Differential subcellular localization of DNA-dependent protein kinase components Ku and DNA-PKcs during mitosis

Journal of Cell Science

Volumn 112, Issue 22, 1999, Pages 4031-4039

  Koike, Manabu ^a   Awaji, Takeo ^b   Kataoka, Masakazu ^b   Tsujimoto, Gozoh ^b   Kartasova, Tonja ^c   Koike, Aki ^a   Shiomi, Tadahiro ^a  

^a NATIONAL INSTITUTE OF RADIOLOGICAL SCIENCES   (Japan)

^b NATIONAL RESEARCH INSTITUTE FOR CHILD HEALTH AND DEVELOPMENT   (Japan)

^c NATIONAL CANCER INSTITUTE   (United States)

Author keywords

Cell cycle; Chromosome periphery; DNA PK; Ku70; Ku80; Localization

Indexed keywords

DIMER; DNA DEPENDENT PROTEIN KINASE; DOUBLE STRANDED DNA; KU ANTIGEN; PROTEIN SUBUNIT;

ARTICLE; CELL CYCLE; CELL CYCLE G2 PHASE; CELL LINE; CELL NUCLEUS; CELLULAR DISTRIBUTION; CHROMOSOME CONDENSATION; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; CYTOPLASM; DNA REPAIR; GROWTH REGULATION; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; IMMUNOPRECIPITATION; INTERPHASE; METAPHASE CHROMOSOME; MITOSIS; PRIORITY JOURNAL;

ANTIGENS, NUCLEAR; CALCIUM-BINDING PROTEINS; CELL CYCLE; CELL CYCLE PROTEINS; CELL LINE; CELL NUCLEUS; CHROMOSOMES, HUMAN; DNA HELICASES; DNA-ACTIVATED PROTEIN KINASE; DNA-BINDING PROTEINS; FLUORESCENT ANTIBODY TECHNIQUE; HELA CELLS; HUMANS; K562 CELLS; METAPHASE; MICROSCOPY, CONFOCAL; MITOSIS; NUCLEAR PROTEINS; PROTEIN-SERINE-THREONINE KINASES; TUMOR CELLS, CULTURED;

EID: 0033490108     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (49)

