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Volumn 274, Issue 5285, 1996, Pages 255-259

Cholesterol modification of hedgehog signaling proteins in animal development

Author keywords

[No Author keywords available]

Indexed keywords

CHOLESTEROL; PROTEIN; SIGNAL PEPTIDE;

EID: 0029844192     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.274.5285.255     Document Type: Article
Times cited : (1149)

References (68)
  • 2
    • 0024254534 scopus 로고    scopus 로고
    • J. Mohler, Genetics 120, 1061 (1988); J. J. Lee, D. P. von Kessler, S. Parks, P. A. Beachy, Cell 71, 33 (1992); T. Tabata, S. Eaton, T. B. Kornberg, Genes Dev. 6, 2635 (1992); S. Tashiro et al., Gene 124, 183 (1993); P. W. Ingham, Nature 366, 560 (1993); J. Mohler and K. Vani, Development 115, 957 (1992); C. Ma, Y. Zhou, P. A. Beachy, K. Moses, Cell 75, 927 (1993); U. Heberlein, T. Wolff, G. Rubin, ibid., p. 913; Y. Echelard et al., ibid., p. 1417; R. D. Riddle, R. L. Johnson, E. Laufer, C. Tabin, ibid., p. 1401; S. Krauss, J.-P. Concordet, P. W. Ingham, ibid., p. 1431; H. Roelink et al., ibid. 76, 761 (1994); D. T. Chang et al., Development 120, 3339 (1994); K. Basler and G. Struhl, Nature 368, 208 (1994); T. Tabata and T. B. Komberg, Cell 76, 89 (1994); J. Heemskerkand S. DiNardo, ibid., p. 449; C.-M. Fan and M. Tessier-Lavigne, ibid. 79, 1175 (1994); R. L. Johnson, E. Laufer, R. D. Riddle, C. Tabin, ibid., p. 1165; M. Hynes et al., Neuron 15, 35 (1995); S. C. Ekker et al., Development 121, 2337 (1995); R. Macdonald et al., ibid., p. 3267; S. C. Ekker et al., Curr. Biol. 5, 944 (1995); C.-J. Lai, S. C. Ekker, P. A. Beachy, R. T. Moon, Development 121, 2349 (1995); J. Ericson et al., Cell 81, 747 (1995).
    • (1988) Genetics , vol.120 , pp. 1061
    • Mohler, J.1
  • 3
    • 0026783674 scopus 로고
    • J. Mohler, Genetics 120, 1061 (1988); J. J. Lee, D. P. von Kessler, S. Parks, P. A. Beachy, Cell 71, 33 (1992); T. Tabata, S. Eaton, T. B. Kornberg, Genes Dev. 6, 2635 (1992); S. Tashiro et al., Gene 124, 183 (1993); P. W. Ingham, Nature 366, 560 (1993); J. Mohler and K. Vani, Development 115, 957 (1992); C. Ma, Y. Zhou, P. A. Beachy, K. Moses, Cell 75, 927 (1993); U. Heberlein, T. Wolff, G. Rubin, ibid., p. 913; Y. Echelard et al., ibid., p. 1417; R. D. Riddle, R. L. Johnson, E. Laufer, C. Tabin, ibid., p. 1401; S. Krauss, J.-P. Concordet, P. W. Ingham, ibid., p. 1431; H. Roelink et al., ibid. 76, 761 (1994); D. T. Chang et al., Development 120, 3339 (1994); K. Basler and G. Struhl, Nature 368, 208 (1994); T. Tabata and T. B. Komberg, Cell 76, 89 (1994); J. Heemskerkand S. DiNardo, ibid., p. 449; C.-M. Fan and M. Tessier-Lavigne, ibid. 79, 1175 (1994); R. L. Johnson, E. Laufer, R. D. Riddle, C. Tabin, ibid., p. 1165; M. Hynes et al., Neuron 15, 35 (1995); S. C. Ekker et al., Development 121, 2337 (1995); R. Macdonald et al., ibid., p. 3267; S. C. Ekker et al., Curr. Biol. 5, 944 (1995); C.-J. Lai, S. C. Ekker, P. A. Beachy, R. T. Moon, Development 121, 2349 (1995); J. Ericson et al., Cell 81, 747 (1995).
    • (1992) Cell , vol.71 , pp. 33
    • Lee, J.J.1    Von Kessler, D.P.2    Parks, S.3    Beachy, P.A.4
  • 4
    • 0027096741 scopus 로고
    • J. Mohler, Genetics 120, 1061 (1988); J. J. Lee, D. P. von Kessler, S. Parks, P. A. Beachy, Cell 71, 33 (1992); T. Tabata, S. Eaton, T. B. Kornberg, Genes Dev. 6, 2635 (1992); S. Tashiro et al., Gene 124, 183 (1993); P. W. Ingham, Nature 366, 560 (1993); J. Mohler and K. Vani, Development 115, 957 (1992); C. Ma, Y. Zhou, P. A. Beachy, K. Moses, Cell 75, 927 (1993); U. Heberlein, T. Wolff, G. Rubin, ibid., p. 913; Y. Echelard et al., ibid., p. 1417; R. D. Riddle, R. L. Johnson, E. Laufer, C. Tabin, ibid., p. 1401; S. Krauss, J.-P. Concordet, P. W. Ingham, ibid., p. 1431; H. Roelink et al., ibid. 76, 761 (1994); D. T. Chang et al., Development 120, 3339 (1994); K. Basler and G. Struhl, Nature 368, 208 (1994); T. Tabata and T. B. Komberg, Cell 76, 89 (1994); J. Heemskerkand S. DiNardo, ibid., p. 449; C.-M. Fan and M. Tessier-Lavigne, ibid. 79, 1175 (1994); R. L. Johnson, E. Laufer, R. D. Riddle, C. Tabin, ibid., p. 1165; M. Hynes et al., Neuron 15, 35 (1995); S. C. Ekker et al., Development 121, 2337 (1995); R. Macdonald et al., ibid., p. 3267; S. C. Ekker et al., Curr. Biol. 5, 944 (1995); C.-J. Lai, S. C. Ekker, P. A. Beachy, R. T. Moon, Development 121, 2349 (1995); J. Ericson et al., Cell 81, 747 (1995).
