Human glioma cells and undifferentiated primary astrocytes that express aberrant EAAT2 mRNA inhibit normal EAAT2 protein expression and prevent cell death

Molecular and Cellular Neuroscience

Volumn 21, Issue 4, 2002, Pages 546-560

  Guo, Hong ^a   Lai, Liching ^a   Butchbach, Matthew E R ^a   Lin, Chien Liang Glenn ^a  

^a OHIO STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; GLUTAMATE TRANSPORTER; MESSENGER RNA; PROTEIN; PROTEIN EAAT2; UNCLASSIFIED DRUG;

ARTICLE; ASTROCYTE; CELL CLONE; CELL DEATH; CONTROLLED STUDY; CORRELATION ANALYSIS; GLIOMA CELL; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN DEPLETION; PROTEIN EXPRESSION;

EID: 0036939520     PISSN: 10447431     EISSN: None     Source Type: Journal    
DOI: 10.1006/mcne.2002.1198     Document Type: Article

References (42)

1. Abe, K., and Saito, H. (2000). L-Glutamate suppresses amyloid β-protein-induced stellation of cultured rat cortical astrocytes. J. Neurochem. 74: 280 -286.
2. Aoki, M., Lin, C. L. G., Rothstein, J. D., Geller, B. A., Hosler, B. A., Munsat, T. L., Horvitz, R., and Brown R. H., Jr. (1998). Mutations in the glutamate transporter EAAT2 gene do not cause abnormal EAAT2 transcripts in amyotrophic lateral sclerosis. Ann. Neurol. 43: 645-653.
3. Arriza, J. L., Eliasof, S., Kavanaugh, M. P., and Amara, S. G. (1997). Excitatory amino acid transporter 5, a retinal glutamate transporter coupled to a chloride conductance. Proc. Natl. Acad. Sci. USA 94: 4155-4160.
4. Arriza, J. L., Fairman, W. A., Wadiche, J. I., Murdoch, G. H., Kavanaugh, M. P., and Amara, S. G. (1994). Functional comparisons of three glutamate transporter subtypes cloned from human motor Cortex. J. Neurosci. 14: 5559-5569.
5. Bristol, L. A., and Rothstein, J. D. (1996). Glutamate transporter gene expression in amyotrophic lateral sclerosis. Ann. Neurol. 39: 676-679.
6. Carriedo, S. G., Yin, H. Z., Lamberta, R., and Weiss, J. H. (1995). In vitro kainate injury to large, SMI-32(+) spinal neurons is Ca2+ dependent. Neuroreport 6: 945-948.
7. Chen, C.-J., Liao, S.-L., and Kuo, J.-S. (2000). Gliotoxic action of glutamate on cultured astrocytes. J. Neurochem. 75: 1557-1565.
8. Chen, W., Aoki, C., Mahadomrongkul, V., Gruber, C. E., Wang, G. J., Blitzblau, R., Irwin, N., and Rosenberg, P. A. (2002). Expression of a variant form of the glutamate transporter GLT1 in neuronal cultures and in neurons and astrocytes in the rat brain. J. Neurosci. 22: 2142-2152.
9. Conti, F., DeBiasi, S., Minelli, A., Rothstein, J. D., and Melone, M. (1998). EAAC1, a high-affinity glutamate transporter, is localized to astrocytes and gabaergic neurons besides pyramidal cells in the rat cerebral cortex. Cereb. Cortex 8: 108-116.
10. Deng, X., Wang, Y., Chou, J., and Cadet, J. L. (2001). Methamphetamine causes widespread apoptosis in the mouse brain: Evidence from using an improved TUNEL histochemical method. Mol. Brain. Res. 93: 61-69.
11. Ebihara, K., Masuhiro, Y., Kitamoto, T., Suzawa, M., Uematsu, Y., Yoshizawa, T., Ono, T., Harada, H., Matsuda, K., Hasegawa, T., Masushige, S., and Kato, S. (1996). Intron retention generates a novel isoform of the murine vitamin D receptor that acts in a dominant negative way on the vitamin D signaling pathway. Mol. Cell. Biol. 16: 3393-3400.
