A simple and rapid method to measure cholesterol binding to P450s and other proteins

Journal of Lipid Research

Volumn 46, Issue 7, 2005, Pages 1561-1568

  Mast, Natalia ^a   Pikuleva, Irina A ^a  

^a University of Texas Medical Branch School of Medicine   (United States)

Author keywords

Alternative assay; Cytochrome P450 11A1; Cytochrome P450 46A1; Cytochrome P450 7A1; Spectral binding

Indexed keywords

CHOLESTEROL; CHOLESTEROL 7ALPHA MONOOXYGENASE; CYTOCHROME P450; CYTOCHROME P450 11A1; CYTOCHROME P450 46A1; PROTEIN; UNCLASSIFIED DRUG; 2 HYDROXYPROPYL BETA CYCLODEXTRIN; 2-HYDROXYPROPYL-BETA-CYCLODEXTRIN; BETA CYCLODEXTRIN DERIVATIVE; CHOLESTEROL 24 HYDROXYLASE; CHOLESTEROL 24-HYDROXYLASE; CHOLESTEROL MONOOXYGENASE (SIDE CHAIN CLEAVING); STEROID MONOOXYGENASE;

ARTICLE; BINDING AFFINITY; BINDING ASSAY; ENZYME SUBSTRATE COMPLEX; HYDROPHOBICITY; ISOTOPE LABELING; LIPID ANALYSIS; MATERIAL STATE; MEMBRANE BINDING; MEMBRANE FILTER; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN LIPID INTERACTION; RADIOACTIVITY; VALIDITY; CHEMISTRY; FILTRATION; METABOLISM; METHODOLOGY;

BETA-CYCLODEXTRINS; CHOLESTEROL; CHOLESTEROL 7-ALPHA-HYDROXYLASE; CHOLESTEROL SIDE-CHAIN CLEAVAGE ENZYME; CYTOCHROME P-450 ENZYME SYSTEM; FILTRATION; MICROCHEMISTRY; PROTEIN BINDING; STEROID HYDROXYLASES;

EID: 24744453987     PISSN: 00222275     EISSN: 15397262     Source Type: Journal    
DOI: 10.1194/jlr.D500008-JLR200     Document Type: Article

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