Ku70 can translocate to the nucleus independent of Ku80 translocation and DNA-PK autophosphorylation

Biochemical and Biophysical Research Communications

Volumn 276, Issue 3, 2000, Pages 1105-1111

  Koike, Manabu ^a   Shiomi, Tadahiro ^a   Koike, Aki ^a  

^a NATIONAL INSTITUTE OF RADIOLOGICAL SCIENCES   (Japan)

Author keywords

DNA PK; GFP; Heterodimer; Ku; NLS

Indexed keywords

DNA DEPENDENT PROTEIN KINASE; KU ANTIGEN;

ANIMAL CELL; ARTICLE; AUTOPHOSPHORYLATION; BINDING AFFINITY; CELL NUCLEUS; DIMERIZATION; ENZYME ACTIVITY; HAMSTER; HUMAN; HUMAN CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; PROTEIN STRUCTURE; PROTEIN TRANSPORT; REGULATORY MECHANISM; SITE DIRECTED MUTAGENESIS;

EID: 0034609947     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1006/bbrc.2000.3567     Document Type: Article

References (33)

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2. Human cells contain a DNA-activated protein kinase that phosphorylates simian virus 40 T antigen, mouse p53, and the human Ku autoantigen
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4. DNA-dependent protein kinase phosphorylation sites in Ku 70/80 heterodimer
5. Requirement for Ku80 in growth and immunoglobulin V(D)J recombination
6. Growth retardation and leaky SCID phenotype of Ku70-deficient mice
7. Ku70 is required for DNA repair but not for T cell antigen receptor gene recombination in vivo
8. A targeted DNA-PKcs-null mutation reveals DNA-PK-independent functions for Ku in V(D)J recombination
9. Ku86 defines the genetic defect and restores X-ray resistance and V(D)J recombination to complementation group 5 hamster cell mutants
10. Molecular and biochemical characterization of xrs mutants defective in Ku80
11. Protein-protein and protein-DNA interaction regions within the DNA end-binding protein Ku70-Ku86
12. Defining the minimal domain of Ku80 for interaction with Ku70
13. A central region of Ku80 mediates interaction with Ku70 in vivo
14. Subcellular localization and protein-protein interaction regions of Ku proteins
15. Identification of two domains of the p70 Ku protein mediating dimerization with p80 and DNA binding
16. Ku70: A candidate tumor suppressor gene for murine T cell lymphoma
17. Cell surface expression of the 70-kD component of Ku, a DNA-binding nuclear autoantigen
18. The nuclear autoimmune antigen Ku is also present on the cell surface
19. Antibodies to the p70/p80 (Ku) antigens in systemic lupus erythematosus
20. Expression of DNA-dependent protein kinase holoenzyme upon induction of lymphocyte differentiation and V(D)J recombination
21. The 86-kDa subunit of autoantigen Ku is a somatostatin receptor regulating protein phosphatase-2A activity
22. Intracellular redistribution of Ku immunoreactivity in response to cell-cell contact and growth modulating components in the medium
23. Differential subcellular localization of DNA-dependent protein kinase components Ku and DNA-PKcs during mitosis
24. The nuclear localization signal of the human Ku70 is a variant bipartite type recognized by the two components of nuclear pore-targeting complex
25. Ku80 can translocate to the nucleus independent of the translocation of Ku70 using its own nuclear localization signal
26. The C terminus of Ku80 activates the DNA-dependent protein kinase catalytic subunit
27. Growth enhancement of normal human keratinocytes by the antisense oligonucleotide of retinoblastoma susceptibility gene
28. Double-strand break repair by Ku70 requires heterodimerization with Ku80 and DNA binding functions
29. The regulation of protein transport to the nucleus by phosphorylation
30. SV40 large tumor antigen nuclear import is regulated by the double-stranded DNA-dependent protein kinase site (serine 120) flanking the nuclear localization sequence
31. Phosphorylation on protein kinase C sites inhibits nuclear import of lamin B2
32. Scid cells are deficient in Ku and replication protein A phosphorylation by the DNA-dependent protein kinase
33. The DNA-dependent protein kinase is inactivated by autophosphorylation of the catalytic subunit