Mimicking natural evolution in vitro: An N-acetylneuraminate lyase mutant with an increased dihydrodipicolinate synthase activity

Proceedings of the National Academy of Sciences of the United States of America

Volumn 100, Issue 10, 2003, Pages 5694-5699

  Joerger, Andreas C ^a   Mayer, Sebastian ^a   Fersht, Alan R ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

Aldolase; Engineering; Protein

Indexed keywords

BETA HYDROXYPYRUVATE; DIHYDRODIPICOLINATE SYNTHASE; ENZYME; N ACETYLNEURAMINATE LYASE; PYRUVIC ACID DERIVATIVE; UNCLASSIFIED DRUG;

ARTICLE; CIRCULAR DICHROISM; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME KINETICS; ENZYME SPECIFICITY; FEEDBACK SYSTEM; GENE MUTATION; GENETIC COMPLEMENTATION; IN VITRO STUDY; MOLECULAR CLONING; MOLECULAR EVOLUTION; PRIORITY JOURNAL; PROTEIN PURIFICATION; TURNOVER TIME;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; CLONING, MOLECULAR; CONSENSUS SEQUENCE; CRYSTALLOGRAPHY, X-RAY; ESCHERICHIA COLI; EVOLUTION, MOLECULAR; HYDRO-LYASES; KINETICS; MODELS, BIOLOGICAL; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; OXO-ACID-LYASES; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; THERMODYNAMICS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0038625074     PISSN: 00278424     EISSN: None     Source Type: Journal    
DOI: 10.1073/pnas.0531477100     Document Type: Article

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