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1
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0024833055
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Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda
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Burton, D.R.6
Benkovic, S.J.7
Lerner, R.A.8
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2
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0021818675
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Barbas C.F. III1
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0028672525
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Human antibodies from combinatorial libraries
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Burton DR, Barbas CF III: Human antibodies from combinatorial libraries. Adv Immunol 1994, 57:191-280.
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Burton, D.R.1
Barbas C.F. III2
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5
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0031081318
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Designing and optimizing library selection strategies for generating high-affinity antibodies
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Hoogenboom HR: Designing and optimizing library selection strategies for generating high-affinity antibodies. Trends Biotechnol 1997, 15:62-70.
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Trends Biotechnol
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Hoogenboom, H.R.1
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6
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0029564921
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CDR walking mutagenesis for the affinity maturation of a potent human anti-HIV-1 antibody into the picomolar range
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Yang WP, Green K, Pinz-Sweeney S, Briones AT, Burton DR, Barbas CF III: CDR walking mutagenesis for the affinity maturation of a potent human anti-HIV-1 antibody into the picomolar range. J Mol Biol 1995, 254:392-403.
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J Mol Biol
, vol.254
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Yang, W.P.1
Green, K.2
Pinz-Sweeney, S.3
Briones, A.T.4
Burton, D.R.5
Barbas C.F. III6
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7
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0030200088
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Selection and evolution of high-affinity human anti-viral antibodies
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Barbas CF III, Burton DR: Selection and evolution of high-affinity human anti-viral antibodies. Trends Biotechnol 1996, 14:230-234.
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Trends Biotechnol
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Barbas C.F. III1
Burton, D.R.2
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8
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0029957873
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Human antibody responses to HIV-1 gp41 cloned in phage display libraries suggest three major epitopes are recognized and give evidence for conserved antibody motifs in antigen binding
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Binley JM, Ditzel HJ, Barbas CF III, Sullivan N, Sodroski J, Parren PWHI, Burton DR: Human antibody responses to HIV-1 gp41 cloned in phage display libraries suggest three major epitopes are recognized and give evidence for conserved antibody motifs in antigen binding. AIDS Res Hum Retroviruses 1996, 12:911-924.
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AIDS Res Hum Retroviruses
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Binley, J.M.1
Ditzel, H.J.2
Barbas C.F. III3
Sullivan, N.4
Sodroski, J.5
Parren, P.W.H.I.6
Burton, D.R.7
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9
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0029665877
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A melanoma-specific VH antibody cloned from a fusion phage library of a vaccinated melanoma patient
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Cai X, Garen A: A melanoma-specific VH antibody cloned from a fusion phage library of a vaccinated melanoma patient Proc Natl Acad Sci USA 1996, 93:6280-6285.
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Proc Natl Acad Sci USA
, vol.93
, pp. 6280-6285
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Cai, X.1
Garen, A.2
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10
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0029843718
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In vitro immunization of naive human B cells yields high affinity immunoglobulin G antibodies as illustrated by phage display
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Duenas M, Chin LT, Malmborg AC, Casalvilla R, Ohlin M, Borrebaeck CAK: In vitro immunization of naive human B cells yields high affinity immunoglobulin G antibodies as illustrated by phage display. Immunology 1996, 89:1-7.
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Immunology
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, pp. 1-7
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Duenas, M.1
Chin, L.T.2
Malmborg, A.C.3
Casalvilla, R.4
Ohlin, M.5
Borrebaeck, C.A.K.6
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11
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0029870943
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Phage display cloning and characterization of an immunogenetic marker (perinuclear anti-neutrophil cytoplasmic antibody) in ulcerative colitis
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Eggena M, Targan SR, Iwanczyk L, Vidrich A, Gordon LK, Braun J: Phage display cloning and characterization of an immunogenetic marker (perinuclear anti-neutrophil cytoplasmic antibody) in ulcerative colitis. J Immunol 1996, 156:4005-4011.
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J Immunol
, vol.156
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Eggena, M.1
Targan, S.R.2
Iwanczyk, L.3
Vidrich, A.4
Gordon, L.K.5
Braun, J.6
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12
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0028886129
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A human monoclonal antibody specific for the leucine-33 (P1A1, HPA-1a) form of platelet glycoprotein IIIa from a V gene phage display library
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Griffin HM, Ouwehand WH: A human monoclonal antibody specific for the leucine-33 (P1A1, HPA-1a) form of platelet glycoprotein IIIa from a V gene phage display library. Blood 1995, 86:4430-4436.
