Specificity improvement of a recombinant anti-testosterone Fab fragment by CDRIII mutagenesis and phage display selection

Protein Engineering

Volumn 11, Issue 4, 1998, Pages 311-319

  Hemminki, A ^a   Niemi, S ^b   Hoffren A M ^c,d   Hakalahti, L ^b   Soderlund H ^a   Takkinen, K ^a  

^a VTT TECHNICAL RESEARCH CENTRE OF FINLAND   (Finland)

^b Orion Diagnostica Oy   (Finland)

^c ÅBO AKADEMI UNIVERSITY   (Finland)

^d Oy Juvantia Pharma Ltd   (Finland)

Author keywords

CDRIII mutagenesis; Molecular modelling; Phage display; Recombinant Fab; Specificity improvement

Indexed keywords

IMMUNOGLOBULIN F(AB) FRAGMENT; IMMUNOGLOBULIN HEAVY CHAIN; IMMUNOGLOBULIN LIGHT CHAIN; MONOCLONAL ANTIBODY; PRASTERONE SULFATE; TESTOSTERONE;

ANTIBODY AFFINITY; ARTICLE; COMPLEMENTARITY DETERMINING REGION; CROSS REACTION; IMMUNOASSAY; MOLECULAR MODEL; MUTAGENESIS; PHAGE DISPLAY; PRIORITY JOURNAL;

EID: 0031800681     PISSN: 02692139     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/11.4.311     Document Type: Article

References (77)

