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Volumn 125, Issue 34, 2003, Pages 10158-10159

Conversion of a PLP-dependent racemase into an aldolase by a single active site mutation

Author keywords

[No Author keywords available]

Indexed keywords

BENZALDEHYDE; FRUCTOSE BISPHOSPHATE ALDOLASE; GLYCINE; PHENYLSERINE; RACEMASE;

EID: 0041429626     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja036707d     Document Type: Article
Times cited : (96)

References (24)
  • 12
    • 0004136246 scopus 로고    scopus 로고
    • Cold Spring Harbor Laboratory Press: Cold Spring Harbor, NY
    • 2+-ion affinity chromatography, followed by anion-exchange on a monoQ column. Mutations were introduced using standard overlap-extension PCR methods (Sambrook, J.; Fritsch, E. F.; Maniatis, T. Molecular cloning: A laboratory manual, 3rd ed.; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, NY, 2001). The proteins were analyzed by electrospray ionization mass spectroscopy (ESI-MS) and shown to have the expected mass (wild-type Alr, found 44 648 ± 5 Da, expected 44 644 Da; Alr Y265A, found 44 561 ± 5 Da, expected 44 566 Da; Alr Y265A/H166N, found 44 539 ± 5 Da, expected 44 543 Da).
    • (2001) Molecular Cloning: A Laboratory Manual, 3rd Ed.
    • Sambrook, J.1    Fritsch, E.F.2    Maniatis, T.3
  • 15
    • 0041905379 scopus 로고    scopus 로고
    • note
    • a2 = 10.0 ± 0.3) (see Supporting Information). Reliable pH-rate data for the double mutant could not be obtained because of its low activity and instability at the pH extremes.
  • 23
    • 0041404272 scopus 로고    scopus 로고
    • note
    • Our preliminary results indicate that Alr Y265A also promotes the retroaldol reaction of D-α-methylthreonine which has not been reported for naturally occurring threonine aldolases.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.