    • (1992) Genes Dev. , vol.6 , pp. 2635
    • Tabata, T.1    Eaton, S.2    Kornberg, T.B.3
  • 5
    • 0027474898 scopus 로고
    • J. Mohler, Genetics 120, 1061 (1988); J. J. Lee, D. P. von Kessler, S. Parks, P. A. Beachy, Cell 71, 33 (1992); T. Tabata, S. Eaton, T. B. Kornberg, Genes Dev. 6, 2635 (1992); S. Tashiro et al., Gene 124, 183 (1993); P. W. Ingham, Nature 366, 560 (1993); J. Mohler and K. Vani, Development 115, 957 (1992); C. Ma, Y. Zhou, P. A. Beachy, K. Moses, Cell 75, 927 (1993); U. Heberlein, T. Wolff, G. Rubin, ibid., p. 913; Y. Echelard et al., ibid., p. 1417; R. D. Riddle, R. L. Johnson, E. Laufer, C. Tabin, ibid., p. 1401; S. Krauss, J.-P. Concordet, P. W. Ingham, ibid., p. 1431; H. Roelink et al., ibid. 76, 761 (1994); D. T. Chang et al., Development 120, 3339 (1994); K. Basler and G. Struhl, Nature 368, 208 (1994); T. Tabata and T. B. Komberg, Cell 76, 89 (1994); J. Heemskerkand S. DiNardo, ibid., p. 449; C.-M. Fan and M. Tessier-Lavigne, ibid. 79, 1175 (1994); R. L. Johnson, E. Laufer, R. D. Riddle, C. Tabin, ibid., p. 1165; M. Hynes et al., Neuron 15, 35 (1995); S. C. Ekker et al., Development 121, 2337 (1995); R. Macdonald et al., ibid., p. 3267; S. C. Ekker et al., Curr. Biol. 5, 944 (1995); C.-J. Lai, S. C. Ekker, P. A. Beachy, R. T. Moon, Development 121, 2349 (1995); J. Ericson et al., Cell 81, 747 (1995).
    • (1993) Gene , vol.124 , pp. 183
    • Tashiro, S.1
  • 6
    • 0027761917 scopus 로고
    • J. Mohler, Genetics 120, 1061 (1988); J. J. Lee, D. P. von Kessler, S. Parks, P. A. Beachy, Cell 71, 33 (1992); T. Tabata, S. Eaton, T. B. Kornberg, Genes Dev. 6, 2635 (1992); S. Tashiro et al., Gene 124, 183 (1993); P. W. Ingham, Nature 366, 560 (1993); J. Mohler and K. Vani, Development 115, 957 (1992); C. Ma, Y. Zhou, P. A. Beachy, K. Moses, Cell 75, 927 (1993); U. Heberlein, T. Wolff, G. Rubin, ibid., p. 913; Y. Echelard et al., ibid., p. 1417; R. D. Riddle, R. L. Johnson, E. Laufer, C. Tabin, ibid., p. 1401; S. Krauss, J.-P. Concordet, P. W. Ingham, ibid., p. 1431; H. Roelink et al., ibid. 76, 761 (1994); D. T. Chang et al., Development 120, 3339 (1994); K. Basler and G. Struhl, Nature 368, 208 (1994); T. Tabata and T. B. Komberg, Cell 76, 89 (1994); J. Heemskerkand S. DiNardo, ibid., p. 449; C.-M. Fan and M. Tessier-Lavigne, ibid. 79, 1175 (1994); R. L. Johnson, E. Laufer, R. D. Riddle, C. Tabin, ibid., p. 1165; M. Hynes et al., Neuron 15, 35 (1995); S. C. Ekker et al., Development 121, 2337 (1995); R. Macdonald et al., ibid., p. 3267; S. C. Ekker et al., Curr. Biol. 5, 944 (1995); C.-J. Lai, S. C. Ekker, P. A. Beachy, R. T. Moon, Development 121, 2349 (1995); J. Ericson et al., Cell 81, 747 (1995).
    • (1993) Nature , vol.366 , pp. 560
    • Ingham, P.W.1
  • 7
    • 0026474841 scopus 로고
    • J. Mohler, Genetics 120, 1061 (1988); J. J. Lee, D. P. von Kessler, S. Parks, P. A. Beachy, Cell 71, 33 (1992); T. Tabata, S. Eaton, T. B. Kornberg, Genes Dev. 6, 2635 (1992); S. Tashiro et al., Gene 124, 183 (1993); P. W. Ingham, Nature 366, 560 (1993); J. Mohler and K. Vani, Development 115, 957 (1992); C. Ma, Y. Zhou, P. A. Beachy, K. Moses, Cell 75, 927 (1993); U. Heberlein, T. Wolff, G. Rubin, ibid., p. 913; Y. Echelard et al., ibid., p. 1417; R. D. Riddle, R. L. Johnson, E. Laufer, C. Tabin, ibid., p. 1401; S. Krauss, J.-P. Concordet, P. W. Ingham, ibid., p. 1431; H. Roelink et al., ibid. 76, 761 (1994); D. T. Chang et al., Development 120, 3339 (1994); K. Basler and G. Struhl, Nature 368, 208 (1994); T. Tabata and T. B. Komberg, Cell 76, 89 (1994); J. Heemskerkand S. DiNardo, ibid., p. 449; C.-M. Fan and M. Tessier-Lavigne, ibid. 79, 1175 (1994); R. L. Johnson, E. Laufer, R. D. Riddle, C. Tabin, ibid., p. 1165; M. Hynes et al., Neuron 15, 35 (1995); S. C. Ekker et al., Development 121, 2337 (1995); R. Macdonald et al., ibid., p. 3267; S. C. Ekker et al., Curr. Biol. 5, 944 (1995); C.-J. Lai, S. C. Ekker, P. A. Beachy, R. T. Moon, Development 121, 2349 (1995); J. Ericson et al., Cell 81, 747 (1995).
    • (1992) Development , vol.115 , pp. 957
    • Mohler, J.1    Vani, K.2
  • 8
    • 0027741258 scopus 로고
    • J. Mohler, Genetics 120, 1061 (1988); J. J. Lee, D. P. von Kessler, S. Parks, P. A. Beachy, Cell 71, 33 (1992); T. Tabata, S. Eaton, T. B. Kornberg, Genes Dev. 6, 2635 (1992); S. Tashiro et al., Gene 124, 183 (1993); P. W. Ingham, Nature 366, 560 (1993); J. Mohler and K. Vani, Development 115, 957 (1992); C. Ma, Y. Zhou, P. A. Beachy, K. Moses, Cell 75, 927 (1993); U. Heberlein, T. Wolff, G. Rubin, ibid., p. 913; Y. Echelard et al., ibid., p. 1417; R. D. Riddle, R. L. Johnson, E. Laufer, C. Tabin, ibid., p. 1401; S. Krauss, J.-P. Concordet, P. W. Ingham, ibid., p. 1431; H. Roelink et al., ibid. 76, 761 (1994); D. T. Chang et al., Development 120, 3339 (1994); K. Basler and G. Struhl, Nature 368, 208 (1994); T. Tabata and T. B. Komberg, Cell 76, 89 (1994); J. Heemskerkand S. DiNardo, ibid., p. 449; C.-M. Fan and M. Tessier-Lavigne, ibid. 79, 1175 (1994); R. L. Johnson, E. Laufer, R. D. Riddle, C. Tabin, ibid., p. 1165; M. Hynes et al., Neuron 15, 35 (1995); S. C. Ekker et al., Development 121, 2337 (1995); R. Macdonald et al., ibid., p. 3267; S. C. Ekker et al., Curr. Biol. 5, 944 (1995); C.-J. Lai, S. C. Ekker, P. A. Beachy, R. T. Moon, Development 121, 2349 (1995); J. Ericson et al., Cell 81, 747 (1995).
