Mechanistic aspects of cyanogenesis from active-site mutant Ser80Ala of hydroxynitrile lyase from Manihot esculenta in complex with acetone cyanohydrin

Protein Science

Volumn 10, Issue 5, 2001, Pages 1015-1022

  Lauble, Hanspeter ^a   Miehlich, Burkhard ^a   Förster, Siegfried ^a   Wajant, Harald ^a   Effenberger, Franz ^a  

^a UNIVERSITY OF STUTTGART   (Germany)

Author keywords

Acetone cyanohydrin complex; Cyanogenesis; Reaction mechanism; Site directed mutagenesis

Indexed keywords

ACETONE; ALANINE; CATION; CYANOHYDRIN; HYDROXYNITRILE LYASE; IMIDAZOLE DERIVATIVE; NITRILE; SERINE;

AMINO ACID SUBSTITUTION; ARTICLE; COMPLEX FORMATION; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME ANALYSIS; ENZYME BINDING; ENZYME KINETICS; ENZYME STRUCTURE; NONHUMAN; PLANT; PRIORITY JOURNAL; PROTON TRANSPORT; SITE DIRECTED MUTAGENESIS;

ALDEHYDE-LYASES; AMINO ACID SUBSTITUTION; BINDING SITES; CATALYSIS; CATALYTIC DOMAIN; CRYSTALLOGRAPHY, X-RAY; HAZARDOUS SUBSTANCES; KINETICS; MANIHOT; MODELS, MOLECULAR; MUTATION; NITRILES; PROTEIN BINDING; PROTEIN CONFORMATION; STRUCTURE-ACTIVITY RELATIONSHIP;

MANIHOT ESCULENTA;

EID: 0035056169     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.01301     Document Type: Article

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