Biosynthesis of lipid-linked oligosaccharides in yeast: The ALG3 gene encodes the Dol-P-Man:Man5GlcNAc2-PP-Dol Mannosyltransferase

Biological Chemistry

Volumn 382, Issue 2, 2001, Pages 321-328

  Sharma, C B ^a   Knauer, R ^a   Lehle, L ^a  

^a UNIVERSITY OF REGENSBURG   (Germany)

Author keywords

ALG3; Dolichol; Endoplasmic reticulum; Lipid linked oligosaccharide; Mannosyltransferase; N glycosylation; Saccharomyces cerevisiae

Indexed keywords

ASPARAGINE LINKED OLIGOSACCHARIDE; CADMIUM; CALCIUM ION; DETERGENT; DOLICHOL PHOSPHATE MANNOSE; EPITOPE; FUNGAL ENZYME; GLUCOSE; GLYCOPROTEIN; GUANOSINE DIPHOSPHATE MANNOSE; LIPID; MAGNESIUM ION; MANGANESE; MANNOSE; MANNOSYLTRANSFERASE; METAL ION; N ACETYLGLUCOSAMINE; PYROPHOSPHATE; ZINC ION;

ARTICLE; BIOSYNTHESIS; CELL MEMBRANE; CHEMICAL BOND; CHEMICAL REACTION; CONTROLLED STUDY; CYTOPLASM; ENDOPLASMIC RETICULUM; ENZYME ACTIVITY; ENZYME ANALYSIS; FUNGAL GENETICS; GENE EXPRESSION; GENE MUTATION; GENETIC CODE; IMMUNOPRECIPITATION; IN VITRO STUDY; MOLECULAR BIOLOGY; MOLECULAR INTERACTION; NONHUMAN; PRIORITY JOURNAL; YEAST;

FUNGAL PROTEINS; LIPOPOLYSACCHARIDES; MANNOSYLTRANSFERASES; MEMBRANE PROTEINS; METALS; POLYISOPRENYL PHOSPHATE OLIGOSACCHARIDES; PRECIPITATION; SACCHAROMYCES CEREVISIAE PROTEINS; SOLUBILITY; YEASTS;

SACCHAROMYCES CEREVISIAE;

EID: 0035080711     PISSN: 14316730     EISSN: None     Source Type: Journal    
DOI: 10.1515/BC.2001.039     Document Type: Article

References (43)

1. Topography of glycosylation reactions in the endoplasmic reticulum
2. Cloning and characterization of the ALG3 gene of Saccharomyces cerevisiae
3. 2-P-P-dolichol in Chinese hamster ovary cells
4. The dolichol pathway of N-linked glycosylation
5. Stepwise assembly of the lipid-linked oligosaccharide in the endoplasmic reticulum of Saccharomyces cerevisiae: Identification of the ALG9 gene encoding a putative mannosyl transferase
6. Ordered assembly of the asymmetrically branched lipid-linked oligosaccharide in the endoplasmic reticulum is ensured by the substrate specificity of the individual glycosyl-transferases
7. The primary glycosylation defect in class E Thy-1-negative mutant mouse lymphoma cells is an inability to synthesize dolichol-P-mannose
8. 2-PP-dolichol in the Saccharomyces cerevisiae Δalg9 mutant reveals a regulatory role for the Alg3p α1,3-Man middle-arm addition in downstream oligosaccharide-lipid and glycoprotein glycan processing
9. Effect of energy depletion on the glycosylation of a viral glycoprotein
10. Biosynthesis of lipid-linked oligosaccharides. I. Preparation of lipid-linked oligosaccharide substrates
11. Applications of high efficiency lithium acetate transformation of intact yeast cells using single-stranded nucleic acids as carrier
12. 2-P-P-lipid in a Lec9 mutant of Chinese hamster ovary cells: Analysis of the steps in oligosaccharide-lipid assembly
13. Glycoprotein biosynthesis in yeast
14. Temperature-sensitive yeast mutants deficient in asparagine-linked glycosylation
15. Yeast mutants deficient in protein glycosylation
16. The N-oligosaccharyltransferase complex from yeast
17. The oligosaccharyltransferase complex from yeast
18. The oligosaccharyltransferase complex from Saccharomyces cerevisiae. Isolation of the OST6 gene, its synthetic interaction with OST3, and analysis of the native complex
19. Biochemistry and molecular biology of the N-oligosaccharyltransferase complex
20. 2-PP-dolichyl mannosyltransferase
21. Assembly of asparagine-linked oligosaccharides
22. Biosynthesis of the core region of yeast mannoproteins. Formation of a glucosylated dolichol-bound oligosaccharide precursor, its transfer to protein and subsequent modification
23. Protein glycosylation in yeast
24. Substrate specificity of N-acetylglucos-amine 1-phosphate transferase activity in Chinese hamster ovary cells
25. Three enzymes involved in oligosaccharide-lipid assembly in Chinese hamster ovary cells differ in lipid substrate preference
26. Specificity of solubilized yeast glycosyl transferases for polyprenyl derivatives
27. Identification of the mannosyl donors involved in the synthesis of lipid-linked oligosaccharides
28. Glucose starvation alters lipid-linked oligosaccharide biosynthesis in Chinese hamster ovary cells
29. A new yeast mutation in the glucosylation steps of the asparagine-linked glycosylation pathway. Formation of a novel asparagine-linked oligosaccharide containing two glucose residues
30. Two yeast mutations in glucosylation steps of the asparagine glycosylation pathway
31. SUC1 and SUC2: Two sucrose transporters from Arabidopsis thaliana; expression and characterization in baker's yeast and identification of the histidinetagged protein
32. Purification and characterization of UDP-N-acetyl-D-glucosamine:dolichol phosphate N-acetyl-D-glucosamine-1-phosphate transferase involved in the biosynthesis of asparagine-linked glycoproteins in the mammary gland
33. Solubilization and characterization of the initial enzymes of the dolichol pathway from yeast
34. 2-PP-dolichol mannosyltransferase
35. Biochemistry, molecular biology, and genetics of the oligosaccharyltransferase
36. New phenotype of mutations deficient in glucosylation of the lipid-linked oligosaccharide: Cloning of the ALG8 locus
37. Protein glycosylation in yeast
38. Modification of oligosaccharide-lipid synthesis and protein glycosylation in glucose-deprived cells
39. Glycoprotein biosynthesis in the alg3 Saccharomyces cerevisiae mutant. I. Role of glucose in the initial glycosylation of invertase in the endoplasmic reticulum
40. 2 processing intermediates on secreted invertase
41. 2 asparagine-linked glycans
42. STT3, a highly conserved protein required for yeast oligosaccharyl transferase activity in vivo