    • (1993) Cell , vol.75 , pp. 927
    • Ma, C.1    Zhou, Y.2    Beachy, P.A.3    Moses, K.4
  • 9
    • 0024254534 scopus 로고    scopus 로고
    • J. Mohler, Genetics 120, 1061 (1988); J. J. Lee, D. P. von Kessler, S. Parks, P. A. Beachy, Cell 71, 33 (1992); T. Tabata, S. Eaton, T. B. Kornberg, Genes Dev. 6, 2635 (1992); S. Tashiro et al., Gene 124, 183 (1993); P. W. Ingham, Nature 366, 560 (1993); J. Mohler and K. Vani, Development 115, 957 (1992); C. Ma, Y. Zhou, P. A. Beachy, K. Moses, Cell 75, 927 (1993); U. Heberlein, T. Wolff, G. Rubin, ibid., p. 913; Y. Echelard et al., ibid., p. 1417; R. D. Riddle, R. L. Johnson, E. Laufer, C. Tabin, ibid., p. 1401; S. Krauss, J.-P. Concordet, P. W. Ingham, ibid., p. 1431; H. Roelink et al., ibid. 76, 761 (1994); D. T. Chang et al., Development 120, 3339 (1994); K. Basler and G. Struhl, Nature 368, 208 (1994); T. Tabata and T. B. Komberg, Cell 76, 89 (1994); J. Heemskerkand S. DiNardo, ibid., p. 449; C.-M. Fan and M. Tessier-Lavigne, ibid. 79, 1175 (1994); R. L. Johnson, E. Laufer, R. D. Riddle, C. Tabin, ibid., p. 1165; M. Hynes et al., Neuron 15, 35 (1995); S. C. Ekker et al., Development 121, 2337 (1995); R. Macdonald et al., ibid., p. 3267; S. C. Ekker et al., Curr. Biol. 5, 944 (1995); C.-J. Lai, S. C. Ekker, P. A. Beachy, R. T. Moon, Development 121, 2349 (1995); J. Ericson et al., Cell 81, 747 (1995).
    • Cell , pp. 913
    • Heberlein, U.1    Wolff, T.2    Rubin, G.3
  • 10
    • 0024254534 scopus 로고    scopus 로고
    • J. Mohler, Genetics 120, 1061 (1988); J. J. Lee, D. P. von Kessler, S. Parks, P. A. Beachy, Cell 71, 33 (1992); T. Tabata, S. Eaton, T. B. Kornberg, Genes Dev. 6, 2635 (1992); S. Tashiro et al., Gene 124, 183 (1993); P. W. Ingham, Nature 366, 560 (1993); J. Mohler and K. Vani, Development 115, 957 (1992); C. Ma, Y. Zhou, P. A. Beachy, K. Moses, Cell 75, 927 (1993); U. Heberlein, T. Wolff, G. Rubin, ibid., p. 913; Y. Echelard et al., ibid., p. 1417; R. D. Riddle, R. L. Johnson, E. Laufer, C. Tabin, ibid., p. 1401; S. Krauss, J.-P. Concordet, P. W. Ingham, ibid., p. 1431; H. Roelink et al., ibid. 76, 761 (1994); D. T. Chang et al., Development 120, 3339 (1994); K. Basler and G. Struhl, Nature 368, 208 (1994); T. Tabata and T. B. Komberg, Cell 76, 89 (1994); J. Heemskerkand S. DiNardo, ibid., p. 449; C.-M. Fan and M. Tessier-Lavigne, ibid. 79, 1175 (1994); R. L. Johnson, E. Laufer, R. D. Riddle, C. Tabin, ibid., p. 1165; M. Hynes et al., Neuron 15, 35 (1995); S. C. Ekker et al., Development 121, 2337 (1995); R. Macdonald et al., ibid., p. 3267; S. C. Ekker et al., Curr. Biol. 5, 944 (1995); C.-J. Lai, S. C. Ekker, P. A. Beachy, R. T. Moon, Development 121, 2349 (1995); J. Ericson et al., Cell 81, 747 (1995).
    • Cell , pp. 1417
    • Echelard, Y.1
  • 11
    • 0024254534 scopus 로고    scopus 로고
    • J. Mohler, Genetics 120, 1061 (1988); J. J. Lee, D. P. von Kessler, S. Parks, P. A. Beachy, Cell 71, 33 (1992); T. Tabata, S. Eaton, T. B. Kornberg, Genes Dev. 6, 2635 (1992); S. Tashiro et al., Gene 124, 183 (1993); P. W. Ingham, Nature 366, 560 (1993); J. Mohler and K. Vani, Development 115, 957 (1992); C. Ma, Y. Zhou, P. A. Beachy, K. Moses, Cell 75, 927 (1993); U. Heberlein, T. Wolff, G. Rubin, ibid., p. 913; Y. Echelard et al., ibid., p. 1417; R. D. Riddle, R. L. Johnson, E. Laufer, C. Tabin, ibid., p. 1401; S. Krauss, J.-P. Concordet, P. W. Ingham, ibid., p. 1431; H. Roelink et al., ibid. 76, 761 (1994); D. T. Chang et al., Development 120, 3339 (1994); K. Basler and G. Struhl, Nature 368, 208 (1994); T. Tabata and T. B. Komberg, Cell 76, 89 (1994); J. Heemskerkand S. DiNardo, ibid., p. 449; C.-M. Fan and M. Tessier-Lavigne, ibid. 79, 1175 (1994); R. L. Johnson, E. Laufer, R. D. Riddle, C. Tabin, ibid., p. 1165; M. Hynes et al., Neuron 15, 35 (1995); S. C. Ekker et al., Development 121, 2337 (1995); R. Macdonald et al., ibid., p. 3267; S. C. Ekker et al., Curr. Biol. 5, 944 (1995); C.-J. Lai, S. C. Ekker, P. A. Beachy, R. T. Moon, Development 121, 2349 (1995); J. Ericson et al., Cell 81, 747 (1995).
