Metabolic engineering of Lactococcus lactis: The impact of genomics and metabolic modelling

Journal of Biotechnology

Volumn 98, Issue 2-3, 2002, Pages 199-213

  Kleerebezem, Michiel ^a   Boels, Ingeborg C ^a   Groot, Masja Nierop ^a   Mierau, Igor ^a   Sybesma, Wilbert ^a   Hugenholtz, Jeroen ^a  

^a NIZO FOOD RESEARCH   (Netherlands)

Author keywords

Genomics; Lactic acid bacteria; Lactococcus lactis; Metabolic engineering; Pyruvate metabolism

Indexed keywords

BACTERIA; GENES; METABOLISM; ORGANIC ACIDS; SUGARS;

BIOSYNTHETIC PATHWAYS;

BIOTECHNOLOGY;

2,3 BUTANEDIONE; ALANINE; FOLIC ACID; LACTIC ACID; POLYSACCHARIDE; PYRUVIC ACID; SUGAR;

BACTERIAL METABOLISM; BIOSYNTHESIS; BIOTECHNOLOGY; CONFERENCE PAPER; GENETIC ENGINEERING; GENETIC MODEL; GENOMICS; LACTIC ACID BACTERIUM; LACTOBACILLUS DELBRUECKII; METABOLIC ENGINEERING; METHODOLOGY; NONHUMAN; PRIORITY JOURNAL;

FERMENTATION; FOLIC ACID; GENETIC ENGINEERING; GENOMICS; LACTOCOCCUS LACTIS; MODELS, CHEMICAL; MODELS, GENETIC; MODELS, MOLECULAR; POLYSACCHARIDES, BACTERIAL; PYRUVIC ACID;

LACTOCOCCUS; LACTOCOCCUS LACTIS;

EID: 0037174290     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(02)00132-3     Document Type: Conference Paper

References (69)

