Immune versus natural selection: Antibody aldolases with enzymic rates but broader scope

Science

Volumn 278, Issue 5346, 1997, Pages 2085-2092

  Barbas III, Carlos F ^a   Heine, Andreas ^a   Zhong, Guofu ^a   Hoffmann, Torsten ^a   Gramatikova, Svetlana ^a   Björnestedt, Robert ^a   List, Benjamin ^a   Anderson, James ^a   Stura, Enrico A ^a   Wilson, Ian A ^a   Lerner, Richard A ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYTIC ANTIBODY; FRUCTOSE BISPHOSPHATE ALDOLASE;

ARTICLE; DECARBOXYLATION; ENZYME REGULATION; ENZYME SPECIFICITY; ENZYME SUBSTRATE COMPLEX; GLYCOLYSIS; IMMUNIZATION; MOLECULAR RECOGNITION; PRIORITY JOURNAL;

ANIMALS; ANTIBODIES, CATALYTIC; BINDING SITES; CATALYSIS; CRYSTALLOGRAPHY, X-RAY; DECARBOXYLATION; EVOLUTION, MOLECULAR; FRUCTOSE-BISPHOSPHATE ALDOLASE; GLYCOLYSIS; HYDROGEN-ION CONCENTRATION; IMMUNIZATION; IMMUNOGLOBULIN FAB FRAGMENTS; KINETICS; LYSINE; MICE; MODELS, MOLECULAR; PROTEIN CONFORMATION; PYRIDOXAL; SELECTION (GENETICS); SUBSTRATE SPECIFICITY;

EID: 0031457319     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.278.5346.2085     Document Type: Article

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54. We thank W. Gilbert and A. Eschenmoser for helpful discussions; the Stanford Synchrotron Radiation Laboratory for data collection time; the staff of beamline 7-1 for their support; G. Fieser, R. Fuller, D. Kubitz, and R. Stefanko for technical assistance; M. Pique for production of figures; and the Alexander von Humboldt Foundation, Germany, for a Feodor Lynen Fellowship (T.H.). Supported by NIH CA27489 (R.A.L., I.A.W., C.F.B.). Coordinates and structure factors for the Fab' 33F12 have been deposited with Brookhaven Data Bank, with PDB ID code 1axt for the coordinate entry.