    • Cell , pp. 1401
    • Riddle, R.D.1    Johnson, R.L.2    Laufer, E.3    Tabin, C.4
  • 12
    • 0024254534 scopus 로고    scopus 로고
    • J. Mohler, Genetics 120, 1061 (1988); J. J. Lee, D. P. von Kessler, S. Parks, P. A. Beachy, Cell 71, 33 (1992); T. Tabata, S. Eaton, T. B. Kornberg, Genes Dev. 6, 2635 (1992); S. Tashiro et al., Gene 124, 183 (1993); P. W. Ingham, Nature 366, 560 (1993); J. Mohler and K. Vani, Development 115, 957 (1992); C. Ma, Y. Zhou, P. A. Beachy, K. Moses, Cell 75, 927 (1993); U. Heberlein, T. Wolff, G. Rubin, ibid., p. 913; Y. Echelard et al., ibid., p. 1417; R. D. Riddle, R. L. Johnson, E. Laufer, C. Tabin, ibid., p. 1401; S. Krauss, J.-P. Concordet, P. W. Ingham, ibid., p. 1431; H. Roelink et al., ibid. 76, 761 (1994); D. T. Chang et al., Development 120, 3339 (1994); K. Basler and G. Struhl, Nature 368, 208 (1994); T. Tabata and T. B. Komberg, Cell 76, 89 (1994); J. Heemskerkand S. DiNardo, ibid., p. 449; C.-M. Fan and M. Tessier-Lavigne, ibid. 79, 1175 (1994); R. L. Johnson, E. Laufer, R. D. Riddle, C. Tabin, ibid., p. 1165; M. Hynes et al., Neuron 15, 35 (1995); S. C. Ekker et al., Development 121, 2337 (1995); R. Macdonald et al., ibid., p. 3267; S. C. Ekker et al., Curr. Biol. 5, 944 (1995); C.-J. Lai, S. C. Ekker, P. A. Beachy, R. T. Moon, Development 121, 2349 (1995); J. Ericson et al., Cell 81, 747 (1995).
    • Cell , pp. 1431
    • Krauss, S.1    Concordet, J.-P.2    Ingham, P.W.3
  • 13
    • 23444440157 scopus 로고
    • J. Mohler, Genetics 120, 1061 (1988); J. J. Lee, D. P. von Kessler, S. Parks, P. A. Beachy, Cell 71, 33 (1992); T. Tabata, S. Eaton, T. B. Kornberg, Genes Dev. 6, 2635 (1992); S. Tashiro et al., Gene 124, 183 (1993); P. W. Ingham, Nature 366, 560 (1993); J. Mohler and K. Vani, Development 115, 957 (1992); C. Ma, Y. Zhou, P. A. Beachy, K. Moses, Cell 75, 927 (1993); U. Heberlein, T. Wolff, G. Rubin, ibid., p. 913; Y. Echelard et al., ibid., p. 1417; R. D. Riddle, R. L. Johnson, E. Laufer, C. Tabin, ibid., p. 1401; S. Krauss, J.-P. Concordet, P. W. Ingham, ibid., p. 1431; H. Roelink et al., ibid. 76, 761 (1994); D. T. Chang et al., Development 120, 3339 (1994); K. Basler and G. Struhl, Nature 368, 208 (1994); T. Tabata and T. B. Komberg, Cell 76, 89 (1994); J. Heemskerkand S. DiNardo, ibid., p. 449; C.-M. Fan and M. Tessier-Lavigne, ibid. 79, 1175 (1994); R. L. Johnson, E. Laufer, R. D. Riddle, C. Tabin, ibid., p. 1165; M. Hynes et al., Neuron 15, 35 (1995); S. C. Ekker et al., Development 121, 2337 (1995); R. Macdonald et al., ibid., p. 3267; S. C. Ekker et al., Curr. Biol. 5, 944 (1995); C.-J. Lai, S. C. Ekker, P. A. Beachy, R. T. Moon, Development 121, 2349 (1995); J. Ericson et al., Cell 81, 747 (1995).
    • (1994) Cell , vol.76 , pp. 761
    • Roelink, H.1
  • 14
    • 0027939318 scopus 로고
    • J. Mohler, Genetics 120, 1061 (1988); J. J. Lee, D. P. von Kessler, S. Parks, P. A. Beachy, Cell 71, 33 (1992); T. Tabata, S. Eaton, T. B. Kornberg, Genes Dev. 6, 2635 (1992); S. Tashiro et al., Gene 124, 183 (1993); P. W. Ingham, Nature 366, 560 (1993); J. Mohler and K. Vani, Development 115, 957 (1992); C. Ma, Y. Zhou, P. A. Beachy, K. Moses, Cell 75, 927 (1993); U. Heberlein, T. Wolff, G. Rubin, ibid., p. 913; Y. Echelard et al., ibid., p. 1417; R. D. Riddle, R. L. Johnson, E. Laufer, C. Tabin, ibid., p. 1401; S. Krauss, J.-P. Concordet, P. W. Ingham, ibid., p. 1431; H. Roelink et al., ibid. 76, 761 (1994); D. T. Chang et al., Development 120, 3339 (1994); K. Basler and G. Struhl, Nature 368, 208 (1994); T. Tabata and T. B. Komberg, Cell 76, 89 (1994); J. Heemskerkand S. DiNardo, ibid., p. 449; C.-M. Fan and M. Tessier-Lavigne, ibid. 79, 1175 (1994); R. L. Johnson, E. Laufer, R. D. Riddle, C. Tabin, ibid., p. 1165; M. Hynes et al., Neuron 15, 35 (1995); S. C. Ekker et al., Development 121, 2337 (1995); R. Macdonald et al., ibid., p. 3267; S. C. Ekker et al., Curr. Biol. 5, 944 (1995); C.-J. Lai, S. C. Ekker, P. A. Beachy, R. T. Moon, Development 121, 2349 (1995); J. Ericson et al., Cell 81, 747 (1995).
