Fine tuning of an anti-testosterone antibody binding site by stepwise optimisation of the CDRs

Immunotechnology

Volumn 4, Issue 1, 1998, Pages 59-69

  Hemminki, Ari ^a   Niemi, Seija ^b   Hautoniemi, Lasse ^b   Söderlund, Hans ^a   Takkinen, Kristiina ^a  

^a VTT TECHNICAL RESEARCH CENTRE OF FINLAND   (Finland)

^b Orion Diagnostica Oy   (Finland)

Author keywords

CDR mutagenesis; Competitive and affinity panning; Recombinant Fab; Specificity and affinity improvement; Testosterone

Indexed keywords

HORMONE ANTIBODY; IMMUNOGLOBULIN F(AB) FRAGMENT; IMMUNOGLOBULIN HEAVY CHAIN; IMMUNOGLOBULIN LIGHT CHAIN; PRASTERONE; TESTOSTERONE;

ANTIBODY COMBINING SITE; ANTIBODY STRUCTURE; ARTICLE; COMPLEMENTARITY DETERMINING REGION; MOLECULAR CLONING; MUTAGENESIS; MUTANT; PHAGE DISPLAY; PRIORITY JOURNAL;

ANTIBODY AFFINITY; BINDING SITES, ANTIBODY; CLONING, MOLECULAR; CROSS REACTIONS; DEHYDROEPIANDROSTERONE; EVALUATION STUDIES; GENE LIBRARY; HUMANS; IMMUNOASSAY; IMMUNOGLOBULIN FAB FRAGMENTS; IMMUNOGLOBULIN VARIABLE REGION; KINETICS; MUTAGENESIS; MUTATION; TESTOSTERONE;

EID: 0031810912     PISSN: 13802933     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1380-2933(98)00002-5     Document Type: Article

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