Improving the affinity and the fine specificity of an anti-cortisol antibody by parsimonious mutagenesis and phage display

Journal of Immunology

Volumn 161, Issue 10, 1998, Pages 5421-5429

  Chames, Patrick ^a,b   Coulon, Phane ^a   Baty, Daniel ^a  

^a CNRS   (France)

^b MAASTRICHT UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

HORMONE ANTIBODY; HYDROCORTISONE; MONOCLONAL ANTIBODY;

ANTIBODY AFFINITY; ANTIBODY PRODUCTION; ANTIGEN RECOGNITION; ARTICLE; IMMUNOASSAY; NONHUMAN; PRIORITY JOURNAL; STEROID ANALYSIS;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMALS; ANTIBODIES, MONOCLONAL; ANTIBODY AFFINITY; ANTIBODY SPECIFICITY; BACTERIOPHAGE M13; BINDING, COMPETITIVE; CLONING, MOLECULAR; HYDROCORTISONE; IMMUNOGLOBULIN VARIABLE REGION; MICE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PEPTIDE LIBRARY;

EID: 0032533803     PISSN: 00221767     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (47)

1. Milgrom, E. 1990. Steroid hormones. In Hormones: From Molecules to Disease, E. E. Baulieu and P. A. Kelly PA, eds. Hermann, Paris, p. 385.
2. Yoon, D. Y., M. J. Choi, I. S. Choe, T. W. Chung, and S. M. Byun. 1993. Influence of the conjugation site on the specificity of monoclonal antibodies to progesterone and on the performance of direct enzyme immunoassay. Biochem. Mol. Biol. Int. 31:553.
3. Gani, M., J. Coley, J. Piron, A. S. Humphreys, J. Arevalo, I. A. Wilson, and M. J. Taussig. 1994. Monoclonal antibodies against progesterone: effect of steroid-carrier coupling position on antibody specificity. J. Steroid Biochem. Mol. Biol. 48:277.
4. Arevalo, J. H., C. A. Hassig, E. A. Stura, M. J. Sims, M. J. Taussing, and I. A. Wilson. 1994. Structural analysis specificity: detailed comparison of five Fab-steroid complexes. J. Mol. Biol. 241:663.
5. Barkley, M. S., B. L. Lasley, M. A. Thompson, and C. H. Shackleton. 1995. Equol: a contributor to enigmatic immunoassay measurements of estrogen. Steroids 46:587.
6. Agasan, A. L., B. J. Stewart, and T. G. Watson. 1994. Development of a radioimmunoassay method for ethynylestradiol in plasma using a monoclonal antibody. J. Immunol. Methods 177:251.
7. Wudt, S. A., U. A. Wachter, J. Homoki, and W. M. Teller. 1995. 17α-Hydroxyprogesterone, 4-androstenedione, and testosterone profiled by routine stable isotope dilution/gas chromatography-mass spectrometry in plasma of children. Pediatr. Res. 38:76.
8. Roberts, S., J. C. Cheetham, and A. R. Rees. 1987. Generation of an antibody with enhanced affinity and specificity for its antigen by protein engineering. Nature 328:731.
9. Sharon, J. 1990. Structural correlates of high antibody affinity: three engineered amino acid substitutions can increase the affinity of anti-p-azophenylarsonate antibody 200-fold. Proc. Natl. Acad. Sci. USA 87:4814.
10. Kussie, P. H., B. Parhami-Seren, L. J. Wysocki, and M. N. Margolies. 1994. A single engineered amino acid substitution changes antibody fine specificity. J. Immunol. 152:146.
11. Marks, J. D., H. R. Hoogenboom, A. D. Griffiths, and G. Winter. 1992. Molecular evolution of proteins on filamentous phage: mimicking the strategy of the immune system. J. Biol. Chem. 267:16007.
12. Glaser, S. M., D. E. Yelton, and W. D. Huse. 1992. Antibody engineering by codon-based mutagenesis in a filamentous phage vector system. J. Immunol. 149: 3903.
13. Deng, S. J., C. R. MacKenzie, J. Sodowska, J. Michniewicz. N. M. Young, D. R. Bundle, and S. A. Narang. 1994. Selection of antibody single-chain variable fragments with improved carbohydrate binding by phage display. J. Biol. Chem. 269:9533.