    • (1994) Development , vol.120 , pp. 3339
    • Chang, D.T.1
  • 15
    • 0028329349 scopus 로고
    • J. Mohler, Genetics 120, 1061 (1988); J. J. Lee, D. P. von Kessler, S. Parks, P. A. Beachy, Cell 71, 33 (1992); T. Tabata, S. Eaton, T. B. Kornberg, Genes Dev. 6, 2635 (1992); S. Tashiro et al., Gene 124, 183 (1993); P. W. Ingham, Nature 366, 560 (1993); J. Mohler and K. Vani, Development 115, 957 (1992); C. Ma, Y. Zhou, P. A. Beachy, K. Moses, Cell 75, 927 (1993); U. Heberlein, T. Wolff, G. Rubin, ibid., p. 913; Y. Echelard et al., ibid., p. 1417; R. D. Riddle, R. L. Johnson, E. Laufer, C. Tabin, ibid., p. 1401; S. Krauss, J.-P. Concordet, P. W. Ingham, ibid., p. 1431; H. Roelink et al., ibid. 76, 761 (1994); D. T. Chang et al., Development 120, 3339 (1994); K. Basler and G. Struhl, Nature 368, 208 (1994); T. Tabata and T. B. Komberg, Cell 76, 89 (1994); J. Heemskerkand S. DiNardo, ibid., p. 449; C.-M. Fan and M. Tessier-Lavigne, ibid. 79, 1175 (1994); R. L. Johnson, E. Laufer, R. D. Riddle, C. Tabin, ibid., p. 1165; M. Hynes et al., Neuron 15, 35 (1995); S. C. Ekker et al., Development 121, 2337 (1995); R. Macdonald et al., ibid., p. 3267; S. C. Ekker et al., Curr. Biol. 5, 944 (1995); C.-J. Lai, S. C. Ekker, P. A. Beachy, R. T. Moon, Development 121, 2349 (1995); J. Ericson et al., Cell 81, 747 (1995).
    • (1994) Nature , vol.368 , pp. 208
    • Basler, K.1    Struhl, G.2
  • 16
    • 0028178786 scopus 로고
    • J. Mohler, Genetics 120, 1061 (1988); J. J. Lee, D. P. von Kessler, S. Parks, P. A. Beachy, Cell 71, 33 (1992); T. Tabata, S. Eaton, T. B. Kornberg, Genes Dev. 6, 2635 (1992); S. Tashiro et al., Gene 124, 183 (1993); P. W. Ingham, Nature 366, 560 (1993); J. Mohler and K. Vani, Development 115, 957 (1992); C. Ma, Y. Zhou, P. A. Beachy, K. Moses, Cell 75, 927 (1993); U. Heberlein, T. Wolff, G. Rubin, ibid., p. 913; Y. Echelard et al., ibid., p. 1417; R. D. Riddle, R. L. Johnson, E. Laufer, C. Tabin, ibid., p. 1401; S. Krauss, J.-P. Concordet, P. W. Ingham, ibid., p. 1431; H. Roelink et al., ibid. 76, 761 (1994); D. T. Chang et al., Development 120, 3339 (1994); K. Basler and G. Struhl, Nature 368, 208 (1994); T. Tabata and T. B. Komberg, Cell 76, 89 (1994); J. Heemskerkand S. DiNardo, ibid., p. 449; C.-M. Fan and M. Tessier-Lavigne, ibid. 79, 1175 (1994); R. L. Johnson, E. Laufer, R. D. Riddle, C. Tabin, ibid., p. 1165; M. Hynes et al., Neuron 15, 35 (1995); S. C. Ekker et al., Development 121, 2337 (1995); R. Macdonald et al., ibid., p. 3267; S. C. Ekker et al., Curr. Biol. 5, 944 (1995); C.-J. Lai, S. C. Ekker, P. A. Beachy, R. T. Moon, Development 121, 2349 (1995); J. Ericson et al., Cell 81, 747 (1995).
    • (1994) Cell , vol.76 , pp. 89
    • Tabata, T.1    Komberg, T.B.2
  • 17
    • 0024254534 scopus 로고    scopus 로고
    • J. Mohler, Genetics 120, 1061 (1988); J. J. Lee, D. P. von Kessler, S. Parks, P. A. Beachy, Cell 71, 33 (1992); T. Tabata, S. Eaton, T. B. Kornberg, Genes Dev. 6, 2635 (1992); S. Tashiro et al., Gene 124, 183 (1993); P. W. Ingham, Nature 366, 560 (1993); J. Mohler and K. Vani, Development 115, 957 (1992); C. Ma, Y. Zhou, P. A. Beachy, K. Moses, Cell 75, 927 (1993); U. Heberlein, T. Wolff, G. Rubin, ibid., p. 913; Y. Echelard et al., ibid., p. 1417; R. D. Riddle, R. L. Johnson, E. Laufer, C. Tabin, ibid., p. 1401; S. Krauss, J.-P. Concordet, P. W. Ingham, ibid., p. 1431; H. Roelink et al., ibid. 76, 761 (1994); D. T. Chang et al., Development 120, 3339 (1994); K. Basler and G. Struhl, Nature 368, 208 (1994); T. Tabata and T. B. Komberg, Cell 76, 89 (1994); J. Heemskerkand S. DiNardo, ibid., p. 449; C.-M. Fan and M. Tessier-Lavigne, ibid. 79, 1175 (1994); R. L. Johnson, E. Laufer, R. D. Riddle, C. Tabin, ibid., p. 1165; M. Hynes et al., Neuron 15, 35 (1995); S. C. Ekker et al., Development 121, 2337 (1995); R. Macdonald et al., ibid., p. 3267; S. C. Ekker et al., Curr. Biol. 5, 944 (1995); C.-J. Lai, S. C. Ekker, P. A. Beachy, R. T. Moon, Development 121, 2349 (1995); J. Ericson et al., Cell 81, 747 (1995).
    • Cell , pp. 449
    • Heemskerk, J.1    DiNardo, S.2
  • 18
    • 0028630689 scopus 로고
    • J. Mohler, Genetics 120, 1061 (1988); J. J. Lee, D. P. von Kessler, S. Parks, P. A. Beachy, Cell 71, 33 (1992); T. Tabata, S. Eaton, T. B. Kornberg, Genes Dev. 6, 2635 (1992); S. Tashiro et al., Gene 124, 183 (1993); P. W. Ingham, Nature 366, 560 (1993); J. Mohler and K. Vani, Development 115, 957 (1992); C. Ma, Y. Zhou, P. A. Beachy, K. Moses, Cell 75, 927 (1993); U. Heberlein, T. Wolff, G. Rubin, ibid., p. 913; Y. Echelard et al., ibid., p. 1417; R. D. Riddle, R. L. Johnson, E. Laufer, C. Tabin, ibid., p. 1401; S. Krauss, J.-P. Concordet, P. W. Ingham, ibid., p. 1431; H. Roelink et al., ibid. 76, 761 (1994); D. T. Chang et al., Development 120, 3339 (1994); K. Basler and G. Struhl, Nature 368, 208 (1994); T. Tabata and T. B. Komberg, Cell 76, 89 (1994); J. Heemskerkand S. DiNardo, ibid., p. 449; C.-M. Fan and M. Tessier-Lavigne, ibid. 79, 1175 (1994); R. L. Johnson, E. Laufer, R. D. Riddle, C. Tabin, ibid., p. 1165; M. Hynes et al., Neuron 15, 35 (1995); S. C. Ekker et al., Development 121, 2337 (1995); R. Macdonald et al., ibid., p. 3267; S. C. Ekker et al., Curr. Biol. 5, 944 (1995); C.-J. Lai, S. C. Ekker, P. A. Beachy, R. T. Moon, Development 121, 2349 (1995); J. Ericson et al., Cell 81, 747 (1995).