12. Eng, D. L., Lee, Y. L., and Lal, P. G. (1997). Expression of glutamate uptake transporters after dibutyryl cyclic AMP differentiation and traumatic injury in cultured astrocytes. Brain Res. 778: 215-221.
13. Fairman, W. A., Vandenberg, R. J., Arriza, J. L., Kavanaugh, M. P., and Amara, S. G. (1995). An excitatory amino-acid transporter with properties of a ligand-gated chloride channel. Nature 375: 599-603.
14. Flowers, J. M., Powell, J. F., Leigh, P. N., Andersen, P., and Shaw, C. E. (2001). Intron 7 retention and exon 9 skipping EAAT2 mRNA variants are not associated with amyotrophic lateral sclerosis. Ann. Neurol. 49: 643-649.
15. Furuta, A., Martin, L. J., Lin, C. L. G., Dykes-Hoberg, M., and Rothstein, J. D. (1997). Cellular and synaptic localization of the neuronal glutamate transporters excitatory amino acid transporter 3 and 4. Neuroscience 81: 1031-1042.
16. Gavrieli, Y., Sherman, Y., and Ben-Sasson, S. A. (1992). Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J. Cell. Biol. 119: 493-501.
17. Gegelashvili, G., Danbolt, N. C., and Schousboe, A. (1997). Neuronal soluble factors differentially regulate the expression of GLT1 and GLAST glutamate transporters in cultured astroglia. J. Neurochem. 69: 2612-2615.
18. Haugeto, Ø., Ullensvang, K., Levy, L. M., Chaudhry, F. A., Honoré, T., Nielsen, M., Lehre, K. P., and Danbolt, N. C. (1996). Brain glutamate transporter proteins form homomultimers. J. Biol. Chem. 271: 27715-27722.
19. Honig, L. S., Chambliss, D. D., Bigio, E. H., Carroll, S. L., and Elliott, J. L. (2000). Glutamate transporter EAAT2 splice variants occur not only in ALS, but also in AD and controls. Neurology 55: 1082-1088.
20. Ikonomidou, C., Qin, Y., Labruyere, J., and Olney, J. W. (1996). Motor neuron degeneration induced by excitotoxin agonists has features in common with those seen in the SOD-1 transgenic mouse model of amyotrophic lateral sclerosis. J. Neuropathol. Exp. Neurol. 55: 211-224.
21. Ince, P., Stout, N., Shaw, P., Slade, J., Hunziker, W., Heizmann, C. W., and Baimbridge, K. G. (1993). Parvalbumin and calbindin D-28k in the human motor system and in motor neuron disease. Neuropathol. Appl. Neurobiol. 19: 291-299.
22. Jackson, M., Song, W., Liu, M. Y., Jin, L., Dykes-Hoberg, M., Lin, C. L. G., Bowers, W. J., Federoff, H. J., Sternweis, R. C., and Rothstein, J. D. (2001). Modulation of the neuronal glutamate transporter EAAT4 by two interacting proteins. Nature 410: 89-93.
23. Kanai, Y., and Hediger, M. A. (1992). Primary structure and functional characterization of a high-affinity glutamate transporter. Nature 260: 467-471.
24. Kugler, P., and Schmitt, A. (1999). Glutamate transporter EAAC1 is expressed in neurons and glial cells in the rat nervous system. Glia 27: 129-142.
25. Li, S., Mallory, M., Alford, M., Tanaka, S., and Masliah, E. (1997). Glutamate transporter alterations in Alzheimer disease are possibly associated with abnormal APP expression. J. Neuropathol. Exp. Neurol. 56: 901-911.
26. Lin, C. L. G., Bristol, L. A., Jin, L., Dykes-Hoberg, M., Crawford, T., Clawson, L., and Rothstein, J. D. (1998). Aberrant RNA processing in a neurodegenerative disease: The cause for aberrant EAAT2, a glutamate transporter, in amyotrophic lateral sclerosis. Neuron 20: 589-602.
