Restriction enzyme-generated siRNA (REGS) vectors and libraries

Nature Genetics

Volumn 36, Issue 2, 2004, Pages 183-189

  Sen, George ^a   Wehrman, Tom S ^a   Myers, Jason W ^a   Blau, Helen M ^a  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; DOUBLE STRANDED DNA; OLIGONUCLEOTIDE; SMALL INTERFERING RNA;

ARTICLE; DIAGNOSTIC PROCEDURE; DIAGNOSTIC VALUE; DNA LIBRARY; ENZYME ASSAY; GENE SILENCING; MOLECULAR CLONING; MOLECULAR SIZE; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPE; PREDICTION; PRIORITY JOURNAL; RESTRICTION ENZYME GENERATED SMALL INTERFERING RNA TECHNOLOGY; RNA ANALYSIS; RNA VECTOR; TRANSGENE;

ANIMALS; DNA RESTRICTION ENZYMES; GENE SILENCING; GENETIC VECTORS; GENOMIC LIBRARY; MICE; MYOBLASTS; RNA, SMALL INTERFERING;

ANIMALIA; MAMMALIA;

EID: 0842310832     PISSN: 10614036     EISSN: None     Source Type: Journal    
DOI: 10.1038/ng1288     Document Type: Article

References (28)

1. Lee, S.S. et al. A systematic RNAi screen identifies a critical role for mitochondria in C. elegans longevity. Nat. Genet. 33, 40-48 (2003).
2. Carthew, R.W. Gene silencing by double-stranded RNA. Curr. Opin. Cell Biol. 13, 244-248 (2001).
3. Shi, Y. Mammalian RNAi for the masses. Trends Genet. 19, 9-12 (2003).
4. Fire, A. et al. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391, 806-811 (1998).
5. Kamath, R.S. et al. Systematic functional analysis of the Caenorhabditis elegans genome using RNAi. Nature 421, 231-237 (2003).
6. Dykxhoorn, D.M., Novina, C.D. & Sharp, P.A. Killing the messenger: short RNAs that silence gene expression. Nat. Rev. Mol. Cell Biol. 4, 457-467 (2003).
7. Elbashir, S.M. et al. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 411, 494-498 (2001).
8. Zamore, P.D., Tuschl, T., Sharp, P.A. & Bartel, D.P. RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals. Cell 101, 25-33 (2000).
9. Sui, G. et al. A DNA vector-based RNAi technology to suppress gene expression in mammalian cells. Proc. Natl. Acad. Sci. USA 99, 5515-5520 (2002).
10. Yu, J.Y., DeRuiter, S.L. & Turner, D.L. RNA interference by expression of short-interfering RNAs and hairpin RNAs in mammalian cells. Proc. Natl. Acad. Sci. USA 99, 6047-6052 (2002).
11. Paul, C.P., Good, P.D., Winer, I. & Engelke, D.R. Effective expression of small interfering RNA in human cells. Nat. Biotechnol. 20, 505-508 (2002).
12. Brummelkamp, T.R., Bernards, R. & Agami, R. A system for stable expression of short interfering RNAs in mammalian cells. Science 296, 550-553 (2002).
13. Lee, N.S. et al. Expression of small interfering RNAs targeted against HIV-1 rev transcripts in human cells. Nat. Biotechnol. 20, 500-505 (2002).
14. Miyagishi, M. & Taira, K. U6 promoter-driven siRNAs with four uridine 3′ overhangs efficiently suppress targeted gene expression in mammalian cells. Nat. Biotechnol. 20, 497-500 (2002).
15. Paddison, P.J., Caudy, A.A., Bernstein, E., Hannon, G.J. & Conklin, D.S. Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells. Genes Dev. 16, 948-958 (2002).
16. Rubinson, D.A. et al. A lentivirus-based system to functionally silence genes in primary mammalian cells, stem cells and transgenic mice by RNA interference. Nat. Genet. 33, 401-406 (2003).
17. Brummelkamp, T.R., Bernards, R. & Agami, R. Stable suppression of tumorigenicity by virus-mediated RNA interference. Cancer Cell 2, 243-247 (2002).
18. Matsukura, S., Jones, P.A. & Takai, D. Establishment of conditional vectors for hairpin siRNA knockdowns. Nucleic Acids Res. 31, e77 (2003).
19. Jackson, A.L. et al. Expression profiling reveals off-target gene regulation by RNAi. Nat. Biotechnol. 21, 635-637 (2003).
20. Detter, J.C. et al. Isothermal strand-displacement amplification applications for high-throughput genomics. Genomics 80, 691-698 (2002).
21. Boyd, A.C., Charles, I.G., Keyte, J.W. & Brammar, W.J. Isolation and computer-aided characterization of Mmel, a type II restriction endonuclease from Methylophilus methylotrophus. Nucleic Acids Res. 14, 5255-5274 (1986).
22. Czauderna, F. et al. Functional studies of the PI(3)-kinase signalling pathway employing synthetic and expressed siRNA. Nucleic Acids Res. 31, 670-682 (2003).
23. Niwa, H., Miyazaki, J. & Smith, A.G. Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells. Nat. Genet. 24, 372-376 (2000).
24. Okuda, A. et al. UTF1, a novel transcriptional coactivator expressed in pluripotent embryonic stem cells and extra-embryonic cells. EMBO J. 17, 2019-2032 (1998).
25. Tanaka, T.S. et al. Gene expression profiling of embryo-derived stem cells reveals candidate genes associated with pluripotency and lineage specificity. Genome Res. 12, 1921-1928 (2002).
26. Cornelison, D.D., Olwin, B.B., Rudnicki, M.A. & Wold, B.J. MyoD(-/-) satellite cells in single-fiber culture are differentiation defective and MRF4 deficient. Dev. Biol. 224, 122-137 (2000).
27. Hannon, G.J. RNA interference. Nature 418, 244-251 (2002).
28. Rando, T.A. & Blau, H.M. Primary mouse myoblast purification, characterization, and transplantation for cell-mediated gene therapy. J. Cell. Biol. 125, 1275-1287 (1994).