    • (1994) Cell , vol.79 , pp. 1175
    • Fan, C.-M.1    Tessier-Lavigne, M.2
  • 19
    • 0024254534 scopus 로고    scopus 로고
    • J. Mohler, Genetics 120, 1061 (1988); J. J. Lee, D. P. von Kessler, S. Parks, P. A. Beachy, Cell 71, 33 (1992); T. Tabata, S. Eaton, T. B. Kornberg, Genes Dev. 6, 2635 (1992); S. Tashiro et al., Gene 124, 183 (1993); P. W. Ingham, Nature 366, 560 (1993); J. Mohler and K. Vani, Development 115, 957 (1992); C. Ma, Y. Zhou, P. A. Beachy, K. Moses, Cell 75, 927 (1993); U. Heberlein, T. Wolff, G. Rubin, ibid., p. 913; Y. Echelard et al., ibid., p. 1417; R. D. Riddle, R. L. Johnson, E. Laufer, C. Tabin, ibid., p. 1401; S. Krauss, J.-P. Concordet, P. W. Ingham, ibid., p. 1431; H. Roelink et al., ibid. 76, 761 (1994); D. T. Chang et al., Development 120, 3339 (1994); K. Basler and G. Struhl, Nature 368, 208 (1994); T. Tabata and T. B. Komberg, Cell 76, 89 (1994); J. Heemskerkand S. DiNardo, ibid., p. 449; C.-M. Fan and M. Tessier-Lavigne, ibid. 79, 1175 (1994); R. L. Johnson, E. Laufer, R. D. Riddle, C. Tabin, ibid., p. 1165; M. Hynes et al., Neuron 15, 35 (1995); S. C. Ekker et al., Development 121, 2337 (1995); R. Macdonald et al., ibid., p. 3267; S. C. Ekker et al., Curr. Biol. 5, 944 (1995); C.-J. Lai, S. C. Ekker, P. A. Beachy, R. T. Moon, Development 121, 2349 (1995); J. Ericson et al., Cell 81, 747 (1995).
    • Cell , pp. 1165
    • Johnson, R.L.1    Laufer, E.2    Riddle, R.D.3    Tabin, C.4
  • 20
    • 0029127630 scopus 로고
    • J. Mohler, Genetics 120, 1061 (1988); J. J. Lee, D. P. von Kessler, S. Parks, P. A. Beachy, Cell 71, 33 (1992); T. Tabata, S. Eaton, T. B. Kornberg, Genes Dev. 6, 2635 (1992); S. Tashiro et al., Gene 124, 183 (1993); P. W. Ingham, Nature 366, 560 (1993); J. Mohler and K. Vani, Development 115, 957 (1992); C. Ma, Y. Zhou, P. A. Beachy, K. Moses, Cell 75, 927 (1993); U. Heberlein, T. Wolff, G. Rubin, ibid., p. 913; Y. Echelard et al., ibid., p. 1417; R. D. Riddle, R. L. Johnson, E. Laufer, C. Tabin, ibid., p. 1401; S. Krauss, J.-P. Concordet, P. W. Ingham, ibid., p. 1431; H. Roelink et al., ibid. 76, 761 (1994); D. T. Chang et al., Development 120, 3339 (1994); K. Basler and G. Struhl, Nature 368, 208 (1994); T. Tabata and T. B. Komberg, Cell 76, 89 (1994); J. Heemskerkand S. DiNardo, ibid., p. 449; C.-M. Fan and M. Tessier-Lavigne, ibid. 79, 1175 (1994); R. L. Johnson, E. Laufer, R. D. Riddle, C. Tabin, ibid., p. 1165; M. Hynes et al., Neuron 15, 35 (1995); S. C. Ekker et al., Development 121, 2337 (1995); R. Macdonald et al., ibid., p. 3267; S. C. Ekker et al., Curr. Biol. 5, 944 (1995); C.-J. Lai, S. C. Ekker, P. A. Beachy, R. T. Moon, Development 121, 2349 (1995); J. Ericson et al., Cell 81, 747 (1995).
    • (1995) Neuron , vol.15 , pp. 35
    • Hynes, M.1
  • 21
    • 0029024046 scopus 로고
    • J. Mohler, Genetics 120, 1061 (1988); J. J. Lee, D. P. von Kessler, S. Parks, P. A. Beachy, Cell 71, 33 (1992); T. Tabata, S. Eaton, T. B. Kornberg, Genes Dev. 6, 2635 (1992); S. Tashiro et al., Gene 124, 183 (1993); P. W. Ingham, Nature 366, 560 (1993); J. Mohler and K. Vani, Development 115, 957 (1992); C. Ma, Y. Zhou, P. A. Beachy, K. Moses, Cell 75, 927 (1993); U. Heberlein, T. Wolff, G. Rubin, ibid., p. 913; Y. Echelard et al., ibid., p. 1417; R. D. Riddle, R. L. Johnson, E. Laufer, C. Tabin, ibid., p. 1401; S. Krauss, J.-P. Concordet, P. W. Ingham, ibid., p. 1431; H. Roelink et al., ibid. 76, 761 (1994); D. T. Chang et al., Development 120, 3339 (1994); K. Basler and G. Struhl, Nature 368, 208 (1994); T. Tabata and T. B. Komberg, Cell 76, 89 (1994); J. Heemskerkand S. DiNardo, ibid., p. 449; C.-M. Fan and M. Tessier-Lavigne, ibid. 79, 1175 (1994); R. L. Johnson, E. Laufer, R. D. Riddle, C. Tabin, ibid., p. 1165; M. Hynes et al., Neuron 15, 35 (1995); S. C. Ekker et al., Development 121, 2337 (1995); R. Macdonald et al., ibid., p. 3267; S. C. Ekker et al., Curr. Biol. 5, 944 (1995); C.-J. Lai, S. C. Ekker, P. A. Beachy, R. T. Moon, Development 121, 2349 (1995); J. Ericson et al., Cell 81, 747 (1995).