1. Bakalkin, G., Yakovleva, T., Hurd, Y. L., Nussenzweig, A., Li, G. C. and Terenius, L. (1998). Autoantigen Ku in the brain developmentally regulated expression and subcellular localization. Neuroreport 9, 2147-2151.
2. Barnes, G. and Rio, D. (1997). DNA double-strand-break sensitivity, DNA replication, and cell cycle arrest phenotypes of Ku-deficient Saccharomyces cerevisiae. Proc. Nat. Acad. Sci. USA 94, 867-872.
3. Boulton, S. J. and Jackson, S. P. (1996). Identification of a Saccharomyces cerevisiae Ku80 homologue: roles in DNA double strand break rejoining and in telomeric maintenance. Nucl. Acids Res. 24, 4639-4648.
4. Cai, Q.-Q., Plet A., Imbert, J., Lafage-Pochitaloff, M., Cerdan, C. and Blanchard, J.-M. (1994). Chromosomal legation and expression of the genes coding for Ku p70 and p80 in human cell lines and normal tissues. Cytogenet. Cell Genet. 65, 221-227.
5. Carter, T., Vancurova, I., Sun, I., Lou, W. and DeLeon, S. (1990). A DNA-activated protein kinase from HeLa cell nuclei. Mol. Cell Biol. 10, 6460-6471.
6. Dalziel, R. G., Mendelson, S. C. and Quinn, J. P. (1992). The nuclear autoimmune antigen Ku is also present on the cell surface. Autoimmunity 13, 265-267.
7. Dynan, W. S. and Yoo, S. (1998). Interaction of Ku protein and DNA-dependent protein kinase catalytic subunit with nucleic acids. Nucl. Acids Rev. 26, 1551-1559.
8. Errami A, Smider, V., Rathmell, W. K., He, D. M., Hendrickson, E. M., Zdzienicka, M. Z. and Chu, G. (1996). Ku86 defines the genetic defect and restores X-ray resistance and V(D)J recombination to complementation group 5 hamster cell mutants. Mol. Cell. Biol. 16, 1519-1526.
9. Fewell, J. W. and Kuff, E. L. (1996). Intracellular redistribution of Ku immunoreactivity in response to cell-cell contact and growth modulating components in the medium. J. Cell Sci. 109, 1937-1946.
10. Finnie, N. J., Gottlieb, T. M., Blunt, T., Jeggo, P. A., Jackson, S. P. (1995). DNA-dependent protein kinase activity is absent in xrs-6 cells: implications for site-specific recombination and DNA double-strand break repair. Proc. Nat. Acad. Sci. USA 92, 320-324.
11. Gao, Y., Chaudhuri, J., Zhu, C., Davidson, L., Weaver, D. T., Alt, F. W. (1998). A targeted DNA-PKcs-null mutation reveals DNA-PK-independent functions for KU in V(D)J recombination. Immunity 9, 367-376.
12. Gottlieb, T. M. and Jackson, S. P. (1993). The DNA-dependent protein kinase: requirement for DNA ends and association with Ku antigen. Cell 72, 131-142.
13. Grawunder, U., Finnie, N., Jackson, S. P., Riwar, B., Jessberger, R., Reeves, W. H., Satoh, M., Wang, J., Chou, C. H. and Ajmani, A. K. (1996) Expression of DNA-dependent protein kinase holoenzyme upon induction of lymphocyte differentiation and V(D)J recombination. Eur. J. Biochem. 241, 931-940.
14. Gu, Y., Seidl, K. J., Rathbun, G. A., Zhu, C., Manis, J. P., van der Stoep, N., Davidson, L., Cheng, H. L., Sekiguchi, J. M., Frank, K., Stanhope-Baker, P., Schlissel, M. S., Roth, D. B. and Alt, F. W. (1997a). Growth retardation and leaky SCID phenotype of Ku70-deficient mice. Immunity 7, 653-665.
15. Gu, Y., Jin, S., Gao, Y., Weaver, D. T. and Alt, F. W. (1997b). Ku70-deficient embryonic stem cells have increased ionizing radiosensitivity, defective DNA end-binding activity and inability to support V(D)J recombination. (1997b). Proc. Nat. Acad. Sci. USA 94, 8076-8081.
16. Han, Z., Johnston, C., Reeves, W. H., Carter, T., Wyche, J. H. and Hendrickson, E. A. Characterization of a Ku86 variant protein that results in altered DNA binding and diminished DNA-dependent protein kinase activity. (1996). J. Biol. Chem. 271, 14098-14104.
17. Higashiura, M., Shimizu, Y., Tanimoto, M., Morita, T., and Yagura, T. (1992). Immunolocalization of Ku-proteins (p80/p70): localization of p70 to nucleoli and periphery of both interphase nuclei and metaphase chromosomes. Exp. Cell. Res. 201, 444-451.
18. Keegan, K. S., Holtzman, D. A., Plug, A. W., Christenson, E. R., Brainerd, E. E., Flaggs, G., Bentley, N. J., Taylor, E. M., Meyn, M. S., Moss, S. B., Carr, A. M., Ashley, T. and Hoekstra, M. F. (1996). The Atr and Atm protein kinases associate with different sites along meiotically pairing chromosomes. Genes Dev. 10, 2423-2437.
19. Kleinschmidt, J. A. and Steinbeisser, H. (1991). DNA-dependent phosphorylation of histone H2A. X during nucleosome assembly in Xenopus laevis oocytes: involvement of protein phosphorylation in nucleosome spacing. EMBO J. 10, 3043-3050.
20. Koike, M., Kuroki, T. and Nose, K. (1993). Common target for 12-O-tetradecanoyl-phorbol-13-acetate and ras in the transcriptional enhancer of the growth factor-inducible JE gene. Mol. Carcinog. 8, 105-111.
21. Koike, M., Ishino, K., Huh, N. and Kuroki, T. (1994). Growth inhibition of SV40-transformed human keratinocytes by TGF-beta s is not linked to dephosphorylation of the Rb gene product. Biochem. Biophys. Res. Commun. 201, 673-681.
22. Koike, M., Ishino, K., Ikuta, T., Huh, N. and Kuroki, T. (1995). Growth enhancement of normal human keratinocytes by the antisense oligonucleotide of retinoblastoma susceptibility gene. Oncogene 10, 117-122.
23. Koike, M., Matsuda, Y., Mimori, T., Harada, Y-N., Shiomi, N. and Shiomi, T. (1996a). Chromosomal localization of the mouse and rat DNA double-strand break repair genes Ku p70 and Ku p80/XRCC5 and their mRNA expression in various mouse tissues. Genomics 38, 38-44.