1. Alfthan, K. et al. (1993) Gene, 128, 203-209.
2. Allen, F.H. et al. (1991) J. Chem. Inf. Comput. Sci., 31, 187-204.
3. Alzari, P.M., Spinelli, S., Mariuzza, R.A., Boulot, G., Poljak, R.J., Jarvis, J.M. and Milstein, C. (1990) EMBO J., 9, 3807-3814.
4. Arevalo, J.H., Stura, E.A., Taussig, M.J. and Wilson, I.A. (1993a) J. Mol. Biol., 231, 103-118.
5. Arevalo, J.H., Taussig, M.J. and Wilson, I.A. (1993b) Nature, 365, 859-863.
6. Arevalo, J.H., Hassig, C.A., Stura, E.A., Sims, M.J., Taussig, M.J. and Wilson, I.A. (1994) J. Mol. Biol., 241, 663-690.
7. Barbas, C.F., III., Kang A.S., Lerner R.A. and Benkovic S.J. (1991) Proc. Natl Acad. Sci. USA, 88, 7978-7982.
8. Barbas, C.F., III, Hu, D., Dunlop, N., Sawyer, L., Cababa, D., Hendry, R.M., Nara, P.L. and Burton, D.R. (1994) Proc. Natl Acad. Sci. USA, 91, 3809-3813.
9. Baumann, M. (1990) Anal. Biochem., 190, 198-208.
10. Berek, C. and Milstein, C. (1987) Immunol. Rev., 105, 5-23.
11. Berkowitz, M. and McCammon, J.A. (1982) Chem. Phys. Lett., 90, 215-217.
12. Bemstein, F.C. et al (1977) J. Mol. Biol., 112, 535-542.
13. Better, M., Chang, C.P., Robinson, R.R. and Horwitz, A.H. (1988) Science, 240, 1041-1043.
14. Blundell, T.L. et al. (1988) Eur. J. Biochem., 172, 513-520.
15. Blundell, T.L., Johnson, M.S., Overington, J.P. and Sali, A. (1990) In Hook, J.B. and Poste, G. (eds), Protein Design and the Development of New Therapeutics and Vaccines. Plenum Press, New York, pp. 209-227.
16. Braunwald, E., Isselbacher, K.J., Petersdorf, R.G., Wilson, J.D., Martin, J.B. and Fauci, A.S. (eds) (1987) Harrison's Principles of Internal Medicine 2. McGraw-Hill, New York.
17. Brooks, C.L., III and Karplus, M. (1989) J. Mol. Biol., 208, 159-181.
18. Brooks, C.L., III, Karplus, M. and Pettit, B.M. (1988) Adv. Chem. Phys., 71, 33-58.
19. Brunger, A.T., Brooks, C.L., III and Karplus, M. (1985) Proc. Natl Acad. Sci. USA, 82, 8458-8462.
20. Carter, P. et al. (1992) Bio/Technology, 10, 163-167.
21. Deng, S.-J., MacKenzie, C.R., Himara, T., Brousseau, R., Lowary, T.L., Young, N.M., Bundle, D.R. and Narang, S.A. (1995) Proc. Natl Acad. Sci. USA, 92, 4992-4996.
22. Franek, M. (1987) J. Steroid Biochem., 28, 95-108.
23. Griffiths, A.D. et al. (1993) EMBO J., 12, 725-734.
24. Harlow, E. and Lane, D. (1988) Antibodies, A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
25. Hawkins, R.E. and Winter, G. (1992) Eur. J. Immunol., 22, 867-870.
26. Hermes, J.D., Parekh, S.M., Blacklow, S.C., Köster, H. and Knowles, J.R. (1989) Gene, 84, 143-151.
27. Hermes, J.D., Blacklow, S.C. and Knowles, J.R. (1990) ) Proc. Natl Acad. Sci. USA, 87, 696-700.
28. Herron, J.N., He, X., Mason, M.L., Voss, W., Jr and Edmundson, A.B. (1989) Proteins, 5, 271.
29. Ho, S.N., Hunt, H.D., Horton, R.M., Pullen, J.K. and Pease, L.R. (1989) Gene, 77, 51-59.
30. Hoffrén, A.-M., Holm, L., Laaksonen, L., Teeri, T.T. and Teleman, O. (1992) Immuno Methods, 1, 80-90.
31. Holliger, P., Prospero, T. and Winter, G. (1993) Proc. Natl Acad. Sci. USA, 90, 6444-6448.
32. Ismail, A.A.A., Astley, P., Cawood, M., Short, F., Wakelin, K. and Wheeler, M. (1986) Ann. Clin. Biochem., 23, 135-145.
33. Jeffrey, P.D., Strong, R.K., Sieker, L.C. Chang, C.Y.Y., Cambell, R.L., Petsko, G.A., Haber, E., Margolies, M.N. and Sheriff, S. (1993) Proc. Natl Acad. Sci. USA, 90, 10310-10314.
34. Johnson, M.S. and Overington, J.P. (1993) J. Mol. Biol., 233, 716-738.
35. Johnson, M.S., Overington, J.P. and Blundell, T.L. (1993) J. Mol. Biol., 231, 735-752.
36. Johnsson, B., Löfas, S. and Lindqvist, G. (1991) Anal. Biochem., 198, 268-277.
37. Jonsson, U. et al. (1991) BioTecliniques, 11, 620-627.
38. Karlsson, R., Michaelsson, A. and Mattson, A. (1991) J. Immunol. Methods, 145, 229-240.
39. Kabat, E.A., Wu, T.T., Reid-Miller, M., Perry, H.M. and Gottesman, K.S. (1991) Sequences of Proteins of Immunological Interest. 4th edn. US Department of Health and Human Services, Bethesda, MD.