14. Barbas, C. F., D. Hu, N. Dunlop, L. Sawyer, D. Cababa, R. M. Hendry, P. L. Nara, and D. R. Burton. 1994. In vitro evolution of a neutralizing human antibody to human immunodeficiency virus type 1 to enhance affinity and broaden strain cross-reactivity. Proc. Natl. Acad. Sci. USA 91:3809.
15. H region. J. Immunol. 155: 5647.
16. Schier, R., A. McCall, G. P. Adams, K. W. Marshall, H. Merritt, M. Yim, R. S. Crawford, L. M. Weiner, C. Marks, and J. D. Marks. 1996. Isolation of picomolar affinity anti-c-erbB-2 single-chain Fv by molecular evolution of the complementarity determining regions in the center of the antibody binding site. J. Mol. Biol. 263:551.
17. Le Calvez, H., J. Fieschi, J. M. Green, N. Marchesi, J. Chauveau, and D. Baty. 1995. Paratope characterization by structural modelling of two anti-cortisol single-chain variable fragments produced in E. coli. Mol. Immunol. 32:185.
18. Whitlow, M., B. A. Bell, S. L. Feng, D. Filpula, K. D. Hardman, S. L. Hubert, M. L. Rollence, J. F. Wood, M. E. Schott, D. E. Milenic, T. Yokota, and J. Schlom. 1993. An improved linker for single-chain Fv with reduced aggregation and enhanced proteolytic stability. Protein Eng. 6:989.
19. Norton, R. M., H. D. Hunt, S. N. Ho, J. K. Pullen, and L. R. Pease. 1989. Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension. Gene 77:61.
20. H gene segments rearranged in vitro. J. Mol. Biol. 227:381.
21. Pluckthun, A., and A. Skerra. 1989. Expression of functional antibody Fv and Fab fragments in Escherichia coli. Methods Enzymol. 178:497.
22. Friguet, B., A. F. Chaffotte, L. Djavadi-Ohaniance, and M. E. Goldberg. 1985. Measurements of the true affinity constant in solution of antigen-antibody complexes by enzyme-linked immunosorbent assay. J. Immunol. Methods 77:305.
23. Kabat, E. A., T. T. Wu, H. M. Perry, K. S. Gottesman, and C. Foeller. 1991. Sequences of Proteins of Immunological Interest, 5th Ed. U.S. Department of Health and Human Services, Bethesda.
24. Arevalo, J. H., M. J. Taussig, and I. A. Wilson. 1993. Molecular basis of cross-reactivity and the limits of antibody-antigen complementarity. Nature 365:859.
25. MacCallum, R. M., A. C. Martin, and J. M. Thornton. 1996. Antibody-antigen interactions: contact analysis and binding site topography. J. Mol. Biol. 262:732.
26. Parhami-Seren, B., P. H. Kussie, R. K. Strong, and M. N. Margolies. 1993. Conservation of binding site geometry among p-azophenylarsonate-specific antibodies. J. Immunol. 150:1829.
27. Balint, R. F., and J. W. Larrick. 1993. Antibody engineering by parsimonious mulagenesis. Gene 137:109.
28. Schier, R., R. F. Balint, A. McCall, G. Apell, J. W. Larrick, and J. D. Marks. 1996. Identification of functional and structural amino-acid residues by parsimonious mutagenesis. Gene 169:147.
29. Eigenbrot, C. M. Randal, L. Presta, P. Carter, and A. A. Kossiakoff. 1993. X-ray structures of the antigen-binding domains from three variants of humanized antip185HER2 antibody 4D5 and comparison with molecular modeling. J. Mol. Biol. 229:969.
30. Barry, M. M., C. D. Mol, W. F. Andersen, and J. S. Lee. 1994. Sequencing and modeling of anti-DNA immunoglobulin Fv domains: comparison with crystal structures. J. Biol. Chem. 269:3623.
31. Chothia, C., A. M. Lesk, A. Tramontano, M. Levitt, S. J. Smith-Gill, G. Air, S. Sheriff, E. A. Padlan, D. Davies, W. R. Tulip, et al. 1989. Conformations of immunoglobulin hypervariable regions. Nature 342:877.
