks: signaling, repair and the cancer connection. Nat. Genet. 27: 247-254.
    • (2001) Nat. Genet. , vol.27 , pp. 247-254
    • Khanna, K.K.1    Jackson, S.P.2
  • 2
    • 0037365789 scopus 로고    scopus 로고
    • ATM and related protein kinases: Safeguarding genome integrity
    • Shiloh, Y. (2003) ATM and related protein kinases: safeguarding genome integrity. Nat. Rev. Cancer 3: 155-168.
    • (2003) Nat. Rev. Cancer , vol.3 , pp. 155-168
    • Shiloh, Y.1
  • 4
    • 0035833552 scopus 로고    scopus 로고
    • Structure of the Ku heterodimer bound to DNA and its implications for double-strand break repair
    • Walker, J. R., Corpina, R. A. and Goldberg, J. (2001) Structure of the Ku heterodimer bound to DNA and its implications for double-strand break repair. Nature 412: 607-614.
    • (2001) Nature , vol.412 , pp. 607-614
    • Walker, J.R.1    Corpina, R.A.2    Goldberg, J.3
  • 5
    • 0028791446 scopus 로고
    • DNA double-strand break repair and V(D)J recombination: Involvement of DNA-PK
    • Jackson, S. P. and Jeggo, P. A. (1995) DNA double-strand break repair and V(D)J recombination: involvement of DNA-PK. Trends Biochem. Sci. 20: 412-415.
    • (1995) Trends Biochem. Sci. , vol.20 , pp. 412-415
    • Jackson, S.P.1    Jeggo, P.A.2
  • 6
    • 0033006852 scopus 로고    scopus 로고
    • Ku, a DNA repair protein with multiple cellular functions?
    • Featherstone, C. and Jackson, S. P. (1999) Ku, a DNA repair protein with multiple cellular functions? Mutat. Res. 434: 3-15.
    • (1999) Mutat. Res. , vol.434 , pp. 3-15
    • Featherstone, C.1    Jackson, S.P.2
  • 7
    • 0019374013 scopus 로고
    • Characterization of a high molecular weight acidic nuclear protein recognized by autoantibodies in sera from patients with polymyositis-scleroderma overlap
    • Mimori, T., Akizuki, M., Yamagata, H., Inada, S., Yoshida, S. and Homma, M. (1981) Characterization of a high molecular weight acidic nuclear protein recognized by autoantibodies in sera from patients with polymyositis-scleroderma overlap. J. Clin. Invest. 68: 611-620.
    • (1981) J. Clin. Invest. , vol.68 , pp. 611-620
    • Mimori, T.1    Akizuki, M.2    Yamagata, H.3    Inada, S.4    Yoshida, S.5    Homma, M.6
  • 8
    • 0030339852 scopus 로고    scopus 로고
    • The DNA-dependent protein kinase, DNA-PK: 10 Years and no ends in sight
    • Lees-Miller, S. P. (1996) The DNA-dependent protein kinase, DNA-PK: 10 years and no ends in sight. Biochem. Cell Biol. 74: 503-512.
    • (1996) Biochem. Cell Biol. , vol.74 , pp. 503-512
    • Lees-Miller, S.P.1
  • 9
    • 0031214080 scopus 로고    scopus 로고
    • Mammalian DNA double-strand break repair protein XRCC4 interacts with DNA ligase IV
    • Critchlow, S. E., Bowater, R. P. and Jackson, S. P. (1997) Mammalian DNA double-strand break repair protein XRCC4 interacts with DNA ligase IV. Curr. Biol. 7: 588-598.
    • (1997) Curr. Biol. , vol.7 , pp. 588-598
    • Critchlow, S.E.1    Bowater, R.P.2    Jackson, S.P.3
  • 10
    • 0031939411 scopus 로고    scopus 로고
    • The XRCC4 gene product is a target for and interacts with the DNA-dependent protein kinase
    • Leber, R., Wise, T. W., Mizuta, R. and Meek, K. (1998) The XRCC4 gene product is a target for and interacts with the DNA-dependent protein kinase. J. Biol. Chem. 273: 1794-1801.
