Transfer of Ku86 RNA antisense decreases the radioresistance of human fibroblasts

Cancer Gene Therapy

Volumn 7, Issue 2, 2000, Pages 339-346

  Marangoni, Elisabetta ^a   Le Romancer, Muriel ^b   Foray, Nicolas ^a   Muller, Catherine ^c   Douc Rasy, Sétha ^a   Vaganay, Sabine ^a   Abdulkarim, Bassam ^a   Barrois, Michel ^a   Calsou, Patrick ^c   Bernier, Jacques ^d   Salles, Bernard ^c   Bourhis, Jean ^a  

^a INSTITUT GUSTAVE ROUSSY   (France)

^b BICHAT HOSPITAL   (France)

^c INSTITUT DE PHARMACOLOGIE ET DE BIOLOGIE STRUCTURALE   (France)

^d Hôpital Cantonal   (Switzerland)

Author keywords

Antisense; DNA protein kinase; DNA repair; Ionizing radiation; Ku86

Indexed keywords

COMPLEMENTARY DNA; COMPLEMENTARY RNA; KU ANTIGEN; PROTEIN KINASE;

ARTICLE; CELL LEVEL; CONTROLLED STUDY; DNA STRAND BREAKAGE; ENZYME ACTIVITY; EXCISION REPAIR; FIBROBLAST; GENE TRANSFER; HUMAN; HUMAN CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN DNA BINDING; RADIATION DOSE; RADIATION RESPONSE; RADIOSENSITIVITY;

RODENTIA; SIMIAE; SIMIAN VIRUS; SIMIAN VIRUS 40;

EID: 0034127568     PISSN: 09291903     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.cgt.7700111     Document Type: Article

References (58)