32. Schiweck, W., and A. Skerra. 1997. The rational construction of an antibody against cystatin: lessons from the crystal structure of an artificial Fab fragment. J. Mol. Biol. 268:934.
33. Vaughan, T. J., A. J. Williams, K. Pritchard, J. K. Osbourn, A. R. Pope, J. C. Earnshaw, J. MacCafferty, R. A. Hodits, J. Wilton, and K. S. Johnson. 1996 Human antibodies with sub-nanomolar affinities isolated from a large non-immunized phage display library. Nat. Biotechnol. 14:309.
34. Lavoie, T. B., W. N. Drohan, and S. J. Smith-Gill. 1992. Experimental analysis by site-directed mutagenesis of somatic mutation effects on affinity and fine specificity in antibodies specific for lysozyme. J. Immunol 148:503.
35. Xiang, J., Y. Sha, L. Prasad, and L. T. Delbaere. 1996. Complementarity determining region residues aspartic acid at H55, serine at H95 and tyrosines at H97 and L96 play important roles in the B72.3 antibody-TAG72 antigen interaction. Protein Eng. 9:539.
36. Wedemayer, G. J., P. A. Patten, L. H. Wang, P. G. Schultz, and R. C. Stevens. 1997. Structural insights into the evolution of an antibody combining site. Science 276:1665.
37. Allen, D., T. Simon, F. Sablitzky, K. Rajewsky, and A. Cumano. 1988. Antibody engineering for the analysis of affinity maturation of an anti-hapten response. EMBO J. 7:1995.
38. Glockshuber, R., J. Stadlmuller, and A. Pluckthun. 1991. Mapping and modification of an antibody hapten binding site: a site-directed mutagenesis study of McPC603. Biochemistry 30:3049.
39. Mian, I. S., A. R. Bradwell, and A. J. Olson. 1991. Structure, function and properties of antibody binding sites. J. Mol. Biol. 217:133.
40. Denzin, L. K., G. A. Gulliver, and E. W. Voss, Jr 1993 Mutational analysis of active site contact residues in anti-fluorescein monoclonal antibody 4-4-20. Mol. Immunol. 30:1331.
41. Ruff-Jamison, S., and J. R. Glenney. 1993. Molecular modeling and site-directed mutagenesis of an anti-phosphotyrosine antibody predicts the combining site and allows the detection of higher affinity interactions. Protein Eng. 6:661.
42. Schildbach, J. F., R. I. Near, R. E. Bruccoleri, E. Haber, P. D. Jeffrey, S. C. Ng, J. Novotny, S. Sheriff, and M. N. Margolies. 1993. Heavy chain position 50 is a determinant of affinity and specificity for the anti-digoxin antibody 26-10. J. Biol. Chem. 268:21739.
43. Sompuram, S. R., and J. Sharon. 1993. Verification of a model of a F(ab) complex with phenylarsonate by oligonucleotide-directed mutagenesis. J. Immunol. 150:1822.
44. Stewart, J. D., V. A. Roberts, N. R. Thomas, E. D. Getzoff, and S. J. Benkovic. 1994. Site-directed mutagenesis of a catalytic antibody: an arginine and a histidine residue play key roles. Biochemistry 33:1994.
45. Short, M. K., P. D. Jeffrey, R. F. Kwong, and M. N. Margolies. 1995. Contribution of antibody heavy chain CDR1 to digoxin binding analyzed by random mutagenesis of phage-displayed Fab 26-10. J. Biol. Chem. 270:28541.
46. Sotriffer, C. A., K. R. Liedl, R. H. Winger, A. M. Gamper, R. T. Kroemer, D. S. Linthicum, B. M. Rode, and J. M. Varga. 1996. Heteroligation of a mouse monoclonal IgE antibody (La2) with small molecules, analysed by computer-aided automated docking. Mol. Immunol. 33:129.
47. Burks, E. A., G. Chen, G. Georgiou, and B. L. Iverson. 1997. In vitro scanning saturation mutagenesis of an antibody binding pocket. Proc. Natl. Acad. Sci. USA 94:412.