Complementary combining site contact residue mutations of the anti-digoxin Fab 26-10 permit high affinity wild-type binding

Journal of Biological Chemistry

Volumn 277, Issue 19, 2002, Pages 16365-16370

  Short, Mary K ^a   Krykbaev, Rustem A ^b   Jeffrey, Philip D ^c   Margolies, Michael N ^a,c,d  

^a MASSACHUSETTS GENERAL HOSPITAL   (United States)

^b MEMORIAL SLOAN KETTERING CANCER CENTER   (United States)

^c CINCINNATI CHILDREN'S HOSPITAL MEDICAL CENTER   (United States)

^d PFIZER INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODIES; BACTERIOPHAGES; CLONING; MUTAGENESIS; SOLUTIONS;

CHAIN RESIDUES;

BIOCHEMISTRY;

16 ACETYLGITOXIN; ARGININE; ASPARAGINE; BOVINE SERUM ALBUMIN; CARDENOLIDE; DIGOXIN; DIGOXIN ANTIBODY F(AB) FRAGMENT; GITOXIN; HAPTEN; PLANT GLYCOSIDE; UNCLASSIFIED DRUG; VALINE;

AMINO ACID SEQUENCE; ANTIBODY STRUCTURE; ARTICLE; BINDING AFFINITY; BINDING SITE; CONTROLLED STUDY; GENE MUTATION; MOLECULAR CLONING; NONHUMAN; PHAGE DISPLAY; PRIORITY JOURNAL; SECONDARY IMMUNE RESPONSE;

ACETYLDIGOXINS; ANIMALS; ANTIBODIES, MONOCLONAL; ARGININE; BINDING SITES; CRYSTALLOGRAPHY, X-RAY; DIGOXIN; ENZYME-LINKED IMMUNOSORBENT ASSAY; GENETIC VECTORS; HAPTENS; KINETICS; MICE; MODELS, CHEMICAL; MUTAGENESIS, SITE-DIRECTED; MUTATION; PROTEIN BINDING; SERUM ALBUMIN; VALINE;

BOVINAE; UNIDENTIFIED BACTERIOPHAGE;

EID: 0037053332     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M110444200     Document Type: Article

References (25)