    • (1995) Development , vol.121 , pp. 2337
    • Ekker, S.C.1
  • 22
    • 0024254534 scopus 로고    scopus 로고
    • J. Mohler, Genetics 120, 1061 (1988); J. J. Lee, D. P. von Kessler, S. Parks, P. A. Beachy, Cell 71, 33 (1992); T. Tabata, S. Eaton, T. B. Kornberg, Genes Dev. 6, 2635 (1992); S. Tashiro et al., Gene 124, 183 (1993); P. W. Ingham, Nature 366, 560 (1993); J. Mohler and K. Vani, Development 115, 957 (1992); C. Ma, Y. Zhou, P. A. Beachy, K. Moses, Cell 75, 927 (1993); U. Heberlein, T. Wolff, G. Rubin, ibid., p. 913; Y. Echelard et al., ibid., p. 1417; R. D. Riddle, R. L. Johnson, E. Laufer, C. Tabin, ibid., p. 1401; S. Krauss, J.-P. Concordet, P. W. Ingham, ibid., p. 1431; H. Roelink et al., ibid. 76, 761 (1994); D. T. Chang et al., Development 120, 3339 (1994); K. Basler and G. Struhl, Nature 368, 208 (1994); T. Tabata and T. B. Komberg, Cell 76, 89 (1994); J. Heemskerkand S. DiNardo, ibid., p. 449; C.-M. Fan and M. Tessier-Lavigne, ibid. 79, 1175 (1994); R. L. Johnson, E. Laufer, R. D. Riddle, C. Tabin, ibid., p. 1165; M. Hynes et al., Neuron 15, 35 (1995); S. C. Ekker et al., Development 121, 2337 (1995); R. Macdonald et al., ibid., p. 3267; S. C. Ekker et al., Curr. Biol. 5, 944 (1995); C.-J. Lai, S. C. Ekker, P. A. Beachy, R. T. Moon, Development 121, 2349 (1995); J. Ericson et al., Cell 81, 747 (1995).
    • Development , pp. 3267
    • Macdonald, R.1
  • 23
    • 0029347087 scopus 로고
    • J. Mohler, Genetics 120, 1061 (1988); J. J. Lee, D. P. von Kessler, S. Parks, P. A. Beachy, Cell 71, 33 (1992); T. Tabata, S. Eaton, T. B. Kornberg, Genes Dev. 6, 2635 (1992); S. Tashiro et al., Gene 124, 183 (1993); P. W. Ingham, Nature 366, 560 (1993); J. Mohler and K. Vani, Development 115, 957 (1992); C. Ma, Y. Zhou, P. A. Beachy, K. Moses, Cell 75, 927 (1993); U. Heberlein, T. Wolff, G. Rubin, ibid., p. 913; Y. Echelard et al., ibid., p. 1417; R. D. Riddle, R. L. Johnson, E. Laufer, C. Tabin, ibid., p. 1401; S. Krauss, J.-P. Concordet, P. W. Ingham, ibid., p. 1431; H. Roelink et al., ibid. 76, 761 (1994); D. T. Chang et al., Development 120, 3339 (1994); K. Basler and G. Struhl, Nature 368, 208 (1994); T. Tabata and T. B. Komberg, Cell 76, 89 (1994); J. Heemskerkand S. DiNardo, ibid., p. 449; C.-M. Fan and M. Tessier-Lavigne, ibid. 79, 1175 (1994); R. L. Johnson, E. Laufer, R. D. Riddle, C. Tabin, ibid., p. 1165; M. Hynes et al., Neuron 15, 35 (1995); S. C. Ekker et al., Development 121, 2337 (1995); R. Macdonald et al., ibid., p. 3267; S. C. Ekker et al., Curr. Biol. 5, 944 (1995); C.-J. Lai, S. C. Ekker, P. A. Beachy, R. T. Moon, Development 121, 2349 (1995); J. Ericson et al., Cell 81, 747 (1995).
    • (1995) Curr. Biol. , vol.5 , pp. 944
    • Ekker, S.C.1
  • 24
    • 0029028990 scopus 로고
    • J. Mohler, Genetics 120, 1061 (1988); J. J. Lee, D. P. von Kessler, S. Parks, P. A. Beachy, Cell 71, 33 (1992); T. Tabata, S. Eaton, T. B. Kornberg, Genes Dev. 6, 2635 (1992); S. Tashiro et al., Gene 124, 183 (1993); P. W. Ingham, Nature 366, 560 (1993); J. Mohler and K. Vani, Development 115, 957 (1992); C. Ma, Y. Zhou, P. A. Beachy, K. Moses, Cell 75, 927 (1993); U. Heberlein, T. Wolff, G. Rubin, ibid., p. 913; Y. Echelard et al., ibid., p. 1417; R. D. Riddle, R. L. Johnson, E. Laufer, C. Tabin, ibid., p. 1401; S. Krauss, J.-P. Concordet, P. W. Ingham, ibid., p. 1431; H. Roelink et al., ibid. 76, 761 (1994); D. T. Chang et al., Development 120, 3339 (1994); K. Basler and G. Struhl, Nature 368, 208 (1994); T. Tabata and T. B. Komberg, Cell 76, 89 (1994); J. Heemskerkand S. DiNardo, ibid., p. 449; C.-M. Fan and M. Tessier-Lavigne, ibid. 79, 1175 (1994); R. L. Johnson, E. Laufer, R. D. Riddle, C. Tabin, ibid., p. 1165; M. Hynes et al., Neuron 15, 35 (1995); S. C. Ekker et al., Development 121, 2337 (1995); R. Macdonald et al., ibid., p. 3267; S. C. Ekker et al., Curr. Biol. 5, 944 (1995); C.-J. Lai, S. C. Ekker, P. A. Beachy, R. T. Moon, Development 121, 2349 (1995); J. Ericson et al., Cell 81, 747 (1995).
