The RNA of the glutamate transporter EAAT2 is variably spliced in amyotrophic lateral sclerosis and normal individuals

Journal of the Neurological Sciences

Volumn 170, Issue 1, 1999, Pages 45-50

  Meyer, Thomas ^a   Fromm, Andrea ^a   Münch, Christoph ^a   Schwalenstöcker, Birgit ^a   Fray, Anne E ^b   Ince, Paul G ^b   Stamm, Stefan ^c   Grön, Georg ^a   Ludolph, Albert C ^a   Shaw, Pamela J ^b  

^a UNIVERSITY OF ULM   (Germany)

^b NEWCASTLE UNIVERSITY   (United Kingdom)

^c MAX PLANCK INSTITUTE OF NEUROBIOLOGY   (Germany)

Author keywords

Amyotrophic lateral sclerosis; EAAT2; Excitatory amino acid transporter; Glutamate; RNA; Splicing

Indexed keywords

CARRIER PROTEIN; EXCITATORY AMINO ACID; GLUTAMIC ACID; RNA;

AMINO ACID TRANSPORT; AMYOTROPHIC LATERAL SCLEROSIS; ARTICLE; CONTROLLED STUDY; GENE AMPLIFICATION; GENE DELETION; HUMAN; HUMAN TISSUE; INTRON; MOTOR CORTEX; PRIORITY JOURNAL; PROTEIN DEPLETION; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA SPLICING;

AGED; AMYOTROPHIC LATERAL SCLEROSIS; CADAVER; EXCITATORY AMINO ACID TRANSPORTER 2; HUMANS; MOTOR CORTEX; RECEPTORS, NEUROTRANSMITTER; REFERENCE VALUES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA; RNA SPLICING; VARIATION (GENETICS);

EID: 0032716796     PISSN: 0022510X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-510X(99)00196-3     Document Type: Article

References (18)

1. Tandan R. Clinical features and differential diagnosis of classical motor neuron disease. Williams A.C. Motor neuron disease. 1994;1-27 Chapman and Hall Medical, London.
2. Rosen D.R., Siddique T., Patterson D., Figlewicz D.A., Sapp P., Hentati A., Donaldson D., Goto J., O'Regan J.P., Deng H.X., et al. Mutations in Cu/Zn superoxide dismutase gene are associated with familial amyotrophic lateral sclerosis. Nature. 362:1993;59-62.
3. Feldmeyer D., Kask K., Brusa R., Kornau H.C., Kolhekar R., Rozov A., Burnashev N., Jensen V., Hvalby O., Sprengel R., Seeburg P. Neurological dysfunctions in mice expressing different levels of the Q/R site-unedited AMPAR subunit GluR-B. Nat Neurosci. 2:1999;57-64.
4. Rothstein J.D., Martin L.J., Kuncl R.W. Decreased glutamate transport by the brain and spinal cord in amyotrophic lateral sclerosis. N Engl J Med. 326:1992;1464-1468.
5. Rothstein J.D., Van Kammen M., Levey A.I., Martin L.J., Kuncl R.W. Selective loss of glial glutamate transporter GLT-1 in amyotrophic lateral sclerosis. Ann Neurol. 38:1995;73-84.
6. Lin C.L., Bristol L.A., Jin L., Dykes-Hoberg M., Crawford T., Clawson L., Rothstein J.D. Aberrant RNA processing in a neurodegenerative disease: the cause for absent EAAT2, a glutamate transporter, in amyotrophic lateral sclerosis. Neuron. 20:1998;589-602.
7. Meyer T., Munch C., Knappenberger B., Liebau S., Volkel H., Ludolph A.C. Alternative splicing of the glutamate transporter EAAT2 (GLT-1). Neurosci Lett. 241:1998;68-70.
8. Meyer T., Munch C., Liebau S., Fromm A., Schwalenstöcker B., Völkel H., Ludolph A.C. Splicing of the glutamate transporter EAAT2: a candidate gene of amyotrophic lateral sclerosis. J Neurol Neurosurg Psychiatry. 65:1998;954.
9. Aoki M., Lin C.L., Rothstein J.D., Geller B.A., Hosler B.A., Munsat T.L., Horvitz H.R., Brown R.H. Jr. Mutations in the glutamate transporter EAAT2 gene do not cause abnormal EAAT2 transcripts in amyotrophic lateral sclerosis. Ann Neurol. 43:1998;645-653.
10. Meyer T., Ludolph A.C., Morkel M., Hagemeier C., Speer A. Genomic organization of the human excitatory amino acid transporter gene GLT-1. Neuroreport. 8:1997;775-777.
11. Brooks B.R. El Escorial World Federation of Neurology criteria for the diagnosis of amyotrophic lateral sclerosis. Subcommittee on Motor Neuron Diseases/Amyotrophic Lateral Sclerosis of the world Federation of Neurology Research Group on Neuromuscular Diseases and the El Escorial 'Clinical limits of amyotrophic lateral sclerosis' workshop contributors. J Neurol Sci. 124:(Suppl):1994;96-107.
12. Fray A.E., Ince P.G., Banner S.J., Milton I.D., Usher P.A., Cookson M.R., Shaw P.J. The expression of the glial glutamate transporter protein EAAT2 in motor neuron disease: an immunohistochemical study. Eur J Neurosci. 10:1998;2481-2489.
13. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate phenol chloroform extraction. Anal Biochem. 162:1987;156-159.
14. Meyer T., Speer A., Meyer B., Sitte W., Kuther G., Ludolph A.C. The glial glutamate transporter complementary DNA in patients with amyotrophic lateral sclerosis. Ann Neurol. 40:1996;456-459.
15. Arriza J.L., Fairmann W.A., Wadiche J.I., Murdoch G.H., Kavanaugh M.P., Amara S.G. Functional comparisons of three glutamate transporter subtypes cloned from human motor cortex. J Neurosci. 14:1994;5559-5569.
16. Stamm S., Zhang M.Q., Marr T.G., Helfman D.M. A sequence compilation and comparison of exons that are alternatively spliced in neurons. Nucl Acids Res. 22:1994;1515-1526.
17. Smith C.W., Patton J.G., Nadal-Ginard B. Alternative splicing in the control of gene expression. Annu Rev Genet. 23:1989;527-577.
18. Moore J.M., Query C.C., Sharp P.A. Splicing of precursors to mRNA by the spliceosome. Gesteland R.F., Atkins J.F. The RNA world. 1993;303-357 Cold Spring Harbour Laboratory Press, New York.