40. Korth-Schutz, S., Virdis, R., Saenger, P., Chow, D.M., Levine, L.S. and New, M.I. (1978) J. Clin. Endocrinol. Metab., 46, 452-457.
41. Laemmli, U.K. (1970) Nature, 227, 680-685.
42. Lei, S.-P., Lin, H.-C., Wang, S.-S., Callaway, J. and Wilcox, G. (1987) J. Bacteriol., 169, 4379-4383.
43. Leung, D.W., Chen, E. and Goeddel, D.V., (1989) Tech. J. Methods Cell Mol. Biol., 1, 11.
44. MacKenzie, C.R., Sharma, V., Brummell, D., Bilous, D., Dubuc, G., Sadowska, J., Young, N.M., Bundle, D.R. and Narang, S.A. (1994) Bio/Technology, 12, 390-395.
45. Marks, J.D., Hoogenboom H.R., Bonnert, T.P., McCafferty, J., Griffiths, A.D. and Winter, G. (1991) J. Mol. Biol., 222, 581-597.
46. McCafferty, J., Griffiths, A.D., Winter, G. and Chiswell, F.J. (1990) Nature, 348, 552-554.
47. Myers, R.M., Lerman, L.S. and Maniatis, T. (1985) Science, 229, 242-247.
48. Odel, W.D. and Swerdloff, R.S. (1978) Clin. Endocrinol., 8, 149-180.
49. Orlandi, R., Gussow, D.H., Jones, P.T. and Winter, G. (1989) Proc. Natl Acad. Sci. USA, 86, 3833-3837.
50. Osborn, R.H. and Yannone, M.E. (1973) Gynecol. Surv., 26, 195-228.
51. Parham, P., Androlewicz, M.J., Brodsky, F.M., Holmes, N.J. and Ways, J.P. (1982) J. Immunol. Methods, 53, 133-173.
52. Pope, A., Pritchard, K., Williams, A., Roberts, A., Hackett, J.R., Mandecki, W. and Johnson, K.S. (1996) Immunotechnology, 2, 209-217.
53. Saiki, R.K., Gelfand, D.H., Stoffel, S., Scharf, S.J., Higuchi, R., Horn, G.T., Mullis, K.B. and Erlich, H.A (1988) Science, 239, 487-491.
54. Sali, A., Overington, J.P., Johnson, M.S. and Blundell, T.L. (1990) Trends Biochem. Sci., 15, 235-240.
55. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1990) Molecular Cloning. A Laboratory Manual. 2nd edn., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
56. Sanger, F., Nicklen, S. and Coulson, A.R. (1977) Proc. Natl Acad. Sci. USA, 74, 5463-5467.
57. Schaaper, R.M. (1988) Proc. Natl Acad. Sci. USA, 85, 8126-8130.
58. Schier, R., Bye, J.M., Apell, G., McCall, A., Adams, G.P., Malmqvist, M., Weiner, L.M. and Marks, J.D. (1996a) J. Mol. Biol., 225, 28-43.
59. Schier, R., Balint, R.F., McCall, A., Apell, G., Larrick, J.W. and Marks, J.D. (1996b) Gene, 169, 147-155.
60. Schier, R. et al (1996e) J. Mol Biol., 263, 551-567.
61. Schildbach, J.F. et al. (1994) Protein Sci., 3, 737-749.
62. Short, M.K., Jeffrey, P.D., Kwong, R.-F. and Margolies, M.N. (1995) J. Biol. Chem., 270, 28541-28550.
63. Skerra, A. and Pluckthun, A., (1988) Science, 240, 1038-1041.
64. Sutcliffe, M.J., Haneef, I., Carney, D. and Blundell, T.L. (1987a) Protein Engng, 1, 377-384.
65. Sutcliffe, M.J., Hayes, F.R.F. and Blundell, T.L. (1987b) Protein Engng, 1, 385-392.
66. Takkinen, K., Laukkanen, M.-L., Sizmann, D., Alfthan, K., Immonen, T., Vanne, L., Kaartinen, M., Knowles, J.K.C. and Teeri, T.T. (1991) Protein Engng, 4, 837-841.
67. Tieffenaur, L.X., Bodmer, D.M., Frei, W. and Andres, R.Y. (1989) J. Steroid Biochem., 32, 251-257.
68. Towbin, H., Staehlin, T. and Gordon, J. (1979) Proc. Natl Acad. Sci. USA, 76, 4350-4354.
69. Wang, C. and Swedloff, R.S. (1992) Baillieres Clin. Endocrinol Metab., 6, 405-4354.
70. Wetlaufer, D. (1962) Adv. Protein Chem., 17, 303-390.
71. Wheeler, M.J. (1994) In: Wild, D. (ed.) The Immunoassay Handbook. Macmillan, Basingstoke, pp. 365-378.
72. Whitlow, M., Filpula, D., Rollence, M.L., Feng, S.L. and Wood, J.F. (1994) Protein Engng, 7, 1017-1026.
73. Wilke, T.J. and Utley, D.J. (1987) Clin. Chem., 33, 1372-1375.
74. Wilson, I.A. and Stanfield, R.L. (1994) Curr. Opin. Struct. Biol. 4, 857-867.
75. Wu, F.C.W., Edmond, P., Raap, G. and Hunter, W.M. (1981) Clin. Endocrinol., 14, 493-507.
76. Yang, W.-P., Green, K., Pinz-Sweeney, S., Briones, A.T., Burton, D.R. and Barbas, C.F. (1995) J. Mol. Biol., 254, 392-403.
77. Yelton, D.E., Rosok, M.J., Cruz, G., Cosand, W.L., Bajorath, J., Hellström, I., Hellstrom, K.E., Huse, W.D. and Glaser, S.M. (1995) J. Immunol., 155, 1994-2004.