Improved efficiency and reliability of RT-PCR using tag-extended RT primers and temperature gradient PCR

BioTechniques

Volumn 31, Issue 3, 2001, Pages 466-472

  Sybesma, W ^a   Hugenholtz, J ^a   Mierau, I ^a   Kleerebezem, M ^a  

^a NIZO FOOD RESEARCH   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CELL ISOLATION; DIAGNOSTIC ACCURACY; DNA DEGRADATION; DNA DETERMINATION; LACTOCOCCUS LACTIS; NONHUMAN; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TEMPERATURE SENSITIVITY;

EID: 0034856657     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/01313bm02     Document Type: Article

References (10)

1. Selective amplification of RNA utilizing the nucleotide analog dITP and Thermus thennophilus DNA polymerase
2. Use of manganese in RT-PCR eliminates PCR artifacts resulting from DNase I digestion
3. Low-redundancy sequencing of the entire Lactococcus lactis IL1403 genome
4. Selective RNA amplification: A novel method using dUMP-containing primers and uracil DNA glycosylase
5. Simultaneous detection of DNA and RNA by differential polymerase chain reaction (DIFF-PCR)
6. Evaluation of the effects of DNase treatment on signal specificity in RT-PCR and in situ RT-PCR
7. Selective amplification of bacterial RNA: Use of a DNA primer containing mismatched bases near its 3′ terminus to reduce false-positive signals
8. Characterization of the nisin gene cluster nisABTCIPR of Lactococcus lactis. Requirement of expression of the nisA and nis1 genes for development of immunity
9. RNA template-specific PCR: An improved method that dramatically reduces false positives in RT-PCR