The Tet-on system in transgenic mice: Inhibition of the mouse pdx-1 gene activity by antisense RNA expression in pancreatic β-cells

Journal of Molecular Medicine

Volumn 79, Issue 5-6, 2001, Pages 321-328

  Lottmann, Heike ^a   Vanselow, Jens ^b   Hessabi, Behnam ^a   Walther, Reinhard ^a  

^a UNIVERSITY OF GREIFSWALD   (Germany)

^b LEIBNIZ INSTITUTE FOR FARM ANIMAL BIOLOGY FBN   (Germany)

Author keywords

Antisense; Insulin; PDX 1; Tet system; Transgenic mice

Indexed keywords

COMPLEMENTARY RNA; DNA; GENE PRODUCT; GLUCAGON; GLUCOKINASE; GLUCOSE TRANSPORTER; GLUCOSE TRANSPORTER 2; INSULIN; TRANSCRIPTION FACTOR PDX 1; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CONTROLLED STUDY; DOWN REGULATION; GENE ACTIVITY; GENE EXPRESSION; GENETIC TRANSCRIPTION; GLUCOSE TOLERANCE; HOMEOBOX; IMMUNOFLUORESCENCE TEST; IMMUNOHISTOCHEMISTRY; MOUSE; NONHUMAN; PANCREAS ISLET BETA CELL; TRANSGENIC MOUSE;

AMYLOID; ANIMALS; DOWN-REGULATION; DOXYCYCLINE; FEMALE; GLUCAGON; GLUCOKINASE; GLUCOSE; GLUCOSE TOLERANCE TEST; GLUCOSE TRANSPORTER TYPE 2; HOMEODOMAIN PROTEINS; IMMUNOHISTOCHEMISTRY; INSULIN; ISLETS OF LANGERHANS; MALE; MICE; MICE, INBRED C57BL; MICE, INBRED DBA; MICE, TRANSGENIC; MONOSACCHARIDE TRANSPORT PROTEINS; ORGAN SPECIFICITY; PROMOTER REGIONS (GENETICS); RESPONSE ELEMENTS; RNA, ANTISENSE; RNA, MESSENGER; TETRACYCLINE; TRANS-ACTIVATORS;

EID: 0034940450     PISSN: 09462716     EISSN: None     Source Type: Journal    
DOI: 10.1007/s001090100229     Document Type: Article

References (29)

1. Tight control of gene expression in mammalian cells by tetracycline-responsive promoters
2. Transcriptional activation by tetracyclines in mammalian cells
3. A tetracycline controlled activation/repression system with increased potential for gene transfer into mammalian cells
4. Exploring the sequence space for tetracycline-dependent transcriptional activators: Novel mutations yield expanded range and sensitivity
5. Insulin-promoter-factor-1 is required for pancreas development in mice
6. PDX-1 is required for pancreatic outgrowth and differentiation of the rostral duodenum
7. βb-cell-specific inactivation of the mouse Ipf1/Pdx1 gene results in loss of the βb-cell phenotype and maturity onset diabetes
8. IDX-1: A new homeodomain transcription factor expressed in rat pancreatic islets and duodenum that transactivates the somatostatin gene
9. Expression of STF-1, a putative insulin gene transcription factor, in β-cells of pancreas, duodenal epithelium and pancreatic exokrine and endocrine progenitors during ontogeny
10. IPF-1, a homeodomain-containing transactivator of the insulin gene
11. Involvement of the homeodomain-containing transcription factor PDX-1 in islet amyloid polypeptide gene transcription
12. Transcriptional activation of the GLUT2 gene by the IPF-1/STF-1/IDX-1 homeobox factor
13. The human glucokinase gene β-cell-type promoter: An essential role of insulin promoter factor 1/ PDX-1 in its activation in HIT-T15 cells
14. Temporal control of gene expression in transgenic mice by a tetracycline-responsive promoter
15. Doxycycline-mediated quantitative and tissue-specific control of gene expression in transgenic mice
16. Induction of insulin and islet amyloid polypeptide production in pancreatic islet glucagonoma cells by insulin promoter factor 1
17. PDX-1 induces insulin and glucokinase gene expression in αTC1 clone 6 cells in the presence of betacellulin
18. Functional regions of the homeodomain protein IDX-1 required for transactivation of the rat somatostatin gene
19. Reduced β-cell glucose transporter in new onset diabetic BB rats
20. Expression of Glut-2 antisense RNA in β cells of transgenic mice leads to diabetes
21. Early diabetes and abnormal postnatal pancreatic islet development in mice lacking Glut-2
22. Tissue-specific expression of glucokinase: Identification of the gene product in liver and pancreatic islets
23. Signal recognition by pancreatic β-cells
24. Pancreatic islet glucose metabolism and regulation of insulin secretion
25. Banting Lecture 1995. A lesson in metabolic regulation inspired by the glucokinase glucose sensor paradigm
26. Human glucokinase gene: Isolation and characterization and identification of two missense mutations linked to early-onset noninsulin-dependent (type 2) diabetes mellitus
27. Primary pancreatic beta-cell secretory defect caused by mutations in glucokinase gene in kindreds of maturity onset diabetes of the young
28. Effects of alternate RNA splicing on glucokinase isoform activities in the pancreatic islet, liver, and pituitary