24. Koike, M., Tshino, K., Kohno, Y., Tachikawa, T., Kartasova, T., Kuroki, T. and Huh, N. (1996b). DMSO induces apoptosis in SV40-transformed human keratinocytes, but not in normal keratinocytes. Cancer Lett. 108, 185-193
25. Koike, M., Miyasaka, T., Mimori, T. and Shiomi, T. (1998). Subcellular localization and protein-protein interaction regions of Ku proteins. Biochem. Biophys. Res. Commun. 252, 679-685.
26. Lee, S. E., Mitchell, R. A., Cheng, A. and Hendrickson, E. A. (1997). Evidence for DNA-PK-dependent and-independent DNA double-strand break repair pathways in mammalian cells as a function of the cell cycle. Mol. Cell. Biol. 17, 1425-1433.
27. Lees-Miller, S. P., Chen, Y. R. and Anderson, C. W. (1990). Human cells contain a DNA-activated protein kinase that phosphorylates simian virus 40 T antigen, mouse p53 and the human Ku autoantigen. Mol. Cell. Biol. 10, 6472-6481.
28. Li, L. L. and Yen, N. H. (1992). Cell cycle-dependent migration of the DNA-binding protein Ku80 into nucleoli. Exp. Cell. Res. 199, 262-268.
29. Liang, F. and Jasin, M. (1996). Ku80-deficient cells exhibit excess degradation of extrachromosomal DNA. J. Biol. Chem. 271, 14405-14411.
30. Mimori, T., Hardin, J. A. and Steitz, J. A. (1986). Characterization of the DNA-binding protein antigen Ku recognized by autoantibodies from patients with rheumatic disorders. J. Biol. Chem. 261, 2274-2278.
31. 2 and M phases of the cell cycle. Mol. Cell. Biol. 18, 5797-5808.
32. Nussenzweig, A., Chen, C., da Costa Soares, V., Sanchez, M., Sokol, K., Nussenzweig, M. C. and Li, G. C. (1996). Requirement for Ku80 in growth and immunoglobulin V(D)J recombination. Nature 382, 551-555.
33. Ouyang, H., Nussenzweig, A., Kurimasa, A., Soares, V. C., Li, X., Cordon-Cardo, C., Li, W. H., Cheong, N., Nussenzweig, M., Iliakis, G., Chen, D. J. and Li, G. C. (1997). Ku70 is required for DNA repair but not for T cell antigen receptor gene recombination In vivo. J. Exp. Med. 186, 921-929.
34. Pines, J. and Hunier, T. (1989). Isolation of a human cyclin cDNA: evidence for cyclin mRNA and protein regulation in the cell cycle and for interaction with p34cdc2. Cell 58, 833-846.
35. Porter, S. E., Greenwell, P. W., Ritchie, K. B. and Petes, T. D. (1996). The DNA-binding prolein Hdf1p (a putative Ku homologue) is required for maintaining normal telomere length in Saccharomyces cerevisiae. Nucl. Acids Res. 24, 582-585.
36. Prabhakar, B. S., Allaway, G. P., Srinivasappa, J. and Notkins, A. L. (1990). Cell surface expression of the 70-kD component of Ku, a DNA-binding nuclear autoantigen. J. Clin. Invest 86, 1301-1305.
37. Reeves, W. H. (1987). Antinuclear antibodies as probes to explore the structural organization of the genome. J. Rheumatol. 14 (suppl 13), 97-105.
38. Reeves, W. H. (1992). Antibodies to the p70/p80 (Ku) antigens in systemic lupus erythematosus. Rheum. Dis. Clin. North. Am. 18, 391-414.
39. Reeves, W. H., Satoh, M., Wang, J., Chou, C. H. and Ajmani, A. K. (1994). Systemic lupus erythemalosus. Antibodies to DNA, DNA-binding proteins and histones. Rheum. Dis. Clin. North. Am. 20, 1-28.
40. Reeves, W. H., Wang, J., Ajimani, A. K., Stojanov, L. and Satoh, M. (1997). The Ku autoantigen. In The Antibodies (ed. M. Zanetti and J. Capra), pp. 33-84. Amsterdam: Harwood Academic Publishers.
41. Satoh, M., Wang. J. and Reeves, W. H. (1995). Role of free p70 (Ku) subunit in posttranslational stabilization of newly synthesized p80 during DNA-dependent protein kinase assembly. Eur. J. Cell. Biol. 66, 127-135.
42. Singleton, B. K., Priestley, A., Steingrimsdottir, H., Gell, D., Blunt, T., Jackson, S. P., Lehmann, A. R. and Jeggo, P. A. (1997). Molecular and biochemical characterization of xrs mutants defective in Ku80. Mol. Cell Biol. 17, 1264-1273.
43. Todorov, I. T., Attaran, A. and Kearsey, S. E. (1995). BM28, a human member of the MCM2-3-5 family, is displaced from chromatin during DNA replication. J. Cell Biol. 129, 1433-1445.
44. Tsukamoto, Y., Kato, J. and Ikeda, H. (1997). Silencing factors participate in DNA repair and recombination in Saccharomyces cerevisiae. Nature 388, 900-903.
45. Wang, J., Chou, C. H., Blankson, J., Satoh, M., Knuth, M. W., Eisenberg, R. A., Pisetsky, D. S. and Reeves, W. H. (1993). Murine monoclonal antibodies specific for conserved and non-conserved antigenic determinants of the human and murine Ku autoantigens. Mol. Biol. Rep. 18, 15-28.
46. Watanabe, F., Shirakawa, H., Yoshida, M., Tsukada, K. and Teraoka, H. (1994). Stimulation of DNA-dependent protein kinase activity by high mobility group proteins 1 and 2. Biochem. Biophys. Res. Commun. 202, 736-742.
47. Yaneva, M., Ochs, R., McRorie, D. K., Zweig, S. and Busch, H. (1985). Purification of an 86-70 kDa nuclear DNA-associated protein complex. Biochim. Biophys. Acta. 841, 22-29.
48. Yaneva, M. and Busch, H. (1986). A 10S particle released from deoxyribonuclease-sensitive regions of HeLa cell nuclei contains the 86-kilodalton-70-kilodalton protein complex. Biochemistry 25, 5057-5063.
49. Yaneva, M. and Jiang, S. (1991). Expression of the Ku protein during cell proliferation. Biochim. Biophys. Acta. 1090, 181-187.