27. Lin, C. L. G., Orlov, I., Ruggerio, A. M., Dykes-Hoberg, M., Lee, A., Jackson, M., and Rothstein, J. D. (2001). Modulation of the neuronal glutamate transporter EAAC1 by an interacting protein GTRAP3-18. Nature 410: 84-88.
28. Masliah, E., Alford, M., De Teresa, R., Mallory, M., and Hansen, L. (1996). Deficient glutamate transport is associated with neurode-generation in Alzheimer's disease. Ann. Neurol. 40: 759-766.
29. Meyer, T., Fromm, A., Münch, C., Stamm, S., Fray, A. E., Ince, P. G., Stamm, S., Grön, G., Ludolph, A. C., and Shaw, P. J. (1999). The RNA of the glutamate transporter EAAT2 is variably spliced in amyotrophic lateral sclerosis and normal individuals. J. Neurol. Sci. 170: 45-50.
30. Münch, C., Penndorf, A., Schwalenstocker, B., Troost, D., Ludolph, A. C., Ince, P., and Meyer, T. (2001). Impaired RNA splicing of 53-regulatory sequences of the astroglial glutamate transporter EAAT2 in human astrocytoma. J. Neurol. Neurosurg. Psychiatry 71: 675-678.
31. Nagai, M., Abe, K., Okamoto, K., and Itoyama, Y. (1998). Identification of alternative splicing forms of GLT-1 mRNA in the spinal cord of amyotrophic lateral sclerosis patients. Neurosci. Lett. 244: 165-168.
32. Perego, C., Vanoni, C., Bossi, M., Massari, S., Basudev, H., Longhi, R., and Pietrini, G. (2000). The GLT-1 and GLAST glutamate transporters are expressed on morphologically distinct astrocytes and regulated by neuronal activity in primary hippocampal cocultures. J. Neurochem. 75: 1076-1084.
33. Pines, G., Danbolt, N. C., Bjøras, M., Zhang, Y., Bendahan, A., Eide, L., Koepsell, H., Storm-Mathisen, J., Seeberg, E., and Kanner, B. I. (1992). Cloning and expression of a rat brain L-glutamate transporter. Nature 360: 464-467.
34. Rothstein, J. D., Jin, L., Dykes-Hoberg, M., and Kuncl, R. W. (1993). Chronic inhibition of glutamate uptake produces a model of slow neurotoxicity. Proc. Natl. Acad. Sci. USA 90: 6591-6595.
35. Rothstein, J. D., Martin, L., Levey, A. I., Dykes-Hoberg, M., Jin, L., Wu, D., Nash, N., and Kuncl, R. W. (1994). Localization of neuronal and glial glutamate transporters. Neuron 13: 713-725.
36. Rothstein, J. D., Martin, L. J., and Kuncl, R. W. (1992). Decreased glutamate transport by the brain and spinal cord in amyotrophic lateral sclerosis. N. Engl. J. Med. 326: 1464-1468.
37. Rothstein, J. D., Tsai, G., Kuncl, R. W., Clawson, L., Cornblath, D. R., Drachman, D. B., Pestronk, A., Stauch, B. L., and Coyle, J. T. (1990). Abnormal excitatory amino acid metabolism in amyotrophic lateral sclerosis. Ann. Neurol. 28: 18-25.
38. Rothstein, J. D., Van Kammen, M., Levey, A. I., Martin, L. J., and Kuncl, R. W. (1995). Selective loss of glial glutamate transporter GLT-1 in amyotrophic lateral sclerosis. Ann. Neurol. 38: 73-84.
39. +-dependent glutamate transporters by cyclic AMP analogs and neurons. Mol. Pharmacol. 53: 355-369.
40. Schmitt, A., Asan, E., Lesch, K. P., and Kugler, P. (2002). A splice variant of glutamate transporter GLT1/EAAT2 expressed in neurons: Cloning and localization in rat nervous system. Neuroscience 109: 45-61.
41. Shifman, M. I., and Stein, D. G. (1995). A reliable and sensitive methods for nonradioactive Northern blot analysis of nerve growth factor mRNA from brain tissues. J. Neurosci. Methods 59: 205-208.
42. +-dependent glutamate/aspartate transporter from rat brain. Proc. Natl. Acad. Sci. USA 89: 10955-10959.