Crystal structure of an in vitro affinity- and specificity-matured anti-testosterone Fab in complex with testosterone: Improved affinity results from small structural changes within the variable domains

Journal of Biological Chemistry

Volumn 277, Issue 46, 2002, Pages 44021-44027

  Valjakka, Jarkko ^a   Hemminki, Ari ^b   Niemi, Seija ^c   Söderlund, Hans ^d   Takkinen, Kristiina ^d   Rouvinen, Juha ^a  

^a UNIVERSITY OF EASTERN FINLAND   (Finland)

^b Finnish Red Cross   (Finland)

^c Orion Diagnostica Oy   (Finland)

^d VTT TECHNICAL RESEARCH CENTRE OF FINLAND   (Finland)

Author keywords

[No Author keywords available]

Indexed keywords

CONFORMATIONS; CRYSTAL STRUCTURE; HORMONES; MONOCLONAL ANTIBODIES; MUTAGENESIS; PROTEINS;

BINDING PROPERTIES;

BIOCHEMISTRY;

ALANINE; GLUTAMINE; IMMUNOGLOBULIN F(AB) FRAGMENT; TESTOSTERONE;

ARTICLE; BINDING AFFINITY; BINDING SITE; COMPLEMENTARITY DETERMINING REGION; CRYSTAL STRUCTURE; HORMONE BINDING; HORMONE PROTEIN COMPLEX; HUMAN; MUTAGENESIS; NONHUMAN; PHAGE DISPLAY; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN DOMAIN;

AMINO ACID SEQUENCE; ANTIBODIES, MONOCLONAL; BINDING SITES; CRYSTALLOGRAPHY, X-RAY; FUNGAL PROTEINS; GENE LIBRARY; HUMANS; KINETICS; MEMBRANE TRANSPORT PROTEINS; MODELS, CHEMICAL; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS; MUTATION; PEPTIDE LIBRARY; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; SEQUENCE HOMOLOGY, AMINO ACID; TESTOSTERONE; TIME FACTORS;

EID: 0345829975     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M208392200     Document Type: Article

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