1. Badie C, Iliakis G, Foray N. Induction and rejoining of DNA double-strand breaks and interphase chromosome breaks after exposure to X rays in one normal and two hypersensitive human fibroblast cell lines. Radiat Res. 1995;144:26-35.
2. Badie C, Goodhardt M, Waugh A, et al. A DNA double-strand break defective fibroblast cell line (180BR) derived from a radiosensitive patient represents a new mutant phenotype. Cancer Res. 1997;57:4600-4607.
3. Foray N, Priestley A, Alsbeih G, et al. Hypersensitivity of ataxia telangiectasia fibroblasts to ionizing radiation is associated with a repair deficiency of DNA double-strand breaks. Int J Radiat Biol. 1997;72:271-283.
4. Allalunis-Turner MJ, Zia PKY, Barron GM, Mirzayans R, Day RS III. Radiation-induced DNA damage repair in cells of a radiosensitive human malignant glioma cell line. Radiat Res. 1995;144:288-293.
5. Schwartz JL, Mustafi R, Beckett MA, Weichselbaum RR. DNA double-strand break rejoining rates, inherent radiation sensitivity, and human tumour response to radiotherapy. Br J Cancer. 1996;74:37-42.
6. Evans HH, Ricanati M, Horng MF, Jiang G, Mencl J, Olive P. DNA double-strand break rejoining deficiency in TK6 and other human B-lymphoblast cell lines. Radiat Res. 1995;134:307-315.
7. Iliakis G, Metzger L, Muschel RJ, McKenna WG. Induction and repair of DNA double strand breaks in radiation-resistant cells obtained by transformation of primary rat embryo cells with the oncogenes H-ras and V-myc. Cancer Res. 1990;50:6575-6579.
8. Debenham PG, Jones NJ, Webb MBT. Vector-mediated DNA double-strand break repair analysis in normal and radiation-sensitive Chinese hamster V9 cells. Mutat Res. 1988;203:1-9.
9. Shinohara A, Ogawa H, Matsuda Y, Ushio N, Ikeo K, Ogawa T. Cloning of human, mouse, and fission yeast recombination genes homologous to RAD51 and recA. Nat Genet. 1993;4:239-243.
10. Shen Z, Denison K, Lobb R, Gatewood JM, Chen DJ. The human and mouse homologs of the yeast RAD52 gene: cDNA cloning, sequence analysis, assignment to human chromosome 12p12.2-p13, and mRNA expression in mouse tissues. Genomics. 1995;25:199-206.
11. Petrini JHJ, Walsh ME, DiMare C, Chen XN, Korenberg JR, Weaver DT. Isolation and characterization of the human MRE11 homologue. Genomics. 1995;29:80-86.
12. Dolganov GM, Maser RS, Novikov A, et al. Human Rad50 is physically associated with human Mre11: identification of a conserved multiprotein complex implicated in recombinational DNA repair. Mol Cell Biol. 1996;16:4832-4841.
13. Essers J, Hendriks RV, Swagemakers SM, et al. Disruption of mouse RAD54 reduces ionizing radiation resistance and homologous recombination. Cell. 1997;89:195-204.
14. Masson N, Niedergang C, Schreiber V, Muller S, Menissier-de Murcia J, De Murcia G. XRCC1 is specifically associated with poly(ADP-ribose) polymerase and negatively regulates its activity following DNA damage. Mol Cell Biol. 1998;18:3563-3571.
15. Li Z, Otevrel T, Gao Y, et al. The XRCC4 gene encodes a novel protein involved in DNA double-strand break repair and V(D)J recombination. Cell. 1995;83:1079-1089.
16. Jeggo PA, Hafezparast M, Thomson M, et al. Localisation of DNA repair gene (XRCC5) involved in double-strand break rejoining to human chromosome 2. Proc Natl Acad Sci USA. 1992;89:6423-6427.
17. Jin S, Weaver DT. Double-strand break repair by Ku70 requires heterodimerization with Ku80 and DNA binding functions. EMBO J. 1997;16:6874-6885.
18. Gottlieb TM, Jackson SP. The DNA-dependent protein kinase: requirement of DNA ends and association with Ku antigen. Cell. 1993;72:131-142.
19. Lee SE, Pulaski CR, He DM, et al. Isolation of mammalian cell mutants that are X-ray sensitive, impaired in DNA double-strand break repair, and defective for V(D)J recombination. Mutat Res. 1995;336:279-291.
20. Taccioli GE, Gottlieb TM, Blunt T, et al. Ku80: product of the XRCC5 gene and its role in DNA repair and V(D)J recombination. Science. 1994;265:1442-1445.
21. Smider V, Rathmell WK, Lieber MR, Chu G. Restoration of X-ray resistance and V(D)J recombination in mutant cells by Ku cDNA. Science. 1994;266:288-291.
22. Mimori T, Akizuki M, Yamagata H, Inada S, Yoshida S, Homma M. Characterization of a high molecular weight acidic nuclear protein recognized by autoantibodies in sera from patients with polymyositis-scleroderma overlap. J Clin Invest. 1981;68:611-620.
23. Mimori T, Hardin JA. Mechanism of interaction between Ku protein and DNA. J Biol Chem. 1986;261:10375-10379.
24. Falzon M, Kuff EL. Binding of the transcription factor EBP-80 mediates the methylation response of an intracisternal A-particle long terminal repeat promoter. Mol Cell Biol. 1991;11:117-125.
25. Blier PR, Griffith AJ, Craft J, Hardin JA. Binding of Ku protein to DNA: measurement of affinity for ends and demonstration of bindings to nicks. J Biol Chem. 1993;268: 7594-7601.
26. Cao QP, Pitt S, Leszyk J, Baril EF. DNA-dependent ATPase from HeLa cells is related to human Ku autoantigen. Biochemistry. 1994;33:8548-8557.
27. Tuteja N, Tuteja R, Ochem A, et al. Human DNA helicase II: a novel DNA unwinding enzyme identified as the Ku autoantigen. EMBO J. 1994;13:4991-5001.
28. Wu XT, Lieber M. Protein-protein and protein DNA interaction regions within the DNA end-binding protein Ku70-Ku86. Mol Cell Biol. 1996;16:5186-5193.
29. Jeggo PA. DNA-PK: at the cross-roads of biochemistry and genetics. Mutat Res. 1997;384:1-14.
30. Hartley KO, Gell D, Smith GC, et al. DNA-dependent protein kinase catalytic subunit: a relative of phosphatidylinositol 3-kinase and the ataxia telangiectasia gene product. Cell. 1995;82:849-856.