1. Effect of fermentation on B-vitamin content of milk in Sweden
2. Micronutrient deficiencies cause DNA damage and cancer
3. Xanthan gum biosynthesis and application: A biochemical/genetic perspective
4. Inhibition by selected food components of human and porcine intestinal pteroylpolyglutamate hydrolase activity
5. Metabolic engineering of exopolysaccharide production in Lactococcus lactis
6. Functional analysis of the Lactococcus lactis galU and galE genes and their impact on sugar nucleotide and exopolysaccharide biosynthesis
7. The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403
8. A quantitative assessment of plasma homocysteine as a risk factor for vascular disease
9. Vitamins as homocysteine-lowering agents
10. Improved vectors for nisin-controlled expression in gram-positive bacteria
11. Metabolic modeling of microbial strains in silico
12. Determination of full 13C isotopomer distributions for metabolic flux analysis using heteronuclear spin echo difference NMR spectroscopy
13. Controlled gene expression systems for Lactococcus lactis with the food-grade inducer nisin
14. Heteropolysaccharides from lactic acid bacteria
15. Metabolic engineering of sugar catabolism in lactic acid bacteria
16. Use of the lactococcal nisA promoter to regulate gene expression in Gram-positive bacteria: Comparison of induction level and promoter strength
17. Assessing evolutionary relationships among microbes from whole genome analysis
18. Comparative genomics and understanding of microbial biology
19. Microbial genome sequencing
20. + ratio
21. Dietary modulation of the human colonic microbiota: Introducing the concept of prebiotics
22. Heterologous expression of an immunogenic pneumococcal type 3 capsular polysaccharide in Lactococus lactis
23. Genetics of galactose utilisation via the Leloir pathway in lactic acid bacteria
24. Role of enzyme-bound 5,10-methenyltetrahydropteroylpolyglutamate in catalysis by Escherichia coli DNA photolyase
25. Metabolic engineering of lactic acid bacteria, the combined approach: Kinetic modelling, metabolic control and experimental analysis
26. Conversion of Lactococcus lactis from homolactic to homoalanine fermentation through metabolic engineering
27. Acetate utilization in Lactococcus lactis deficient in lactate dehydrogenase: A rescue pathway for maintaining redox balance
28. Characterization of a water-soluble polysaccharide fraction with immunopotentiating activity from Bifidobacterium adolescentis M101-4
29. Metabolic engineering of Lactococcus lactis leading to high diacetyl production
30. Antitumoral activity of slime-froming, encapsulated Lactococus lactis subsp. cremoris isolated from Scandinavian ropy sour milk, "viili"
31. Metabolite and isotopomer balancing in the analysis of metabolic cycles: Theory
32. Exopolysaccharides produced by Lactococcus lactis: From genetic engineering to improved rheological properties?
33. Controlled gene expression systems for lactic acid bacteria: Transferable nisin inducible expression cassettes for Lactococcus, Leuconostoc and Lactobacillus spp.
34. Lactic acid bacteria as a cell factory: Rerouting of carbon metabolism in Lactococcus lactis by metabolic engineering
35. Quorum sensing-controlled gene expression in lactic acid bacteria
36. Requirement for phosphoglucomutase in exopolysaccharide biosynthesis in glucose- and lactose-utilizing Streptococcus thermophilus
37. Regulation of exopolysaccharide production by Lactococcus lactis subsp. cremoris by the sugar source
38. The role of NADH-oxidation in acetoin and diacetyl production from glucose in Lactococcus lactis
39. Cofactor engineering: A novel approach to metabolic engineering in Lactococcus lactis by controlled overexpression of NADH oxidase
40. Isolation and biochemical characterization of folate deficient mutants of Chinese hamster cells
41. Characterization of the locus encoding the Streptococcus pneumoniae type 19F capsular polysaccharide biosynthetic pathway
42. Cholesterol lowering activity of ropy fermented milk
43. Structure of the extracellular polysaccharide from slime-forming Lactococcus lactis subspecies cremoris SBT0495
44. Microbial genome sequencing 2000: New insights into physiology, evolution and expression analysis
45. Genome data: What do we learn
46. Metabolite and isotopomer balancing in the analysis of metabolic cycles: Applications
47. Adaptation of the nisin-controlled expression system in Lactobacillus plantarum: A tool to study in vivo biological effects
48. Relationship between glycolysis and exopolysaccharides biosynthesis in Lactococcus lactis
49. 13C-NMR studies of Cyanobacterium melassecola metabolic pathways
50. Inhibition of intestinal gamma-glutamyl carboxypeptidase by yeast nucleic acid: An explanation of variability in utilization of dietary polyglutamyl folate
51. Quantitative analysis of metabolic fluxes in Escherichia coli, using two-dimensional NMR spectroscopy and complete isotopomer models
52. Properties of food folates determined by stability and susceptibility to intestinal pteroylpolyglutamate hydrolase action
53. Increase of folate levels in fermented foods
54. Introduction of the exopolysaccharide gene cluster from Streptococcus thermophilus Sfi6 into Lactococcus lactis MG1363: Production and characterization of an altered polysaccharide
55. Novel and established applications of microbial polysaccharides
56. Improved efficiency and reliability of RT-PCR using tag-extended RT primers and temperature gradient PCR
57. Biological function of the dTDP-rhamnose synthetic pathway in Streptococcus mutans
58. Molecular characterization of the plasmid-encoded eps gene cluster essential for exopolysaccharide biosynthesis in Lactococcus lactis
59. Exopolysaccharide biosynthesis in Lactococcus lactis NIZO B40: Functional analysis of the glycosyltransferase genes involved in synthesis of the polysaccharide backbone
60. Functional analysis of glycosyltransferase genes from Lactococcus lactis and other Gram-positive cocci: Complementation, expression, and diversity
61. Nucleotide sequence analysis of the lactococcal EPS plasmid pNZ4000
62. Involvement of a plasmid in production of ropiness (mucoidness) in milk cultures by Streptococcus cremoris MS
63. Construction of Streptococcus lactis subsp. lactis strains with a single plasmid associated with mucoid phenotype
64. Prevention of neural tube defects. Results of the Medical Research Council vitamin study
65. From microbial genome sequence to application
66. Microbial genome analysis: Insights into virulence, host adaptation and evolution
67. Human gamma glutamyl hydrolase: Cloning and characterization of the enzyme expressed in vitro