    • (1998) J. Biol. Chem. , vol.273 , pp. 1794-1801
    • Leber, R.1    Wise, T.W.2    Mizuta, R.3    Meek, K.4
  • 11
    • 0037155703 scopus 로고    scopus 로고
    • Hairpin opening and overhang processing by an Artemis/DNA-dependent protein kinase complex in nonhomologous end joining and V(D)J recombination
    • Ma, Y., Pannicke, U., Schwarz, K. and Lieber, M. R. (2002) Hairpin opening and overhang processing by an Artemis/DNA-dependent protein kinase complex in nonhomologous end joining and V(D)J recombination. Cell 108: 781-794.
    • (2002) Cell , vol.108 , pp. 781-794
    • Ma, Y.1    Pannicke, U.2    Schwarz, K.3    Lieber, M.R.4
  • 14
    • 0032167446 scopus 로고    scopus 로고
    • A targeted DNA-PKcs-null mutation reveals DNA-PK-independent functions for KU in V(D)J recombination
    • Gao, Y., Chaudhuri, J., Zhu, C., Davidson, L., Weaver, D. T. and Alt, F. W. (1998) A targeted DNA-PKcs-null mutation reveals DNA-PK-independent functions for KU in V(D)J recombination. Immunity 9: 367-376.
    • (1998) Immunity , vol.9 , pp. 367-376
    • Gao, Y.1    Chaudhuri, J.2    Zhu, C.3    Davidson, L.4    Weaver, D.T.5    Alt, F.W.6
  • 15
    • 0025028749 scopus 로고
    • Cell surface expression of the 70-kD component of Ku, a DNA-binding nuclear autoantigen
    • Prabhakar, B. S., Allaway, G. P., Srinivasappa, J. and Notkins, A. L. (1990) Cell surface expression of the 70-kD component of Ku, a DNA-binding nuclear autoantigen. J. Clin. Invest. 86: 1301-1305.
    • (1990) J. Clin. Invest. , vol.86 , pp. 1301-1305
    • Prabhakar, B.S.1    Allaway, G.P.2    Srinivasappa, J.3    Notkins, A.L.4
  • 16
    • 0026447440 scopus 로고
    • The nuclear autoimmune antigen Ku is also present on the cell surface
    • Danziel, R. G., Mendelson, S. C. and Quinn, J. P. (1992) The nuclear autoimmune antigen Ku is also present on the cell surface. Autoimmunity 13: 265-267.
    • (1992) Autoimmunity , vol.13 , pp. 265-267
    • Danziel, R.G.1    Mendelson, S.C.2    Quinn, J.P.3
  • 17
    • 0026505959 scopus 로고
    • Antibodies to the p70/p80 (Ku) antigens in systemic lupus erythematosus
    • Reeves, W. H. (1992) Antibodies to the p70/p80 (Ku) antigens in systemic lupus erythematosus. Rheum. Dis. Clin. North. Am. 18: 391-414.
    • (1992) Rheum. Dis. Clin. North. Am. , vol.18 , pp. 391-414
    • Reeves, W.H.1
  • 18
    • 0029916062 scopus 로고    scopus 로고
    • Expression of DNA-dependent protein kinase holoenzyme upon induction of lymphocyte differentiation and V(D)J recombination
    • Grawunder, U., Finnie, N., Jackson, S. P., Riwar, B. and Jessberger, R. (1996) Expression of DNA-dependent protein kinase holoenzyme upon induction of lymphocyte differentiation and V(D)J recombination. Eur. J. Biochem. 241: 931-940.
    • (1996) Eur. J. Biochem. , vol.241 , pp. 931-940
    • Grawunder, U.1    Finnie, N.2    Jackson, S.P.3    Riwar, B.4    Jessberger, R.5
  • 19
    • 0034093748 scopus 로고    scopus 로고
    • Ionizing radiation exposure results in up-regulation of Ku70 via a p53/ataxia-telangiectasia-mutated protein-dependent mechanism
    • Brown, K. D., Lataxes, T. A., Shangary, S., Mannino, J. L., Giardina, J. F., Chen, J. and Baskaran, R. (2000) Ionizing radiation exposure results in up-regulation of Ku70 via a p53/ataxia-telangiectasia-mutated protein-dependent mechanism. J. Biol. Chem. 275: 6651-6656.
