Elucidation of the mode of substrate binding to hydroxynitrile lyase from Hevea brasiliensis

Proteins: Structure, Function and Genetics

Volumn 44, Issue 1, 2001, Pages 26-31

  Gruber, Karl ^a  

^a UNIVERSITY OF GRAZ   (Austria)

Author keywords

Biocatalysis; C C couplings; Cyanohydrins; Docking; Enzyme mechanism; Molecular modeling

Indexed keywords

ACETONE; ACETONITRILE DERIVATIVE; ALDEHYDE DERIVATIVE; ALPHA HYDROXYNITRILE; ASPARTIC ACID; CYANOHYDRIN; HISTIDINE; HYDROXYNITRILE LYASE; LYSINE; SERINE; UNCLASSIFIED DRUG;

ARTICLE; ENZYME BINDING; ENZYME MECHANISM; ENZYME SPECIFICITY; ENZYME SUBSTRATE COMPLEX; HEVEA BRASILIENSIS; MOLECULAR MODEL; NONHUMAN; PH; PLANT; PRIORITY JOURNAL; REACTION ANALYSIS; STEREOCHEMISTRY; STRUCTURE ANALYSIS; THERMODYNAMICS;

ALDEHYDE-LYASES; BINDING SITES; COMPUTATIONAL BIOLOGY; EUPHORBIACEAE; LYSINE; MODELS, MOLECULAR; MOLECULAR CONFORMATION; NITRILES; SUBSTRATE SPECIFICITY; THERMODYNAMICS;

HEVEA BRASILIENSIS;

EID: 0035400446     PISSN: 08873585     EISSN: None     Source Type: Journal    
DOI: 10.1002/prot.1068     Document Type: Article

References (35)

1. Secondary plant products
2. Hydroxynitrile lyases: Functions and properties
3. Hydroxynitrile lyases of higher plants
4. Enzyme-catalyzed preparation and synthetic applications of optically-active cyanohydrins
5. Cyanohydrins in nature and the laboratory: Biology, preparations, and synthetic applications
6. Oxynitrilases - From cyanogenesis to asymmetric synthesis
7. Oxynitrilases for asymmetric C-C bond formation
8. Enzymatic C-C bond formation in asymmetric synthesis
9. Molecular cloning of the full-length cDNA of (S)-hydroxynitrile lyase from Hevea brasiliensis. Functional expression in Escherichia coli and Saccharomyces cerevisiae and identification of an active site residue
10. Purification and characterization of hydroxynitrile lyase from Hevea brasiliensis
11. Aliphatic (S)-cyanohydrins by enzyme catalysed synthesis
12. Synthesis of α,β-unsaturated (S)-cyanohydrins using the oxynitrilase from Hevea brasiliensis
13. Preparation of optically-active cyanohydrins using the (S)-hydroxynitrile lyase from Hevea brasiliensis
14. The chemoenzymatic synthesis of (S)-13-hydroxyoctadeca-(9z,11e)-dienoic acid using the hydroxynitrile lyase from Hevea brasiliensis
15. Enzyme-catalyzed formation of (S)-cyanohydrins derived from aldehydes and ketones in a biphasic solvent system
16. The α/β hydrolase fold
17. Mechanism of cyanogenesis: The crystal structure of hydroxynitrile lyase from Hevea brasiliensis
18. Hydroxynitrile lyase from Hevea brasiliensis: Molecular characterization and mechanism of enzyme catalysis
19. The serine proteinases
20. 3D structures of enzyme-substrate complexes of the hydroxynitrile lyase from Hevea brasiliensis
21. Mechanism of carbanion addition to carbonyl compounds. Equilibria and kinetics of substituted cyanohydrin cleavage and formation in aqueous solution. Substituted cyanohydrin proton dissociation constants
22. Identification of potential active-site residues in the hydroxynitrile lyase from Manihot esculenta by site-directed mutagenesis
23. Atomic resolution crystal structure of hydroxynitrile lyase from Hevea brasiliensis
24. Kinetic studies on the enzyme (S)-hydroxynitrile lyase from Hevea brasiliensis using initial rate methods and progress curve analysis
25. Automated docking of substrates to proteins by simulated annealing
26. Automated docking of flexible ligands: Applications of AutoDock
27. Application of the multimolecule and multiconformational RESP methodology to biopolymers: Charge derivation for DNA, RNA, and proteins
28. A 2nd generation force-field for the simulation of proteins, nucleic-acids, and organic molecules
29. Free-energy calculations: Applications to chemical and biochemical phenomena
30. Algorithms for macromolecular dynamics and constraint dynamics
31. MOLSCRIPT: A program to produce both detailed and schematic plots of protein structures
32. Raster3D: Photorealistic molecular graphics
33. Protein folding and association: Insights from the interracial and thermodynamic properties of hydrocarbons