Transcriptional regulation of the human hepatic CYP3A4: Identification of a new distal enhancer region responsive to CCAAT/enhancer-binding protein β isoforms (liver activating protein and liver inhibitory protein)

Molecular Pharmacology

Volumn 67, Issue 6, 2005, Pages 2088-2101

  Martínez Jiménez, Celia P ^a,b   Gómez Lechón, M José ^a   Castell, José V ^a,b   Jover, Ramiro ^a,b  

^a HOSPITAL UNIVERSITARIO LA FE   (Spain)

^b UNIVERSITY OF VALENCIA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOVIRUS VECTOR; BINDING PROTEIN; CCAAT BINDING FACTOR; CYTOCHROME P450 3A4; LUCIFERASE; CCAAT ENHANCER BINDING PROTEIN; CYP3A PROTEIN, HUMAN; CYP3A4 PROTEIN, HUMAN; CYTOCHROME P450; ISOPROTEIN; REPRESSOR PROTEIN; TRANSACTIVATOR PROTEIN;

ARTICLE; BASE PAIRING; BINDING SITE; CELL STRAIN HEPG2; CHROMATIN; CONTROLLED STUDY; CYTOKINE RELEASE; DNA FLANKING REGION; DOWN REGULATION; ENHANCER REGION; EXPRESSION VECTOR; GENE ACTIVITY; GENE CONTROL; GENE REPRESSION; GENE SEQUENCE; GENETIC TRANSCRIPTION; GENETIC TRANSFECTION; HELA CELL; HUMAN; HUMAN CELL; IMMUNOPRECIPITATION; LIVER CELL; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN MOTIF; SITE DIRECTED MUTAGENESIS; TRANSCRIPTION REGULATION; ANIMAL; CELL LINE; COMPARATIVE STUDY; DRUG ANTAGONISM; GENETICS; INSECT; LIVER; METABOLISM; MOLECULAR GENETICS; PHYSIOLOGY;

ANIMALS; BASE SEQUENCE; CCAAT-ENHANCER-BINDING PROTEINS; CELL LINE; CYTOCHROME P-450 ENZYME SYSTEM; HUMANS; INSECTS; LIVER; MOLECULAR SEQUENCE DATA; PROTEIN ISOFORMS; REPRESSOR PROTEINS; TRANS-ACTIVATORS; TRANSCRIPTION, GENETIC;

EID: 21744451043     PISSN: 0026895X     EISSN: None     Source Type: Journal    
DOI: 10.1124/mol.104.008169     Document Type: Article

References (52)