    • (2000) J. Biol. Chem. , vol.275 , pp. 6651-6656
    • Brown, K.D.1    Lataxes, T.A.2    Shangary, S.3    Mannino, J.L.4    Giardina, J.F.5    Chen, J.6    Baskaran, R.7
  • 20
    • 0033199987 scopus 로고    scopus 로고
    • Ku in the cytoplasm associates with CD40 in human B cells and translocates into the nucleus following incubation with IL-4 and anti-CD40 mAb
    • Morio, T., Hanissian, S. H., Bacharier, L. B., Teraoka, H., Nonoyama, S., Seki, M., Kondo, J., Nakano, H., Lee, S. K., Geha, R. S. and Yata, J. (1999) Ku in the cytoplasm associates with CD40 in human B cells and translocates into the nucleus following incubation with IL-4 and anti-CD40 mAb. Immunity 11: 339-348.
    • (1999) Immunity , vol.11 , pp. 339-348
    • Morio, T.1    Hanissian, S.H.2    Bacharier, L.B.3    Teraoka, H.4    Nonoyama, S.5    Seki, M.6    Kondo, J.7    Nakano, H.8    Lee, S.K.9    Geha, R.S.10    Yata, J.11
  • 21
    • 0040077747 scopus 로고    scopus 로고
    • Dimerization, translocation and localization of Ku70 and Ku80 proteins
    • Koike, M. (2002) Dimerization, translocation and localization of Ku70 and Ku80 proteins. J. Radiat. Res. 43: 223-236.
    • (2002) J. Radiat. Res. , vol.43 , pp. 223-236
    • Koike, M.1
  • 22
    • 2542535571 scopus 로고    scopus 로고
    • The subcellular localization and the molecular mechanisms of nuclear transport of multifunctional Ku70 and Ku80 Proteins
    • Koike, M. and Koike, A. (2003) The subcellular localization and the molecular mechanisms of nuclear transport of multifunctional Ku70 and Ku80 Proteins. Recent Res. Devel. Biophys. Biochem. 3: 141-158.
    • (2003) Recent Res. Devel. Biophys. Biochem. , vol.3 , pp. 141-158
    • Koike, M.1    Koike, A.2
  • 23
    • 0033539946 scopus 로고    scopus 로고
    • Ku80 can translocate to the nucleus independent of the translocation of Ku70 using its own nuclear localization signal
    • Koike, M., Ikuta, T., Miyasaka, T. and Shiomi, T. (1999) Ku80 can translocate to the nucleus independent of the translocation of Ku70 using its own nuclear localization signal. Oncogene 18: 7495-7505.
    • (1999) Oncogene , vol.18 , pp. 7495-7505
    • Koike, M.1    Ikuta, T.2    Miyasaka, T.3    Shiomi, T.4
  • 24
    • 0033179309 scopus 로고    scopus 로고
    • The nuclear localization signal of the human Ku70 is a variant bipartite type recognized by the two components of nuclear pore-targeting complex
    • Koike, M., Ikuta, T., Miyasaka, T. and Shiomi, T. (1999) The nuclear localization signal of the human Ku70 is a variant bipartite type recognized by the two components of nuclear pore-targeting complex. Exp. Cell Res. 250: 401-413.
    • (1999) Exp. Cell Res. , vol.250 , pp. 401-413
    • Koike, M.1    Ikuta, T.2    Miyasaka, T.3    Shiomi, T.4
  • 25
    • 0034609947 scopus 로고    scopus 로고
    • Ku70 can translocate to the nucleus independent of Ku80 translocation and DNA-PK autophosphorylation
    • Koike, M., Shiomi, T. and Koike, A. (2000) Ku70 can translocate to the nucleus independent of Ku80 translocation and DNA-PK autophosphorylation. Biochem. Biophys. Res. Commun. 276: 1105-1111.
    • (2000) Biochem. Biophys. Res. Commun. , vol.276 , pp. 1105-1111
    • Koike, M.1    Shiomi, T.2    Koike, A.3
  • 26
    • 0035150530 scopus 로고    scopus 로고
    • Nuclear localization of Ku antigen is promoted independently by basic motifs in the Ku70 and Ku80 subunits
    • Bertinato, J., Schild-Poulter, C. and Hache, R. J. (2001) Nuclear localization of Ku antigen is promoted independently by basic motifs in the Ku70 and Ku80 subunits. J. Cell Sci. 114: 89-99.