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(1995)
Blood
, vol.86
, pp. 4430-4436
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Griffin, H.M.1
Ouwehand, W.H.2
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13
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0029949635
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Cloning and analysis of IgGκ and IgGλ anti-thyroglobulin autoantibodies from a patient with Hashimoto's thyroiditis: Evidence for in vivo antigen-driven repertoire selection
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McIntosh RS, Asghar MS, Watson PF, Kemp EH, Weetman AP: Cloning and analysis of IgGκ and IgGλ anti-thyroglobulin autoantibodies from a patient with Hashimoto's thyroiditis: evidence for in vivo antigen-driven repertoire selection. J Immunol 1996, 157:927-935.
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(1996)
J Immunol
, vol.157
, pp. 927-935
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McIntosh, R.S.1
Asghar, M.S.2
Watson, P.F.3
Kemp, E.H.4
Weetman, A.P.5
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14
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0030152049
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Characteristics of human antibody repertoires following active immune responses in vivo
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Ohlin M, Borrebaeck CAK: Characteristics of human antibody repertoires following active immune responses in vivo. Mol Immunol 1996, 33:583-592.
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(1996)
Mol Immunol
, vol.33
, pp. 583-592
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Ohlin, M.1
Borrebaeck, C.A.K.2
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15
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0029894389
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Recombinant human Fab antibody fragments to HIV-1 Rev and Tat regulatory proteins: Direct selection from a combinatorial phage display library
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Pilkington GR, Duan L, Zhu M, Keil W, Pomerantz RJ: Recombinant human Fab antibody fragments to HIV-1 Rev and Tat regulatory proteins: direct selection from a combinatorial phage display library. Mol Immunol 1996, 33:439-450.
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(1996)
Mol Immunol
, vol.33
, pp. 439-450
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Pilkington, G.R.1
Duan, L.2
Zhu, M.3
Keil, W.4
Pomerantz, R.J.5
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16
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0030453429
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Repertoire cloning of lupus anti-DNA autoantibodies
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Roben P, Barbas SM, Sandoval L, Lecerf JM, Stollar BD, Solomon A, Silverman GJ: Repertoire cloning of lupus anti-DNA autoantibodies. J Clin Invest 1996, 98:2827-2837.
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(1996)
J Clin Invest
, vol.98
, pp. 2827-2837
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Roben, P.1
Barbas, S.M.2
Sandoval, L.3
Lecerf, J.M.4
Stollar, B.D.5
Solomon, A.6
Silverman, G.J.7
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17
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0029826106
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Human antibodies from phage libraries: Neutralizing activity against human immunodeficiency virus type 1 equally improved after expression as Fab and IgG in mammalian cells
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Samuelsson A, Yari F, Hinkula J, Ersoy O, Norrby E, Persson MAA: Human antibodies from phage libraries: neutralizing activity against human immunodeficiency virus type 1 equally improved after expression as Fab and IgG in mammalian cells. Eur J Immunol 1996, 26:3029-3034. This report suggests that Fab expressed in E. coli is not identical, in terms of binding and virus neutralization, to antibody expressed in eukaryotic cells. Antibody derived from expression in a eukaryotic host may be superior in terms of the fraction of properly folded protein that is produced as compared with that expressed in prokaryotes. This observation may be antibody dependent.
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(1996)
Eur J Immunol
, vol.26
, pp. 3029-3034
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Samuelsson, A.1
Yari, F.2
Hinkula, J.3
Ersoy, O.4
Norrby, E.5
Persson, M.6
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18
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0029555112
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Isolation of human anti-red blood cell antibodies by repertoire cloning
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Siegel DL: Isolation of human anti-red blood cell antibodies by repertoire cloning. Ann NY Acad Sci 1995, 764:547-558.
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(1995)
Ann NY Acad Sci
, vol.764
, pp. 547-558
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Siegel, D.L.1
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19
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0030588617
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An endogenous sialoprotein and a bacterial B cell superantigen compete in their VH family-specific binding interactions with human Igs
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Silverman GJ, Pires R, Bouvet JP: An endogenous sialoprotein and a bacterial B cell superantigen compete in their VH family-specific binding interactions with human Igs. J Immunol 1996, 157:4496-4502. An interesting report on the investigation of superantigen interactions as probed with libraries.