1. Akira S, Isshiki H, Sugita T, Tanabe O, Kinoshita S, Nishio Y, Nakajima T, Hirano T, and Kishimoto T (1990) A nuclear factor for IL-6 expression (NF-IL6) is a member of a C/EBP family. EMBO (Eur Mol Biol Organ) J 9:1897-1906.
2. An M, Hsieh C, Reisner P, Rabek J, Scott S, Kuninger D, and Papaconstantinou J (1996) Evidence for posttranscriptional regulation of C/EBPalpha and C/EBPbeta isoform expression during the lipopolysaccharide- mediated acute-phase response. Mol Cell Biol 16:2295-2306.
3. Andrews N and Faller D (1991) A rapid micropreparation technique for extraction of DNA-binding proteins from limiting numbers of mammalian cells. Nucleic Acids Res 19:2499.
4. Assenat E, Gerbal-Chaloin S, Larrey D, Saric J, Fabre JM, Maurel P, Vilarem MJ, and Pascussi JM (2004) Interleukin 1beta inhibits CAR-induced expression of hepatic genes involved in drug and bilirubin clearance. Hepatology 40:951-960.
5. Barnett CR, Gibson GG, Wolf CR, Flatt PR, and Ioannides C (1990) Induction of cytochrome P450III and P450IV family proteins in streptozotocin-induced diabetes. Biochem J 268:765-769.
6. Baumann H, Morella K, Campos S, Cao Z, and Jahreis G (1992) Role of CAAT-enhancer binding protein isoforms in the cytokine regulation of acute-phase plasma protein genes. J Biol Chem 267:19744-19751.
7. Bertilsson G, Heidrich J, Svensson K, Asman M, Jendeberg L, Sydow-Backman M, Ohlsson R, Postlind H, Blomquist P, and Berkenstam A (1998) Identification of a human nuclear receptor defines a new signaling pathway for CYP3A induction. Proc Natl Acad Sci USA 95:12208-12213.
8. Blumberg B, Sabbagh W Jr, Juguilon H, Bolado J Jr, van Meter CM, Ong ES, and Evans RM (1998) SXR, a novel steroid and xenobiotic-sensing nuclear receptor. Genes Dev 12:3195-3205.
9. Borras E, Zaragoza R, Morante M, Garcia C, Gimeno A, Lopez-Rodas G, Barber T, Miralles VJ, Vina JR, and Torres L (2003) In vivo studies of altered expression patterns of p53 and proliferative control genes in chronic vitamin A deficiency and hypervitaminosis. Eur J Biochem 270:1493-1501.
10. Bosch F, Sabater J, and Valera A (1995) Insulin inhibits liver expression of the CCAAT/enhancer-binding protein beta. Diabetes 44:267-271.
11. Castell JV, Hernandez D, Gomez-Foix AM, Guillen I, Donato T, and Gomez-Lechon MJ (1997) Adenovirus-mediated gene transfer into human hepatocytes: analysis of the biochemical functionality of transduced cells. Gene Ther 4:455-464.
12. Descombes P, Chojkier M, Lichtsteiner S, Falvey E, and Schibler U (1990) LAP, a novel member of the C/EBP gene family, encodes a liver-enriched transcriptional activator protein. Genes Dev 4:1541-1551.
13. Descombes P and Schibler U (1991) A liver-enriched transcriptional activator protein, LAP and a transcriptional inhibitory protein, LIP, are translated from the same mRNA. Cell 67:569-579.
14. Diehl A (1998) Roles of CCAAT/enhancer-binding proteins in regulation of liver regenerative growth. J Biol Chem 273:30843-30846.
15. Diehl AM and Yang SQ (1994) Regenerative changes in C/EBP alpha and C/EBP beta expression modulate binding to the C/EBP site in the c-fos promoter. Hepatology 19:447-456.
16. Drocourt L, Ourlin JC, Pascussi JM, Maurel P, and Vilarem MJ (2002) Expression of CYP3A4, CYP2B6 and CYP2C9 is regulated by the vitamin D receptor pathway in primary human hepatocytes. J Biol Chem 277:25125-25132.
17. Duong DT, Waltner-Law ME, Sears R, Sealy L, and Granner DK (2002) Insulin inhibits hepatocellular glucose production by utilizing liver-enriched transcriptional inhibitory protein to disrupt the association of CREB-binding protein and RNA polymerase II with the phosphoenolpyruvate carboxykinase gene promoter. J Biol Chem 277:32234-32242.