    • (2001) J. Cell Sci. , vol.114 , pp. 89-99
    • Bertinato, J.1    Schild-Poulter, C.2    Hache, R.J.3
  • 27
    • 0030733163 scopus 로고    scopus 로고
    • Double-strand break repair by Ku70 requires heterodimerization with Ku80 and DNA binding functions
    • Jin, S. and Weaver, D. T. (1997) Double-strand break repair by Ku70 requires heterodimerization with Ku80 and DNA binding functions. EMBO J. 16: 6874-6885.
    • (1997) EMBO J. , vol.16 , pp. 6874-6885
    • Jin, S.1    Weaver, D.T.2
  • 29
    • 0029752779 scopus 로고    scopus 로고
    • Regulation and repair of double-strand DNA breaks
    • Weaver, D. T. (1996) Regulation and repair of double-strand DNA breaks. Crit. Rev. Eukaryot. Gene. Expr. 6: 345-375.
    • (1996) Crit. Rev. Eukaryot. Gene. Expr. , vol.6 , pp. 345-375
    • Weaver, D.T.1
  • 32
    • 0029740571 scopus 로고    scopus 로고
    • Protein-protein and protein-DNA interaction regions within the DNA end-binding protein Ku70-Ku86
    • Wu, X. and Lieber, M. R. (1996) Protein-protein and protein-DNA interaction regions within the DNA end-binding protein Ku70-Ku86. Mol. Cell. Biol. 16: 5186-5193.
    • (1996) Mol. Cell. Biol. , vol.16 , pp. 5186-5193
    • Wu, X.1    Lieber, M.R.2
  • 33
    • 0030827906 scopus 로고    scopus 로고
    • Defining the minimal domain of Ku80 for interaction with Ku70
    • Osipovich, O., Durum, S. K. and Muegge, K. (1997) Defining the minimal domain of Ku80 for interaction with Ku70. J. Biol. Chem. 272: 27259-27265.
    • (1997) J. Biol. Chem. , vol.272 , pp. 27259-27265
    • Osipovich, O.1    Durum, S.K.2    Muegge, K.3
  • 34
    • 0032519378 scopus 로고    scopus 로고
    • A central region of Ku80 mediates interaction with Ku70 in vivo
    • Cary, R. B., Chen, F., Shen, Z. and Chen, D. J. (1998) A central region of Ku80 mediates interaction with Ku70 in vivo. Nucleic Acids Res. 26: 974-979.
    • (1998) Nucleic Acids Res. , vol.26 , pp. 974-979
    • Cary, R.B.1    Chen, F.2    Shen, Z.3    Chen, D.J.4
  • 35
    • 0032573467 scopus 로고    scopus 로고
    • Subcellular localization and protein-protein interaction regions of Ku proteins
    • Koike, M., Miyasaka, T., Mimori, T. and Shiomi, T. (1998) Subcellular localization and protein-protein interaction regions of Ku proteins. Biochem. Biophys. Res. Commun. 252: 679-685.
    • (1998) Biochem. Biophys. Res. Commun. , vol.252 , pp. 679-685
    • Koike, M.1    Miyasaka, T.2    Mimori, T.3    Shiomi, T.4
  • 36
    • 0031974492 scopus 로고    scopus 로고
    • Identification of two domains of the p70 Ku protein mediating dimerization with p80 and DNA binding
    • Wang, J., Dong, X., Myung, K., Hendrickson, E. A. and Reeves, W. H. (1998) Identification of two domains of the p70 Ku protein mediating dimerization with p80 and DNA binding. J. Biol. Chem. 273: 842-848.
    • (1998) J. Biol. Chem. , vol.273 , pp. 842-848
    • Wang, J.1    Dong, X.2    Myung, K.3    Hendrickson, E.A.4    Reeves, W.H.5
  • 37
    • 0035815607 scopus 로고    scopus 로고
    • Dimerization and nuclear localization of ku proteins
    • Koike, M., Shiomi, T. and Koike, A. (2001) Dimerization and nuclear localization of ku proteins. J. Biol. Chem. 276: 11167-11173.
