NMR evidence for the participation of a low-barrier hydrogen bond in the mechanism of Δ5-3-ketosteroid isomerase

Proceedings of the National Academy of Sciences of the United States of America

Volumn 93, Issue 16, 1996, Pages 8220-8224

  Zhao, Qinjian ^a   Abeygunawardana, Chitrananda ^a   Talalay, Paul ^a   Mildvan, Albert S ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

catalysis; enolization; fractionation factor; ligand binding; proton transfer

Indexed keywords

DEUTERIUM; MUTANT PROTEIN; STEROID DELTA ISOMERASE;

AQUEOUS SOLUTION; ARTICLE; CATALYSIS; DIELECTRIC CONSTANT; ENZYME BINDING; ENZYME MECHANISM; HYDROGEN BOND; LIGAND BINDING; NUCLEAR OVERHAUSER EFFECT; PRIORITY JOURNAL; PROTON NUCLEAR MAGNETIC RESONANCE; PROTON TRANSPORT;

BACTERIAL PROTEINS; CATALYSIS; HYDROGEN BONDING; MAGNETIC RESONANCE SPECTROSCOPY; MUTAGENESIS, SITE-DIRECTED; PSEUDOMONAS; RECOMBINANT PROTEINS; STEROID ISOMERASES; STRUCTURE-ACTIVITY RELATIONSHIP;

PROTIUM;

EID: 0029737508     PISSN: 00278424     EISSN: None     Source Type: Journal    
DOI: 10.1073/pnas.93.16.8220     Document Type: Article

References (35)

1. Kuliopulos, A., Mildvan, A. S., Shortle, D. & Talalay, P. (1989) Biochemistry 28, 149-159.
2. Xue, L., Talalay, P. & Mildvan, A. S. (1990) Biochemistry 29, 7491-7500.
3. Kuliopulos, A., Talalay, P. & Mildvan, A. S. (1990) Biochemistry 29, 10271-10280.
4. Kuliopulos, A., Mullen, G. P., Xue, L. & Mildvan, A. S. (1991) Biochemistry 30, 3169-3178.
5. Xue, L., Talalay, P. & Mildvan, A. S. (1991) Biochemistry 30, 10858-10865.
6. Cleland, W. W. (1992) Biochemistry 31, 317-319.
7. Li, Y. K., Kuliopulos, A., Mildvan, A. S. & Talalay, P. (1993) Biochemistry 32, 1816-1824.
8. Gerlt, J. A. & Gassman, P. G. (1993) J. Am. Chem. Soc. 115, 11552-11568.
9. Cleland, W. W. & Kreevoy, M. M. (1994) Science 264, 1887-1890.
10. Zhao, Q., Mildvan, A. S. & Talalay, P. (1995) Biochemistry 34, 426-434.
11. Brooks, B. & Benisek, W. F. (1994) Biochemistry 33, 2682-2687.
12. Hawkinson, D. C., Pollack, R. M. & Ambulos, N. P., Jr. (1994) Biochemistry 33, 12172-12183.
13. Austin, J. C., Zhao, Q., Jordan, T., Talalay, P., Mildvan, A. S. & Spiro, T. G. (1995) Biochemistry 34, 4441-4447.
14. Zhao, Q., Abeygunawardana, C. & Mildvan, A. S. (1996) Biochemistry 35, 1525-1532.
15. Zhao, Q., Li, Y.-K., Mildvan, A. S. & Talalay, P. (1995) Biochemistry 34, 6562-6572.
16. Loh, S. N. & Markley, J. L. (1994) Biochemistry 33, 1029-1036.
17. Wang, S.-F., Kawahara, F. S. & Talalay, P. (1963) J. Biol. Chem. 238, 576-585.
18. Zeng, B., Bounds, P. L., Steiner, R. F. & Pollack, R. M. (1992) Biochemistry 31, 1521-1528.
19. Turner, D. L. (1983) J. Magn. Reson. 54, 146-148.
20. Frey, P. A., Whitt, S.A. & Tobin, J. B. (1994) Science 264, 1927-1930.
21. Liepinsch, E., Otting, G. & Wüthrich, K. (1992) J. Biomol. NMR 2, 447-465.
22. Kreevoy, M. M. & Liang, T. M. (1980) J. Am. Chem. Soc. 102, 3315-3322.
23. Hawkinson, D. C., Eames, T. C. M. & Pollack, R. M. (1991) Biochemistry 30, 6956-6964.
24. Shan, S. O., Loh, S. & Herschlag, D. (1996) Science 272, 97-101.
25. Hibbert, F. & Emsley, J. (1990) Adv. Phys. Org. Chem. 26, 255-379.
26. Warshel, A., Papazyan, A. & Kollman, P. A. (1995) Science 269, 102-103.
27. Frey, P. A. (1995) Science 269, 104-106.
28. Cleland, W. W. & Kreevoy, M. M. (1995) Science 269, 104.
29. Gunthrie, J. P. (1996) Chem. Biol. 3, 163-170.
30. Liang, T. C. & Abeles, R. H. (1987) Biochemistry 26, 7603-7608.
31. Robillard, G. & Shulman, R. G. (1974) J. Mol. Biol. 86, 519-540.
32. Markley, J. L. & Ibañez, I. B. (1978) Biochemistry 17, 4627-4640.
33. Kintanar, A., Metzler, C. M., Metzler, D. E. & Scott, R. D. (1991) J. Biol. Chem. 266, 17222-17229.
34. Tobin, J. B., Whitt, S. A., Cassidy, C. S. & Frey, P. A. (1995) Biochemistry 34, 6919-6924.
35. Smirnov, S. N., Golubeu, N. S., Denisov, G. S., Benedict, H., Schah-Mohammedi, P. & Limbach, H. H. (1996) J. Am. Chem. Soc. 118, 4094-4101.