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(1996)
J Immunol
, vol.157
, pp. 4496-4502
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Silverman, G.J.1
Pires, R.2
Bouvet, J.P.3
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20
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0029919595
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Construction of a combinatorial IgE library from an allergic patient
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Steinberger P, Kraft D, Valenta R: Construction of a combinatorial IgE library from an allergic patient J Biol Chem 1996, 271:10967-10972. Due to their low frequency within the expressed antibody repertoire, production of monoclonal IgE antibodies of known specificity had not been achieved previously. This study may lead to advances in the treatment of allergy.
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(1996)
J Biol Chem
, vol.271
, pp. 10967-10972
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Steinberger, P.1
Kraft, D.2
Valenta, R.3
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21
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0029908633
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Isolation of a neutralizing human RSV antibody from a dominant, non-neutralizing immune repertoire by epitope-blocked panning
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Tsui P, Tometta MA, Ames RS, Bankosky BC, Griego S, Silverman C, Porter T, Moore G, Sweet RW: Isolation of a neutralizing human RSV antibody from a dominant, non-neutralizing immune repertoire by epitope-blocked panning. J Immunol 1996, 157:772-780. Often selection from libraries can lead to the isolation of antibodies directed against a dominant epitope. By blocking a dominant epitope with a previously isolated antibody and repanning, antibodies against rare epitopes may be recovered. This study confirms the efficacy of the epitope masking procedure described earlier in studies of anti-HIV-1 antibody selection.
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(1996)
J Immunol
, vol.157
, pp. 772-780
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Tsui, P.1
Tometta, M.A.2
Ames, R.S.3
Bankosky, B.C.4
Griego, S.5
Silverman, C.6
Porter, T.7
Moore, G.8
Sweet, R.W.9
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22
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0028941198
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Chimeric macaque/human Fab molecules neutralize simian immunodeficiency virus
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Samuelsson A, Chiodi F, Ohman P, Putkonen P, Norrby E, Persson MAA: Chimeric macaque/human Fab molecules neutralize simian immunodeficiency virus. Virology 1995, 207:495-502.
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(1995)
Virology
, vol.207
, pp. 495-502
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Samuelsson, A.1
Chiodi, F.2
Ohman, P.3
Putkonen, P.4
Norrby, E.5
Persson, M.A.A.6
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23
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0029962226
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A recombinant antibody with the antigen-specific, major histocompatibility complex-restricted specificity of T cells
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Andersen PS, Stryhn A, Hansen BE, Fugger L, Engberg J, Buus S: A recombinant antibody with the antigen-specific, major histocompatibility complex-restricted specificity of T cells. Proc Natl Acad Sci USA 1996, 93:1820-1824. A mouse Fab that binds selectively to a mouse peptide-MHC complex was selected by panning a Fab library derived from immune mice. Antibodies specific for peptide-MHC complexes will be important diagnostic and ther-apeutic tools.
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(1996)
Proc Natl Acad Sci USA
, vol.93
, pp. 1820-1824
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Andersen, P.S.1
Stryhn, A.2
Hansen, B.E.3
Fugger, L.4
Engberg, J.5
Buus, S.6
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24
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0029867492
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Recombinant Phabs reactive with 7, 8-dihydro-8-oxoguanine, a major oxidative DNa lesion
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Bespalov IA, Purmal AA, Glackin MP, Wallace SS, Melamede RJ: Recombinant Phabs reactive with 7, 8-dihydro-8-oxoguanine, a major oxidative DNA lesion. Biochemistry 1996, 35:2067-2078.
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(1996)
Biochemistry
, vol.35
, pp. 2067-2078
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Bespalov, I.A.1
Purmal, A.A.2
Glackin, M.P.3
Wallace, S.S.4
Melamede, R.J.5
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25
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0029901753
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High affinity antibodies against Lex and sialyl Lex from a phage display library
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Dinh Q, Weng NP, Kiso M, Ishida H, Hasegawa A, Marcus DM: High affinity antibodies against Lex and sialyl Lex from a phage display library. J Immunol 1996, 157:732-738. High-affinity anticarbohydrate antibodies have been thought to be rare, if not impossible, to produce. This report demonstrates that selection allows for the rapid isolation of this interesting class of antibodies.
