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Baron U., Gossen M., Bujard H. Tetracycline-controlled transcription in eukaryotes: novel transactivators with graded transactivation potential. Nucleic Acids Res. 25:1997;2723-2729.
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Exploring the sequence space for tetracycline-dependent transcriptional activators: Novel mutations yield expanded range and sensitivity
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Novel rtTAs were generated by mutagenesis of the original tTA. One of them (rtTA-M2) shows a 10-fold higher affinity for dox than the previous version. Thus, a lower concentration of dox is required to induce rtTA-M2 DNA binding, facilitating its use in vivo.
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Urlinger S., Baron U., Thellmann M., Hasan M.T., Bujard H., Hillen W. Exploring the sequence space for tetracycline-dependent transcriptional activators: novel mutations yield expanded range and sensitivity. Proc Natl Acad Sci USA. 97:2000;7963-7968. Novel rtTAs were generated by mutagenesis of the original tTA. One of them (rtTA-M2) shows a 10-fold higher affinity for dox than the previous version. Thus, a lower concentration of dox is required to induce rtTA-M2 DNA binding, facilitating its use in vivo.
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Urlinger, S.1
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Stringent control of gene expression in vivo by using novel doxycycline-dependent trans-activators
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This report describes a method to achieve long-term, inducible expression of a target gene in vivo by direct delivery of the tet system to skeletal muscle. The authors used the novel rtTA-M2 transcriptional activator combined with DNA electroinjection-mediated delivery to obtain regulatable expression of therapeutic levels of EPO in mouse.
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Lamartina S., Roscilli G., Rinaudo C.D., Sporeno E., Silvi L., Hillen W., Bujard H., Cortese R., Ciliberto G., Toniatti C. Stringent control of gene expression in vivo by using novel doxycycline-dependent trans-activators. Hum Gene Ther. 13:2002;199-210. This report describes a method to achieve long-term, inducible expression of a target gene in vivo by direct delivery of the tet system to skeletal muscle. The authors used the novel rtTA-M2 transcriptional activator combined with DNA electroinjection-mediated delivery to obtain regulatable expression of therapeutic levels of EPO in mouse.
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Lamartina, S.1
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7
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0033767759
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Streptogramin-based gene regulation systems for mammalian cells
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This report describes a novel inducible system conceptually based on the tet system, but using a different antibiotic/transcription factor pair. This new system can be combined with the tet system to independently regulate multiple genes.
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Fussenegger M., Morris R.P., Fux C., Rimann M., von Stockar B., Thompson C.J., Bailey J.E. Streptogramin-based gene regulation systems for mammalian cells. Nat Biotechnol. 18:2000;1203-1208. This report describes a novel inducible system conceptually based on the tet system, but using a different antibiotic/transcription factor pair. This new system can be combined with the tet system to independently regulate multiple genes.
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Fussenegger, M.1
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0035866183
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Streptogramin- and tetracycline-responsive dual regulated expression of p27(Kip1) sense and antisense enables positive and negative growth control of Chinese hamster ovary cells
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Fux C., Moser S., Schlatter S., Rimann M., Bailey J.E., Fussenegger M. Streptogramin- and tetracycline-responsive dual regulated expression of p27(Kip1) sense and antisense enables positive and negative growth control of Chinese hamster ovary cells. Nucleic Acids Res. 29:2001;E19.
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This report contains an interesting analysis of the parameters influencing the efficiency of the tet system in transgenic animals.
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Robertson A., Perea J., Tolmachova T., Thomas P.K., Huxley C. Effects of mouse strain, position of integration and tetracycline analogue on the tetracycline conditional system in transgenic mice. Gene. 282:2002;65-74. This report contains an interesting analysis of the parameters influencing the efficiency of the tet system in transgenic animals.
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Use of the tetracycline-controlled transcriptional silencer (tTS) to eliminate transgene leak in inducible overexpression transgenic mice
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The authors used tTS (a transcriptional silencer consisting of tetR fused to the KRAB domain from the Kid-1 protein) to suppress leaky expression of an inducible transgene in the absence of inducer. Lung-specific expression of tTS repressed an inducible IL-13 transgene by at least three orders of magnitude, and did not interfere with the ability to induce it.
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Zhu Z., Ma B., Homer R.J., Zheng T., Elias J.A. Use of the tetracycline-controlled transcriptional silencer (tTS) to eliminate transgene leak in inducible overexpression transgenic mice. J Biol Chem. 276:2001;25222-25229. The authors used tTS (a transcriptional silencer consisting of tetR fused to the KRAB domain from the Kid-1 protein) to suppress leaky expression of an inducible transgene in the absence of inducer. Lung-specific expression of tTS repressed an inducible IL-13 transgene by at least three orders of magnitude, and did not interfere with the ability to induce it.
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Aubel, D.8
Bailey, J.E.9
Fussenegger, M.10
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