Semi-automatic quantitative RT-PCR to measure CYP induction by drugs in human hepatocytes

Toxicology in Vitro

Volumn 17, Issue 5-6, 2003, Pages 643-649

  Pérez, Gabriela ^a   Tabares, Beatriz ^b   Jover, Ramiro ^a,c   Gómez Lechón, Maria José ^a   Castell, José V ^a,c  

^a HOSPITAL UNIVERSITARIO LA FE   (Spain)

^b UNIVERSIDAD AUTÓNOMA DE MADRID   (Spain)

^c UNIVERSITY OF VALENCIA   (Spain)

Author keywords

CYP (human) RT PCR quantitation; CYP human induction; Cytochrome P 450; Drug; Human hepatocytes; Quantitative PCR; RT PCR

Indexed keywords

3 METHYLCHOLANTHRENE; COMPLEMENTARY DNA; CYTOCHROME P450; CYTOCHROME P450 1A1; CYTOCHROME P450 1A2; CYTOCHROME P450 2A6; CYTOCHROME P450 2B6; CYTOCHROME P450 2C9; CYTOCHROME P450 2D6; CYTOCHROME P450 2E1; CYTOCHROME P450 3A4; CYTOCHROME P450 3A5; ENZYME INDUCING AGENT; GREEN FLUORESCENT PROTEIN; OMEPRAZOLE; PHENOBARBITAL; RIFAMPICIN; RNA; RNA DIRECTED DNA POLYMERASE; TRABECTEDIN;

AUTOMATION; CONFERENCE PAPER; CONTROLLED STUDY; DRUG EFFECT; DRUG SCREENING; ENZYME INDUCTION; GENE AMPLIFICATION; HUMAN; HUMAN CELL; INCUBATION TIME; LIVER CELL; LIVER CELL CULTURE; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION;

EID: 0242266956     PISSN: 08872333     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0887-2333(03)00109-7     Document Type: Conference Paper

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