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(1996)
J Immunol
, vol.157
, pp. 732-738
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Dinh, Q.1
Weng, N.P.2
Kiso, M.3
Ishida, H.4
Hasegawa, A.5
Marcus, D.M.6
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26
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0029857998
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Recombinant immunotoxins specific for a mutant epidermal growth factor receptor: Targeting with a single chain antibody variable domain isolated by phage display
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Lorimer IA, Keppler-Hafkemeyer A, Beers RA, Pegram CN, Bigner DD, Pastan I: Recombinant immunotoxins specific for a mutant epidermal growth factor receptor: targeting with a single chain antibody variable domain isolated by phage display. Proc Natl Acad Sci USA 1996, 93:14815-14820. A mouse scFv that binds to a mutant human epidermal growth factor receptor was selected by panning an scFv library derived from mice immunized with a synthetic peptide. An immunotoxin produced by fusing the selected scFv to Pseudomonas exotoxin A revealed good affinity, cytotoxicity, and stability.
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(1996)
Proc Natl Acad Sci USA
, vol.93
, pp. 14815-14820
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Lorimer, I.A.1
Keppler-Hafkemeyer, A.2
Beers, R.A.3
Pegram, C.N.4
Bigner, D.D.5
Pastan, I.6
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27
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0029757498
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Phage diabody repertoires for selection of large numbers of bispecific antibody fragments
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McGuinness BT, Walter G, FitzGerald K, Schuler P, Mahoney W, Duncan AR, Hoogenboom HR: Phage diabody repertoires for selection of large numbers of bispecific antibody fragments. Nat Biotechnol 1996, 14:1149-1154. Study of a model system for the selection of dimeric scFvs that possess binding sites with two unique specificities. This technique holds potential for the development of antibodies that cross-link molecules on the cell surface or act as molecular adhesives.
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(1996)
Nat Biotechnol
, vol.14
, pp. 1149-1154
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McGuinness, B.T.1
Walter, G.2
Fitzgerald, K.3
Schuler, P.4
Mahoney, W.5
Duncan, A.R.6
Hoogenboom, H.R.7
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28
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0029955258
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Selection of linkers for a catalytic single-chain antibody using phage display technology
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Tang Y, Jiang N, Parakh C, Hilvert D: Selection of linkers for a catalytic single-chain antibody using phage display technology. J Biol Chem 1996, 271:15682-15686. Expression of antibodies in the scFv format is often an unsuccessful undertaking. This report demonstrates that selection of the linker sequence connecting the variable region fragments affects both affinity for antigen and the level of scFv expression.
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(1996)
J Biol Chem
, vol.271
, pp. 15682-15686
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Tang, Y.1
Jiang, N.2
Parakh, C.3
Hilvert, D.4
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29
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0030013173
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Circumventing tolerance to generate autologous monoclonal antibodies to the prion protein
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Williamson RA, Peretz D, Smorodinsky N, Bastidas R, Serban H, Mehlhom I, DeArmond SJ, Prusiner SB, Burton DR: Circumventing tolerance to generate autologous monoclonal antibodies to the prion protein. Proc Natl Acad Sci USA 1996, 93:7279-7282. Construction of combinatorial libraries from prion knockout mice and immunization with prion protein enables the first selection of high-affinity antibodies to this important protein. This strategy may be an important one for the generation of antibodies to highly conserved proteins.
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(1996)
Proc Natl Acad Sci USA
, vol.93
, pp. 7279-7282
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Williamson, R.A.1
Peretz, D.2
Smorodinsky, N.3
Bastidas, R.4
Serban, H.5
Mehlhom, I.6
DeArmond, S.J.7
Prusiner, S.B.8
Burton, D.R.9
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30
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0029950801
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Recombinant rabbit Fab with binding activity to type-1 plasminogen activator inhibitor derived from a phage-display library against human alpha-granules
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Lang IM, Barbas CF III, Schleef RR: Recombinant rabbit Fab with binding activity to type-1 plasminogen activator inhibitor derived from a phage-display library against human alpha-granules. Gene 1996, 172:295-298.
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(1996)
Gene
, vol.172
, pp. 295-298
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Lang, I.M.1
Barbas C.F. III2
Schleef, R.R.3
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31
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0030210311
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Chicken monoclonal antibody isolated by a phage display system
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L gene which is diversified via gene conversion. Primers are available for cloning chicken antibodies in the pComb3H phagemid vector.
