Metabolite and isotopomer balancing in the analysis of metabolic cycles: II. Applications

Biotechnology and Bioengineering

Volumn 62, Issue 4, 1999, Pages 392-401

  Park, Sung M ^a   Klapa, Maria I ^a   Sinskey, Anthony J ^b   Stephanopoulos, Gregory ^a  

^a Massachusetts Institute of Technology Cambridge   (United States)

^b MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

13C NMR; GC MS; Isotopomer analysis; Metabolic flux analysis; TCA cycle

Indexed keywords

CARBOXYLIC ACIDS; ENZYMES; ESCHERICHIA COLI; GAS CHROMATOGRAPHY; ISOTOPES; MASS SPECTROMETRY; METABOLITES; MOLECULAR WEIGHT DISTRIBUTION; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; REACTION KINETICS; SUBSTRATES;

CARBOXYLASE; ISOTOPOMER ANALYSIS; METABOLIC FLUX ANALYSIS;

METABOLISM;

CITRIC ACID; PYRUVIC ACID;

ARTICLE; BACTERIAL METABOLISM; CITRIC ACID CYCLE; ESCHERICHIA COLI; ISOTOPE LABELING; MOLECULAR DYNAMICS; MOLECULAR MODEL; MOLECULAR WEIGHT;

ACETIC ACID; ACETONE; ANIMALS; BIOTECHNOLOGY; CARBON ISOTOPES; CITRATE (SI)-SYNTHASE; CITRIC ACID CYCLE; ESCHERICHIA COLI; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; GLYOXYLATES; MAGNETIC RESONANCE SPECTROSCOPY; MAMMALS; PROPIONIC ACIDS; PSEUDOMONAS; PYRUVATE CARBOXYLASE; PYRUVIC ACID;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MAMMALIA;

EID: 0033585823     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19990220)62:4<392::AID-BIT2>3.0.CO;2-S     Document Type: Article

References (31)

1. Antony GJ, Landau BR. 1968. Relative contribution of α, β-, and ω-oxidative pathways to in vitro fatty acid oxidation in rat liver. J Lipid Res 9:267-270.
2. 12 in the metabolism of propionate by the protozoan Ochromonas malhamensis. Biochem J 83:264-270.
3. 13C NMR study on fluxes into the TCA cycle of neuronal and glial tumor cell lines and primary cells. Biochimie 74:941-948.
4. Casazza JP, Felver ME, Veech RL. 1984. The metabolism of acetone in rat. J Biol Chem 259:231-236.
5. 13C NMR studies in perfused rat hearts. J Biol Chem 258:13785-13794.
6. 13C NMR study of effects of fasting and diabetes on the metabolism of pyruvate in the tricarboxylic acid cycle and of the utilization of pyruvate and ethanol in lipogenesis in perfused rat liver. Biochemistry 26:581-589.
7. 13C]acetate. Appl Environ Microbiol 53:2445-2451.
8. De Hertogh AA, Mayeux PA, Evans HJ. 1964. The relationship of cobalt requirement to propionate metabolism in Rhizohium. J Biol Chem 239:2446-2453.
9. 2 compounds: The involvement of glycollate in the metabolism of ethanol and of acetate by Pseudomonas AMI. Biochem J 128: 99-106.
10. 1 compounds. Biochem J 128:107-115.
11. 13C]Propionate oxidation in wild type and citrate synthase mutant Escherichia coli: Evidence for multiple pathways of propionate utilization. Biochem J 291:927-932.
12. Gavino VC, Sommas J, Philberts L, David F, Garneau M, Belair J, Brunengraher H. 1987. Production of acetone and conversion of acetone to acetate in the perfused rat liver. J Biol Chem 262:6735-6740.
13. 13C-NMR analysis of alanine metabolism by isolated perfused livers from C3HeB/FeJ mice infected with African trypanosomes. Comp Biochem Physiol 89B:679-685.
14. Hill RJ, Hobbs DC, Koeppe RE. 1958. The incorporation of noncarboxyl carbon into glutamic acid, alanine, and aspartic acid by the rat. J Biol Chem 230:169-178.
15. 13C]aspartic acid in renal epithelial cells. Biochim Biophys Acta 970: 241-250.
16. 13C-NMR study of glycerol metabolism in rabbit renal cells. Eur J Biochem 174:67-73.
17. 13C-NMR investigation of the metabolism of amino acids in renal proximal convoluted tubules of normal and streptozotocin-treated rats and rabbits. Biochem J 263:231-241.
18. Kam W, Kumaran K, Landau BR. 1978. Contribution of ω-oxidation to fatty acid oxidation by liver of rat and monkey. J Lipid Res 19: 591-600.
19. Klapa MI, Park SM, Sinskey AJ, Stephanopoulos GN. 1999. Metabolite and isotopomer balancing in the analysis of metabolic cycles: I. Theory Biotechnol Bioeng 62:375-391.
20. Kosugi K, Chandramouli V, Kumaran K, Schumann W, Landau BR. 1986. Determinants in the pathways followed by the carbons of acetone in their conversion to glucose. J Biol Chem 261:13179-13181.
21. 13C-NMR studies of acetate and methanol metabolism by methylotrophic Pseudomonas strains. J Gen Microbiol 135:1469-1477.
22. Owen OE, Trapp VE, Skatches CL, Mozzoli MA, Hoeldtke RD, Boden G, Reichard GA. 1982. Acetone metabolism during diabetic ketoacidosis. Diabetes 31:342-348.
23. Reichard GA, Haff AC, Skutches CL, Paul P, Holroyde CP, Owen OE. 1979. Plasma acetone metabolism in the fasting human. J Clin Invest 63:619-626.
24. Reichard GA, Skutches CL, Hoeldtke RD, Owen OE. 1986. Acetone metabolism in humans during diabetic ketoacidosis. Diabetes 35: 668-674.
25. Sakami W, Lafaye JM. 1951. The metabolism of acetone in the intact rat. J Biol Chem 193:199-203.
26. 14C]acetate and its use in assessing hepatic Krebs cycle activity and gluconeogenesis. J Biol Chem 266:6985-6990.
27. Sulway MJ, Malins JM. 1970. Acetone in diabetic ketoacidosis. Lancet 1:736-740.
28. 1H-NMR and biochemical methods. Eur J Biochem 197:271-279.
29. 15N]glutamate by Brevibacterium flavum. Appl Environ Microbiol 53:92-98.
30. Walsh K, Koshland DE. 1984. Determination of flux through the branch point of two metabolic cycles. The tricarboxylic acid cycle and the glyoxylate shunt. J Biol Chem 259:9646-9654.
31. Wood HG, Stjernholm R. 1961. Transcarboxylase: II. Purification and properties of methylmalonyloxaloacetic transcarboxylase. Proc Natl Acad Sci USA 47:289-303.