    • (1995) Development , vol.121 , pp. 2349
    • Lai, C.-J.1    Ekker, S.C.2    Beachy, P.A.3    Moon, R.T.4
  • 25
    • 0029068123 scopus 로고
    • J. Mohler, Genetics 120, 1061 (1988); J. J. Lee, D. P. von Kessler, S. Parks, P. A. Beachy, Cell 71, 33 (1992); T. Tabata, S. Eaton, T. B. Kornberg, Genes Dev. 6, 2635 (1992); S. Tashiro et al., Gene 124, 183 (1993); P. W. Ingham, Nature 366, 560 (1993); J. Mohler and K. Vani, Development 115, 957 (1992); C. Ma, Y. Zhou, P. A. Beachy, K. Moses, Cell 75, 927 (1993); U. Heberlein, T. Wolff, G. Rubin, ibid., p. 913; Y. Echelard et al., ibid., p. 1417; R. D. Riddle, R. L. Johnson, E. Laufer, C. Tabin, ibid., p. 1401; S. Krauss, J.-P. Concordet, P. W. Ingham, ibid., p. 1431; H. Roelink et al., ibid. 76, 761 (1994); D. T. Chang et al., Development 120, 3339 (1994); K. Basler and G. Struhl, Nature 368, 208 (1994); T. Tabata and T. B. Komberg, Cell 76, 89 (1994); J. Heemskerkand S. DiNardo, ibid., p. 449; C.-M. Fan and M. Tessier-Lavigne, ibid. 79, 1175 (1994); R. L. Johnson, E. Laufer, R. D. Riddle, C. Tabin, ibid., p. 1165; M. Hynes et al., Neuron 15, 35 (1995); S. C. Ekker et al., Development 121, 2337 (1995); R. Macdonald et al., ibid., p. 3267; S. C. Ekker et al., Curr. Biol. 5, 944 (1995); C.-J. Lai, S. C. Ekker, P. A. Beachy, R. T. Moon, Development 121, 2349 (1995); J. Ericson et al., Cell 81, 747 (1995).
    • (1995) Cell , vol.81 , pp. 747
    • Ericson, J.1
  • 26
    • 0029777408 scopus 로고    scopus 로고
    • C. Chiang et al., Nature 83, 407 (1996).
    • (1996) Nature , vol.83 , pp. 407
    • Chiang, C.1
  • 28
    • 0029750190 scopus 로고    scopus 로고
    • A. Vortkamp et al., Science 273, 613 (1996).
    • (1996) Science , vol.273 , pp. 613
    • Vortkamp, A.1
  • 29
    • 0028641336 scopus 로고
    • J. J. Lee et al., ibid. 266, 1528 (1994).
    • (1994) Science , vol.266 , pp. 1528
    • Lee, J.J.1
  • 30
    • 0028948811 scopus 로고
    • J. A. Porter et al., Nature 374, 363 (1995).
    • (1995) Nature , vol.374 , pp. 363
    • Porter, J.A.1
  • 31
    • 15844386540 scopus 로고    scopus 로고
    • J. A. Porter et al., Cell 86, 21 (1996).
    • (1996) Cell , vol.86 , pp. 21
    • Porter, J.A.1
  • 33
  • 36
    • 10244281161 scopus 로고    scopus 로고
    • J. A. Porter, K. E. Young, P. A. Beachy, data not shown
    • J. A. Porter, K. E. Young, P. A. Beachy, data not shown.
  • 44
    • 10244279909 scopus 로고    scopus 로고
    • note
    • 2O and reprecipitated with acetone several times to remove unincorporated radioactivity before loading onto SDS-polyacrylamide gels for analysis. Initial labeling experiments with 25 μCi of added cholesterol resulted in reduced incorporation by a factor of ∼10.
  • 45
    • 10244281160 scopus 로고    scopus 로고
    • note
    • 2O, and extraction of the hydrophobic radioactivity with ether. After evaporation of the ether, the sample was resuspended in isopropanol and applied to the C18 column for analysis (20).
  • 47
    • 10244276512 scopus 로고    scopus 로고
    • note
    • 4 dpm by excision and scintillation counting of an Hh-Np gel band.
  • 48
    • 0004234130 scopus 로고
    • Allyn Bacon, Boston, ed. 3
    • 2O across the 5,6 double bond of cholesterol, a common reaction of secondary alkenes in strong acids [R. T. Morrison and R. N. Boyd, Organic Chemistry (Allyn Bacon, Boston, ed. 3, 1973)], followed by estenfication of formate via this newly formed alcohol [B. I. Cohen, G. S. Tint, T.Kuramoto, E. H. Mosbach, Steroids 25, 365 (1975)]. To determine whether the sterol backbone could be modified by the CNBr treatment, we examined a positively charged cholesterol derivative (3β(N-(N',N'-dimethylamino)ethanecarbamoyl)-cholesterol) (Sigma) detectable by MALDI. Incubation of this sterd derivative in 70% formic acid alone resulted in the addition of 45 mass units to the sterol (13), a mass consistent with the net addition of a formic acid molecule.
    • (1973) Organic Chemistry
    • Morrison, R.T.1    Boyd, R.N.2
  • 49
    • 0016668742 scopus 로고
    • 2O across the 5,6 double bond of cholesterol, a common reaction of secondary alkenes in strong acids [R. T. Morrison and R. N. Boyd, Organic Chemistry (Allyn Bacon, Boston, ed. 3, 1973)], followed by estenfication of formate via this newly formed alcohol [B. I. Cohen, G. S. Tint, T.Kuramoto, E. H. Mosbach, Steroids 25, 365 (1975)]. To determine whether the sterol backbone could be modified by the CNBr treatment, we examined a positively charged cholesterol derivative (3β(N-(N',N'-dimethylamino)ethanecarbamoyl)-cholesterol) (Sigma) detectable by MALDI. Incubation of this sterd derivative in 70% formic acid alone resulted in the addition of 45 mass units to the sterol (13), a mass consistent with the net addition of a formic acid molecule.
    • (1975) Steroids , vol.25 , pp. 365
    • Cohen, B.I.1    Tint, G.S.2    Kuramoto, T.3    Mosbach, E.H.4
  • 50
    • 10244272420 scopus 로고    scopus 로고
    • note
    • 3H]cholesterol, giving a final concentration of cholesterol of ∼40 μg/ml with a specific activity of 2 Ci/mmol (labeling medium). After 24 hours the labeling medium was removed and the cells were transfected for 6 hours with Shh or Shh-N expression constructs with lipofectamine (Gibco) and serumfree DMEM. After transfection, 1 ml of fresh labeling medium was added to each dish, and the cells were incubated for 36 hours at 37°C. The cells were then harvested without washing and lysed on the plate with tris-buffered saline plus 1% Triton X-100, and the total cell proteins were precipitated with acetone, washed, and analyzed as described above for the S2 cell proteins.
  • 67
    • 0000275754 scopus 로고
    • N. M. Hooper and A. J. Turner, Eds. Oxford Univ. Press, New York
    • M. C. Field and A. K. Menon, in Lipid Modification of Proteins, N. M. Hooper and A. J. Turner, Eds. (Oxford Univ. Press, New York, 1992), p. 155.
    • (1992) Lipid Modification of Proteins , pp. 155
    • Field, M.C.1    Menon, A.K.2
  • 68
    • 10244272110 scopus 로고    scopus 로고
    • note
    • We are grateful to A. Woods and D. Raben for use of equipment and for helpful advice. We thank J. Nathans, D. Leahy, and T. Tanaka-Hall for comments on the manuscript. P.A.B. is an Investigator of the Howard Hughes Medical Institute.


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