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(1996)
J Immunol
, vol.157
, pp. 1156-1162
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Yamanaka, H.I.1
Inoue, T.2
Ikeda-Tanaka, O.3
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32
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0029802551
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Phage antibodies with pan-species recognition of the oncofetal angiogenesis marker fibronectin ED-B domain
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Carnemolla B, Neri D, Castellani P, Leprini A, Neri G, Pini A, Winter G, Zardi L: Phage antibodies with pan-species recognition of the oncofetal angiogenesis marker fibronectin ED-B domain. Int J Cancer 1996, 68:397-405.
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(1996)
Int J Cancer
, vol.68
, pp. 397-405
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Carnemolla, B.1
Neri, D.2
Castellani, P.3
Leprini, A.4
Neri, G.5
Pini, A.6
Winter, G.7
Zardi, L.8
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33
-
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0029887264
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Microtubule minus ends can be labelled with a phage display antibody specific to alpha-tubulin
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Fan J, Griffiths AD, Lockhart A, Cross RA, Amos LA: Microtubule minus ends can be labelled with a phage display antibody specific to alpha-tubulin. J Mol Biol 1996, 259:325-330.
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(1996)
J Mol Biol
, vol.259
, pp. 325-330
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Fan, J.1
Griffiths, A.D.2
Lockhart, A.3
Cross, R.A.4
Amos, L.A.5
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34
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0030570722
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A model phage display subtraction method with potential for analysis of differential gene expression
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Stausbol-Gron B, Wind T, Kjaer S, Kahns L, Hansen NJV, Kristensen P, Clark BFC: A model phage display subtraction method with potential for analysis of differential gene expression. FEBS Lett 1996, 391:71-75.
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(1996)
FEBS Lett
, vol.391
, pp. 71-75
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Stausbol-Gron, B.1
Wind, T.2
Kjaer, S.3
Kahns, L.4
Hansen, N.J.V.5
Kristensen, P.6
Clark, B.F.C.7
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35
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9344223986
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Human antibodies with sub-nanomolar affinities isolated from a large non-immunized phage display library
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Vaughan TJ, Williams AJ, Pritchard K, Osboum JK, Pope AR, Earnshaw JC, McCafferty J, Hodits RA, Wilton J, Johnson KS: Human antibodies with sub-nanomolar affinities isolated from a large non-immunized phage display library. Nat Biotechnol 1996, 14:309-314. Antibodies of good affinity may be directly selected from large repertoires of V genes. This report involves the selection of scFv fragments which often aggregate, leading to an overestimate of their monovalent binding affinity. A general rule in combinatorial antibody selection is if your application demands a scFv, select a scFv. If your application requires a Fab or a whole antibody, select from a Fab library. Antibodies selected as scFv do not generally bind their antigen well when they are converted for expression in the Fab or whole antibody format.
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(1996)
Nat Biotechnol
, vol.14
, pp. 309-314
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Vaughan, T.J.1
Williams, A.J.2
Pritchard, K.3
Osboum, J.K.4
Pope, A.R.5
Earnshaw, J.C.6
McCafferty, J.7
Hodits, R.A.8
Wilton, J.9
Johnson, K.S.10
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36
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0029861453
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In vitro antigen challenge of human antibody libraries for vaccine evaluation: The human immunodeficiency virus type 1 envelope
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Parren PWHI, Fisicaro P, Labrijn AF, Binley JM, Yang WP, Ditzel HJ, Barbas CF III, Burton DR: In vitro antigen challenge of human antibody libraries for vaccine evaluation: the human immunodeficiency virus type 1 envelope. J Virol 1996, 70:9046-9050. Based on the HIV-1 -neutralizing ability of human Fab that were selected by panning a Fab library derived from a HIV-1 positive individual on different preparations of the HIV-1 envelope glycoprotein, the authors suggest that the ability of antigen preparations to select neutralizing antibodies from combinatorial antibody libraries could be used as a measure of the quality of the antigen as a vaccine.
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(1996)
J Virol
, vol.70
, pp. 9046-9050
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-
Parren, P.W.H.I.1
Fisicaro, P.2
Labrijn, A.F.3
Binley, J.M.4
Yang, W.P.5
Ditzel, H.J.6
Barbas C.F. III7
Burton, D.R.8
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37
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0029932458
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Organ targeting in vivo using phage display peptide libraries
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Pasqualini R, Ruoslahti E: Organ targeting in vivo using phage display peptide libraries. Nature 1996, 380:364-366. Peptides that home to target organs were selected by in vivo panning of phage displayed peptide libraries, a technique that should also be feasible with phage displayed antibody libraries. Selective in vivo targeting by peptides or antibodies promises applications in gene and cancer therapy.