18. Friedman AD, Landschulz WH, and McKnight SL (1989) CCAAT/enhancer binding protein activates the promoter of the serum albumin gene in cultured hepatoma cells. Genes Dev 3:1314-1322.
19. Gomez-Lechon M and Castell J (2000) Isolation and culture of human hepatocytes, in The Hepatocyte Review (Berry MN and Edwards AM eds) pp 11-17, Kluwer Academic Publishers, London.
20. Goodwin B, Hodgson E, D'Costa DJ, Robertson GR, and Liddle C (2002) Transcriptional regulation of the human CYP3A4 gene by the constitutive androstane receptor. Mol Pharmacol 62:359-365.
21. Goodwin B, Hodgson E, and Liddle C (1999) The orphan human pregnane X receptor mediates the transcriptional activation of CYP3A4 by rifampicin through a distal enhancer module. Mol Pharmacol 56:1329-1339.
22. Guengerich FP (1999) Cytochrome P-450 3A4: regulation and role in drug metabolism. Annu Rev Pharmacol Toxicol 39:1-17.
23. Hsieh C, Xiong W, Xie Q, Rabek J, Scott S, An M, Reisner P, Kuninger D, and Papaconstantinou J (1998) Effects of age on the posttranscriptional regulation of CCAAT/enhancer binding protein alpha and CCAAT/enhancer binding protein beta isoform synthesis in control and LPS-treated livers. Mol Biol Cell 9:1479-1494.
24. Iber H, Chen Q, Cheng P, and Morgan E (2000) Suppression of CYP2C11 gene transcription by interleukin-1 mediated by NF-kappaB binding at the transcription start site. Arch Biochem Biophys 377:187-194.
25. Iraburu M, Dominguez-Rosales J, Fontana L, Auster A, Garcia-Trevijano E, Covarrubias-Pinedo A, Rivas-Estilla A, Greenwel P, and Rojkind M (2000) Tumor necrosis factor alpha down-regulates expression of the alpha1(I) collagen gene in rat hepatic stellate cells through a p20C/EBPbeta- and C/EBPdelta-dependent mechanism. Hepatology 31:1086-1093.
26. Jover R, Bort R, Gomez-Lechon MJ, and Castell JV (2001) Cytochrome P450 regulation by hepatocyte nuclear factor 4 in human hepatocytes: a study using adenovirus-mediated antisense targeting. Hepatology 33:668-675.
27. Jover R, Bort R, Gomez-Lechon MJ, and Castell JV (2002) Down-regulation of human CYP3A4 by the inflammatory signal interleukin-6: molecular mechanism and transcription factors involved. FASEB J 16:1799-1801.
28. Kliewer SA, Moore JT, Wade L, Staudinger JL, Watson MA, Jones SA, McKee DD, Oliver BB, Willson TM, Zetterstrom RH, Perlmann T, and Lehmann JM (1998) An orphan nuclear receptor activated by pregnanes defines a novel steroid signaling pathway. Cell 92:73-82.
29. Lehmann JM, McKee DD, Watson MA, Willson TM, Moore JT, and Kliewer SA (1998) The human orphan nuclear receptor PXR is activated by compounds that regulate CYP3A4 gene expression and cause drug interactions. J Clin Investig 102:1016-1023.
30. Lekstrom-Himes J and Xanthopoulos KG (1998) Biological role of the CCAAT/enhancer-binding protein family of transcription factors. J Biol Chem 273:28545-28548.
31. Matsumura K, Saito T, Takahashi Y, Ozeki T, Kiyotani K, Fujieda M, Yamazaki H, Kunitoh H, and Kamataki T (2004) Identification of a novel polymorphic enhancer of the human CYP3A4 gene. Mol Pharmacol 65:326-334.
32. Morel Y and Barouki R (1998) Down-regulation of cytochrome P450 1A1 gene promoter by oxidative stress. Critical contribution of nuclear factor 1. J Biol Chem 273:26969-26976.
33. Nelson D, Koymans L, Kamataki T, Stegeman J, Feyereisen R, Waxman D, Waterman M, Gotoh O, Coon M, Estabrook R, et al. (1996) P450 superfamily: update on new sequences, gene mapping, accession numbers and nomenclature. Pharmacogenetics 6:1-42.
34. Ourlin J, Jounaidi Y, Maurel P, and Vilarem M (1997) Role of the liver-enriched transcription factors C/EBP alpha and DBP in the expression of human CYP3A4 and CYP3A7. J Hepatol 26:54-62.
