Observation of a Short, Strong Hydrogen Bond in the Active Site of Hydroxynitrile Lyase from Hevea brasiliensis Explains a Large pKa Shift of the Catalytic Base Induced by the Reaction Intermediate

Journal of Biological Chemistry

Volumn 279, Issue 5, 2004, Pages 3699-3707

  Stranzl, Gudrun R ^a   Gruber, Karl ^a,c   Steinkellner, Georg ^a   Zangger, Klaus ^a   Schwab, Helmut ^b   Kratky, Christoph ^a  

^a UNIVERSITY OF GRAZ   (Austria)

^b GRAZ UNIVERSITY OF TECHNOLOGY   (Austria)

^c Angew Biokatalys Kompetenzzentrum   (Austria)

Author keywords

[No Author keywords available]

Indexed keywords

ALKALINITY; CATALYSIS; FREE ENERGY; HYDROGEN BONDS; NEGATIVE IONS; PH EFFECTS; SUBSTRATES;

CHEMICAL SHIFTS;

BIOCHEMISTRY;

CYANOHYDRIN; HISTIDINE; HYDROXYMANDELONITRILE LYASE; HYDROXYNITRILE LYASE; LYASE; NITRILE; PROTON; SERINE; THIOCYANATE; THIOCYANIC ACID DERIVATIVE; UNCLASSIFIED DRUG;

ARTICLE; BINDING COMPETITION; BINDING SITE; CATALYSIS; CHEMICAL MODEL; CHEMICAL STRUCTURE; CHEMISTRY; COMPETITIVE INHIBITION; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME SPECIFICITY; ENZYMOLOGY; GENE; HBHNL GENE; HEVEA; HEVEA BRASILIENSIS; HYDROGEN BOND; METABOLISM; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; OBSERVATION; PH; PKA; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTON TRANSPORT; THERMODYNAMICS; TIME;

ALDEHYDE-LYASES; BINDING SITES; BINDING, COMPETITIVE; CATALYSIS; HEVEA; HISTIDINE; HYDROGEN BONDING; HYDROGEN-ION CONCENTRATION; MAGNETIC RESONANCE SPECTROSCOPY; MODELS, CHEMICAL; MODELS, MOLECULAR; NITRILES; PROTONS; SUBSTRATE SPECIFICITY; THERMODYNAMICS; THIOCYANATES; TIME FACTORS;

HEVEA; HEVEA BRASILIENSIS;

EID: 0942298096     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M306814200     Document Type: Article

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