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Using ChIP assays to map the sites of DNA interaction of PolI, SL1 and UBF, these authors very convincingly demonstrated that in mouse, human and Xenopus cells, UBF is distributed rather equally throughout the whole rRNA gene repeat. As expected, PolI was found to be restricted to the 47S sequences and SL1 to the promoter. These data are fully consistent with several studies showing the lack of DNA sequence specificity in UBF-DNA complex formation and with the structural classification of its HMG1-box folds. The manuscript adds considerable weight to the idea that UBF defines an rRNA gene chromatin. Unfortunately, UBF is often erroneously referred to in the literature as a PolI promoter specific factor and often has unrealistic DNA sequence specific binding properties bestowed on it.
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O'Sullivan A.C., Sullivan G.J., McStay B. UBF binding in vivo is not restricted to regulatory sequences within the vertebrate ribosomal DNA repeat. Mol Cell Biol. 22:2002;657-668 Using ChIP assays to map the sites of DNA interaction of PolI, SL1 and UBF, these authors very convincingly demonstrated that in mouse, human and Xenopus cells, UBF is distributed rather equally throughout the whole rRNA gene repeat. As expected, PolI was found to be restricted to the 47S sequences and SL1 to the promoter. These data are fully consistent with several studies showing the lack of DNA sequence specificity in UBF-DNA complex formation and with the structural classification of its HMG1-box folds. The manuscript adds considerable weight to the idea that UBF defines an rRNA gene chromatin. Unfortunately, UBF is often erroneously referred to in the literature as a PolI promoter specific factor and often has unrealistic DNA sequence specific binding properties bestowed on it.
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This paper presented the revolutionary idea that a pool of inactive rRNA genes exists in mammalian cells. Subsequent work, reviewed in [29], showed this to be common to most, if not all, eukaryotes. Enhanced accessibility to psoralen has often erroneously been suggested to differentiate active and potentially active genes - that is, those with an 'open' chromatin configuration - on the one hand from epigenetically silenced ones on the other. In fact, enhanced psoralen accessibility occurs only within actively transcribed rRNA gene regions - even untranscribed spacer regions of active genes being indistinguishable from those of inactive genes ([29]; V Stefanovsky, T Moss, unpublished). Thus, psoralen accessibility cannot be used to differentiate an untranscribed but potentially active rRNA gene from an epigenetically silenced one.
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A key publication showing that, in yeast, transcription regulation and gene inactivation are separate phenomena. Psoralen crosslinking is used to show that little or no change in actively transcribed gene number occurs on PolI transcription down-regulation in the absence of histone deacetylase Rpd3p.
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Recruitment of the nucleolar remodeling complex NoRC establishes ribosomal DNA silencing in chromatin
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Strohner R., Nemeth A., Nightingale K.P., Grummt I., Becker P.B., Langst G. Recruitment of the nucleolar remodeling complex NoRC establishes ribosomal DNA silencing in chromatin. Mol Cell Biol. 24:2004;1791-1798.
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(2004)
Mol Cell Biol
, vol.24
, pp. 1791-1798
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Strohner, R.1
Nemeth, A.2
Nightingale, K.P.3
Grummt, I.4
Becker, P.B.5
Langst, G.6
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