Engineering of an artificial glycosylation pathway blocked in core oligosaccharide assembly in the yeast Pichia pastoris: Production of complex humanized glycoproteins with terminal galactose

Glycobiology

Volumn 14, Issue 9, 2004, Pages 757-766

  Bobrowicz, Piotr ^a   Davidson, Robert C ^a   Li, Huijuan ^a   Potgieter, Thomas I ^a   Nett, Juergen H ^a   Hamilton, Stephen R ^a   Stadheim, Terrance A ^a   Miele, Robert G ^a   Bobrowicz, Beata ^a   Mitchell, Teresa ^a   Rausch, Sebastian ^a   Renfer, Eduard ^a   Wildt, Stefan ^a  

^a GlycoFi Inc   (Lebanon)

Author keywords

ALG3; Glycoprotein; N linked glycosylation; Oligosaccharide; Pichia

Indexed keywords

BETA 1,4 GALACTOSYLTRANSFERASE; DOLICHOL PHOSPHATE MANNOSE; GALACTOSE; GALACTOSYLTRANSFERASE; GLYCAN DERIVATIVE; GLYCOPROTEIN; HYBRID PROTEIN; MANNOSYLTRANSFERASE; OLIGOSACCHARIDE; UNCLASSIFIED DRUG; URIDINE DIPHOSPHATE GLUCOSE 4 EPIMERASE;

ARTICLE; COMBINATORIAL LIBRARY; COMPLEX FORMATION; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME STRUCTURE; ENZYME SUBSTRATE; EUKARYOTE; GENE DELETION; GENE EXPRESSION; GENE LIBRARY; GOLGI COMPLEX; IN VIVO STUDY; NONHUMAN; NUCLEOTIDE SEQUENCE; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN ENGINEERING; PROTEIN FUNCTION; PROTEIN GLYCOSYLATION; PROTEIN PROCESSING; PROTEIN SYNTHESIS; STRUCTURE ACTIVITY RELATION;

BASE SEQUENCE; DNA PRIMERS; GALACTOSE; GLYCOPROTEINS; GLYCOSYLATION; HUMANS; OLIGOSACCHARIDES; PICHIA; RECOMBINANT PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EUKARYOTA; MAMMALIA; PICHIA; PICHIA PASTORIS;

EID: 4444231014     PISSN: 09596658     EISSN: None     Source Type: Journal    
DOI: 10.1093/glycob/cwh104     Document Type: Article

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