31. Anderson CW, Carter TH. The DNA-activated protein kinase, DNA-PK. In: Jessberger R, Lieber MR, eds. Current Topics in Microbiology and Immunology, Lymphocyte Neoplasia, and DNA Rearrangements in the Immune System. Berlin: Springler-Verlag; 1996:91-111.
32. Shieh SY, Ikeda M, Taya Y, Prives C. DNA damage-induced phosphorylation of p53 alleviates inhibition by MDM2. Cell. 1998;91:325-334.
33. Finnie NJ, Gottlieb TM, Blunt T, Jeggo PA, Jackson SP. DNA-dependent protein kinase activity is absent in xrs-6 cells: implications for site-specific recombination and DNA double-strand break repair. Proc Natl Acad Sci USA. 1995;92:320-324.
34. Jackson SP, Jeggo PA. DNA double-strand break repair, V(D)J recombination, and the involvement of DNA-PK. Trends Biochem Sci. 1995;20:412-415.
35. Errami A, Smider V, Rathmell WK, et al. Ku86 defines the genetic defect and restores X-ray resistance and V(D)J recombination to complementation group 5 hamster cell mutants. Mol Cell Biol. 1996;16:1519-1526.
36. Ross GM, Eady JJ, Mithal NP, et al. DNA strand break rejoining defect in xrs6 is complemented by transfection with the human Ku80 gene. Cancer Res. 1995;55:1235-1238.
37. Komatsu K, Kubota N, Gallo M, Okumura Y, Lieber MR. The scid factor on human chromosome 8 restores V(D)J recombination in addition to double-strand break repair. Cancer Res. 1995;55:1774-1779.
38. Lees-Miller SP, Godbout R, Chan DW, et al. Absence of p350 subunit of DNA-activated protein kinase from a radiosensitive human cell line. Science. 1995;267:1183-1185.
39. Chan DW, Gately DP, Urban S, et al. Lack of correlation between ATM protein expression and tumour cell radiosensitivity. Int J Radiat Biol. 1998;74:217-224.
40. Myung K, He DM, Lee SE, Hendrickson A. KARP-1: a novel leucine zipper protein expressed from the Ku86 autoantigen locus is implicated in the control of DNA-dependent protein kinase activity. EMBO J. 1997;16:3172-3184.
41. Le Romancer M, Reyl-Desmars F, Cherifi Y, et al. The 86-kDa subunit of autoantigen Ku is a somatostatin receptor regulating protein phosphatase-2A activity. J Biol Chem. 1994;269:17464-17468.
42. Hutschka LI, Holliday R. Limited and unlimited growth of SV40-transformed cells from human diploid MRC5 fibroblast. J Cell Sci. 1983;63:77-99.
43. Arlett CF, Green MHL, Priestley A, Harcourt SA, Mayn LV. Comparative human cellular radiosensitivity: the effect of the SV40 transformation and immortalisation on the γ-irradiation survival of skin-derived fibroblasts from normal individuals and from ataxia-telangiectasia patients and heterozygotes. Int J Radiat Biol. 1988;54:911-928.
44. Muller C, Dusseau C, Calsou P, Salles B. Human normal peripheral blood B-lymphocytes are deficient in DNA-dependent protein kinase activity due to the expression of a variant form of the Ku86 protein. Oncogene. 1998;16: 1553-1560.
45. Han Z, Johnston C, Reeves WH, Carter T, Wyche JH, Hendrickson EA. Characterization of a Ku86 variant protein that results in altered DNA binding and diminished DNA-dependent protein kinase activity. J Biol Chem. 1996;271:14098-14104.
46. Fertil B, Dertinger H, Courdi A, Malaise EP. Mean inactivation dose: a useful concept for intercomparison of human cell survival curves. Radiat Res. 1984;99:73-84.
47. Foray N, Badie C, Alsbeih G, Fertil B, Malaise EP. A new model describing the curves for repair of both DNA double-strand breaks and chromosome damage. Radiat Res. 1996;146:53-60.
48. Liu N, Bryant PE. Response of ataxia telangiectasia cells to restriction endonuclease-induced DNA double-strand breaks: cytogenetic characterization. Mutagenesis. 1993;8: 503-510.
49. Nussenzweig A, Sokol K, Burgman P, Li L, Li G. Hypersensitivity of Ku80-deficient cell lines and mice to DNA damage: the effects of ionizing radiation on growth, survival, and development. Proc Natl Acad Sci USA. 1997;94: 13588-13593.
50. Singleton BK, Priestley A, Steingrimsdottir H, et al. Molecular and biochemical characterization of xrs mutants defective in Ku80. Mol Cell Biol. 1997;17:1264-1273.
51. Ramsden DA, Gellert M. Ku protein stimulates DNA end joining by mammalian DNA ligase: a direct role for Ku in repair of DNA double-strand breaks. EMBO J. 1998;17: 609-614.
52. Dynan WS, Yoo S. Interaction of Ku protein and DNA-dependent protein kinase catalytic subunit with nucleic acids. Nucleic Acids Res. 1998;26:1551-1559.
53. Yaneva M, Kowalewski T, Lieber MR. Interaction of DNA-dependent protein kinase with DNA and with Ku: biochemical and atomic-force microscopy studies. EMBO J. 1997;16:5098-5112.
54. Hammersten O, Chu G. DNA-dependent protein kinase: DNA binding and activation in the absence of Ku. Proc Natl Acad Sci USA. 1998;95:525-530.
55. Urhammer N, Fritz E, Boyden L, Meyn MS. An ATM antisense vector confers ataxia-telangiectasia phenotype on normal cell. Am J Hum Genet. 1996;59S:A4. Abstract 10.
56. Grote SJ, Joshi GP, Revell SH, Shaw CA. Observations of radiation-induced chromosome fragment loss in live mammalian cells in culture, and its effect in colony-forming ability. Int J Radiat Biol. 1981;39:395-408.
57. 1-phase Chinese hamster ovary cells exposed to charged-particle beams. Radiat Res. 1994; 138:343-351.
58. Johnston PJ, Stoppard E, Bryant PE. Induction and distribution of damage in CHO-K1 and the X-ray-sensitive hamster cell line xrs5, measured by the cytochalasin-B-cytokinesis block micronucleus assay. Mutat Res. 1997;385: 1-12.