35. Pascussi J, Gerbal-Chaloin S, Drocourt L, Maurel P, and Vilarem MJ (2003) The expression of CYP2B6, CYP2C9 and CYP3A4 genes: a tangle of networks of nuclear and steroid receptors. Biochim Biophys Acta 1619:243-253.
36. Pascussi J, Gerbal-Chaloin S, Pichard-Garcia L, Daujat M, Fabre J, Maurel P, and Vilarem M (2000) Interleukin-6 negatively regulates the expression of pregnane X receptor and constitutively activated receptor in primary human hepatocytes. Biochem Biophys Res Commun 274:707-713.
37. Perez G, Tabares B, Jover R, Gomez-Lechon MJ, and Castell JV (2003) Semi-automatic quantitative RT-PCR to measure CYP induction by drugs in human hepatocytes. Toxicol In Vitro 17:643-649.
38. Peters LP and Teel RW (2003) Effects of high sucrose diet on body and liver weight and hepatic enzyme content and activity in the rat. In Vivo 17:61-65.
39. Poli V, Mancini F, and Cortese R (1990) IL-6DBP, a nuclear protein involved in interleukin-6 signal transduction, defines a new family of leucine zipper proteins related to C/EBP. Cell 63:643-653.
40. 1 phase of the hepatocyte cell cycle. J Biol Chem 270:18123-18132.
41. Rodriguez-Antona C, Bort R, Jover R, Tindberg N, Ingelman-Sundberg M, Gomez-Lechon MJ, and Castell JV (2003) Transcriptional regulation of human CYP3A4 basal expression by CCAAT enhancer-binding protein alpha and hepatocyte nuclear factor-3 gamma. Mol Pharmacol 63:1180-1189.
42. Rodriguez-Antona C, Jover R, Gomez-Lechon MJ, and Castell JV (2000) Quantitative RT-PCR measurement of human cytochrome P-450s: application to drug induction studies. Arch Biochem Biophys 376:109-116.
43. Sachdeva K, Yan B, and Chichester CO (2003) Lipopolysaccharide and cecal ligation/puncture differentially affect the subcellular distribution of the pregnane X receptor but consistently cause suppression of its target genes CYP3A. Shock 19:469-474.
44. Schuetz EG (2004) Lessons from the CYP3A4 promoter. Mol Pharmacol 65:279-281.
45. Tamasi V, Kiss A, Dobozy O, Falus A, Vereczkey L, and Monostory K (2001) The effect of dexamethasone on P450 activities in regenerating rat liver. Biochem Biophys Res Commun 286:239-242.
46. Tinel M, Robin M, Doostzadeh J, Maratrat M, Ballet F, Fardel N, el Kahwaji J, Beaune P, Daujat M, Labbe G, et al. (1995) The interleukin-2 receptor downregulates the expression of cytochrome P450 in cultured rat hepatocytes. Gastroenterology 109:1589-1599.
47. Tirona RG, Lee W, Leake BF, Lan LB, Cline CB, Lamba V, Parviz F, Duncan SA, Inoue Y, Gonzalez FJ, et al. (2003) The orphan nuclear receptor HNF4alpha determines PXR- and CAR-mediated xenobiotic induction of CYP3A4. Nat Med 9:220-224.
48. Weiner MP, Costa GL, Schoettlin W, Cline J, Mathur E, and Bauer JC (1994) Site-directed mutagenesis of double-stranded DNA by the polymerase chain reaction. Gene 151:119-123.
49. Welm A, Mackey S, Timchenko L, Darlington G, and Timchenk N (2000) Translational induction of liver-enriched transcriptional inhibitory protein during acute phase response leads to repression of CCAAT/enhancer binding protein alpha mRNA. J Biol Chem 275:27406-27413.
50. Wu J, Metz C, Xu X, Abe R, Gibson AW, Edberg JC, Cooke J, Xie F, Cooper GS, and Kimberly RP (2003) A novel polymorphic CAAT/enhancer-binding protein beta element in the FasL gene promoter alters Fas ligand expression: a candidate background gene in African American systemic lupus erythematosus patients. J Immunol 170:132-138.
51. Yamashita T, Hashimoto S, Kaneko S, Nagai S, Toyoda N, Suzuki T, Kobayashi K, and Matsushima K (2000) Comprehensive gene expression profile of a normal human liver. Biochem Biophys Res Commun 269:110-116.
52. Yamazoe Y, Murayama N, Shimada M, Yamauchi K, and Kato R (1989) Cytochrome P450 in livers of diabetic rats: regulation by growth hormone and insulin. Arch Biochem Biophys 268:567-575.