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(1996)
Nature
, vol.380
, pp. 364-366
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Pasqualini, R.1
Ruoslahti, E.2
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38
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0031017133
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Designing therapies that target tumor blood vessels
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Barinaga M: Designing therapies that target tumor blood vessels. Science 1997, 275:482-484.
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(1997)
Science
, vol.275
, pp. 482-484
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Barinaga, M.1
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39
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0030575802
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Isolation of picomolar affinity anti-c-erbB-2 single-chain Fv by molecular evolution of the complementarity determining regions in the center of the antibody binding site
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Schier R, McCall A, Adams GP, Marshall KW, Merritt H, Yim M, Crawford RS, Weiner LM, Marks C, Marks JD: Isolation of picomolar affinity anti-c-erbB-2 single-chain Fv by molecular evolution of the complementarity determining regions in the center of the antibody binding site. J Mol Biol 1996, 263:551-567. CDR walking mutagenesis focused on LCDR3 and HCDR3 leads to large gains in affinity for this therapeutically interesting scFv. See also [6] for a comprehensive investigation of the CDR walking mutagenesis strategy as it relates to additivity within antibody combining sites. Currently, this strategy has led to the greatest success in improving the affinity of already high affinity antibodies.
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(1996)
J Mol Biol
, vol.263
, pp. 551-567
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Schier, R.1
McCall, A.2
Adams, G.P.3
Marshall, K.W.4
Merritt, H.5
Yim, M.6
Crawford, R.S.7
Weiner, L.M.8
Marks, C.9
Marks, J.D.10
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40
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0028845856
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Contribution of antibody heavy chain CDR1 to digoxin binding analyzed by random mutagenesis of phage-displayed Fab 26-10
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Short MK, Jeffrey PD, Kwong RF, Margolies MN: Contribution of antibody heavy chain CDR1 to digoxin binding analyzed by random mutagenesis of phage-displayed Fab 26-10. J Biol Chem 1995, 270:28541-28550.
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(1995)
J Biol Chem
, vol.270
, pp. 28541-28550
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Short, M.K.1
Jeffrey, P.D.2
Kwong, R.F.3
Margolies, M.N.4
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41
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0029927859
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Affinity maturation of a high-affinity human monoclonal antibody against the third hypervariable loop of human immunodeficiency virus: Use of phage display to improve affinity and broaden strain reactivity
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Light chain shuffling of a high affinity antibody results in a drift in epitope recognition
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Isolation of high-affinity monomeric human anti-c-erbB-2 single chain Fv using affinity-driven selection
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Schier R, Bye J, Apell G, McCall A, Adams GP, Malmqvist M, Weiner LM, Marks JD: Isolation of high-affinity monomeric human anti-c-erbB-2 single chain Fv using affinity-driven selection. J Mol Biol 1996, 255:28-43.
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Evidence for distinct contributions of heavy and light chains to restriction of antibody recognition of the HIV-1 principal neutralization determinant
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Watkins BA, Davis AE, Fiorentini S, di MarzoVeronese F, Reitz MS Jr: Evidence for distinct contributions of heavy and light chains to restriction of antibody recognition of the HIV-1 principal neutralization determinant J Immunol 1996, 156:1676-1683.
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Mimicking somatic hypermutation: Affinity maturation of antibodies displayed on bacteriophage using a bacterial mutator strain
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Low NM, Holliger P, Winter G: Mimicking somatic hypermutation: affinity maturation of antibodies displayed on bacteriophage using a bacterial mutator strain. J Mol Biol 1996, 260:359-368. By multiple rounds of growth in the mutator strain E. coli mutD5 followed by panning, the affinity of a human scFv to the hapten 2-phenyl-5-oxazolone was improved.
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Phage display vectors for in vivo recombination of immunoglobulin heavy and light chain genes to make large combinatorial libraries
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Tsurushita N, Fu H, Warren C: Phage display vectors for in vivo recombination of immunoglobulin heavy and light chain genes to make large combinatorial libraries. Gene 1996, 172:59-63.
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Co-selection of cognate antibody-antigen pairs by selectively-infective phages
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