    • (2001) J. Biol. Chem. , vol.276 , pp. 11167-11173
    • Koike, M.1    Shiomi, T.2    Koike, A.3
  • 39
    • 0033490108 scopus 로고    scopus 로고
    • Differential subcellular localization of DNA-dependent protein kinase components Ku and DNA-PKcs during mitosis
    • Koike, M., Awaji, T., Kataoka, M., Tsujimoto, G., Kartasova, T., Koike, A. and Shiomi, T. (1999) Differential subcellular localization of DNA-dependent protein kinase components Ku and DNA-PKcs during mitosis. J. Cell Sci. 112: 4031-4039.
    • (1999) J. Cell Sci. , vol.112 , pp. 4031-4039
    • Koike, M.1    Awaji, T.2    Kataoka, M.3    Tsujimoto, G.4    Kartasova, T.5    Koike, A.6    Shiomi, T.7
  • 40
    • 0021063154 scopus 로고
    • X-ray-sensitive mutants of Chinese hamster ovary cell line. Isolation and cross-sensitivity to other DNA-damaging agents
    • Jeggo, P. A. and Kemp, L. M. (1983) X-ray-sensitive mutants of Chinese hamster ovary cell line. Isolation and cross-sensitivity to other DNA-damaging agents. Mutat. Res. 112: 313-327.
    • (1983) Mutat. Res. , vol.112 , pp. 313-327
    • Jeggo, P.A.1    Kemp, L.M.2
  • 41
    • 0028112944 scopus 로고
    • Restoration of X-ray resistance and V(D)J recombination in mutant cells by Ku cDNA
    • Smider, V., Rathmell, W. K., Lieber, M. R. and Chu, G. (1994) Restoration of X-ray resistance and V(D)J recombination in mutant cells by Ku cDNA. Science 266: 288-291.
    • (1994) Science , vol.266 , pp. 288-291
    • Smider, V.1    Rathmell, W.K.2    Lieber, M.R.3    Chu, G.4
  • 44
    • 0034646304 scopus 로고    scopus 로고
    • Defective embryonic neurogenesis in Ku-deficient but not DNA-dependent protein kinase catalytic subunit-deficient mice
    • Gu, Y., Sekiguchi, J., Gao, Y., Dikkes, P., Frank, K., Ferguson, D., Hasty, P., Chun, J. and Alt, F. W. (2000) Defective embryonic neurogenesis in Ku-deficient but not DNA-dependent protein kinase catalytic subunit-deficient mice. Proc. Natl. Acad. Sci. USA 97: 2668-2673.
    • (2000) Proc. Natl. Acad. Sci. USA , vol.97 , pp. 2668-2673
    • Gu, Y.1    Sekiguchi, J.2    Gao, Y.3    Dikkes, P.4    Frank, K.5    Ferguson, D.6    Hasty, P.7    Chun, J.8    Alt, F.W.9
  • 46
    • 0037108857 scopus 로고    scopus 로고
    • An antisense oligonucleotide targeted to human Ku86 messenger RNA sensitizes M059K malignant glioma cells to ionizing radiation, bleomycin, and etoposide but not DNA cross-linking agents
    • Belenkov, A. I., Paiement, J. P., Panasci, L. C., Monia, B. P. and Chow, T. Y. (2002) An antisense oligonucleotide targeted to human Ku86 messenger RNA sensitizes M059K malignant glioma cells to ionizing radiation, bleomycin, and etoposide but not DNA cross-linking agents. Cancer Res. 62: 5888-5896.
    • (2002) Cancer Res. , vol.62 , pp. 5888-5896
    • Belenkov, A.I.1    Paiement, J.P.2    Panasci, L.C.3    Monia, B.P.4    Chow, T.Y.5
  • 47
    • 0038407413 scopus 로고    scopus 로고
    • Adenovirus-mediated heat-activated antisense Ku70 expression radiosensitizes tumor cells in vitro and in vivo
    • Li, G. C., He, F., Shao, X., Urano, M., Shen, L., Kim, D., Borrelli, M., Leibel, S. A., Gutin, P. H. and Ling, C. C. (2003) Adenovirus-mediated heat-activated antisense Ku70 expression radiosensitizes tumor cells in vitro and in vivo. Cancer Res. 63: 3268-3274.
    • (2003) Cancer Res. , vol.63 , pp. 3268-3274
    • Li, G.C.1    He, F.2    Shao, X.3    Urano, M.4    Shen, L.5    Kim, D.6    Borrelli, M.7    Leibel, S.A.8    Gutin, P.H.9    Ling, C.C.10


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.