Cloning, nucleotide sequence and expression of a hydantoinase and carbamoylase gene from Arthrobacter aurescens DSM 3745 in Escherichia coli and comparison with the corresponding genes from Arthrobacter aurescens DSM 3747

Applied Microbiology and Biotechnology

Volumn 55, Issue 6, 2001, Pages 750-757

  Wiese, Anja ^a   Wilms, Burkhard ^a   Syldatk, Christoph ^a   Mattes, Ralf ^a   Altenbuchner, Josef ^a  

^a UNIVERSITY OF STUTTGART   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CARBAMOYLASE; CHITIN; DIHYDROPYRIMIDINASE; ENZYME; INTEIN; MALTOSE BINDING PROTEIN; RHAMNOSE; UNCLASSIFIED DRUG;

BACTERIUM;

AMINO ACID SEQUENCE; ARTHROBACTER; ARTHROBACTER AURESCENS; ARTICLE; BACTERIUM; BASE PAIRING; DNA SEQUENCE; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE EXPRESSION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PLASMID; PROTEIN SYNTHESIS; STEREOSPECIFICITY; THERMOSTABILITY;

AMIDOHYDROLASES; ARTHROBACTER; ATP-BINDING CASSETTE TRANSPORTERS; CARRIER PROTEINS; CHITIN; CLONING, MOLECULAR; ENZYME STABILITY; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENES, BACTERIAL; HYDANTOINS; HYDROGEN-ION CONCENTRATION; MONOSACCHARIDE TRANSPORT PROTEINS; PERIPLASMIC BINDING PROTEINS; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS; SEQUENCE ANALYSIS, DNA; STEREOISOMERISM; SUBSTRATE SPECIFICITY; TEMPERATURE;

EID: 0034911505     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002530000574     Document Type: Article

References (23)

1. A new λ RES vector with a built-in Tn1721-encoded excision system
2. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding
3. Expression of correctly folded proteins in Escherichia coli
4. In vitro binding of the Salmonella dublin virulence plasmid regulatory protein SpvR to the promoter regions of spvA and spvR
5. Screening method for microorganisms producing L-amino acids from D,L-monosubstituted hydantoins
6. An evolutionary treasure: Unification of a broad set of amidohydrolases related to urease
7. Cleavage of structure proteins during the assembly of the head of bacteriophage T4
8. The pIC plasmid and phage vectors with versatile cloning sites for recombinant selection by insertional inactivation
9. Substrate-dependent enantioselectivity of a novel hydantoinase from Arthrobacter aurescens DSM 3745: Purification and characterisation as a new member of cyclic amidases
10. Molecular evolution of hydantoinases
11. The hydantoin amidohydrolase from Arthrobacter aurescens DSM 3745 is a zinc metalloenzyme
12. Catalytic and structural function of zinc for the hydantoinase from Arthrobacter au-rescens DSM 3745
13. Diversity and versatility of microbial hydantoin-transforming enzymes
14. Purification of recombinant hydantoinase and L-N-carbamoylase from Arthrobacter aurescens expressed in Escherichia coli: Comparison of wildtype and genetically modified proteins
15. Biocatalytic production of amino acids and derivatives
16. Microbial hydantoinases - Industrial enzymes from the origin of life?
17. Nucleotide sequence and role in DNA amplification of the direct repeats composing the amplifiable element AUD1 of Streptomyces lividans 66
18. Cloning and sequencing of the genes involved in the conversion of 5-substituted hydantoins to the corresponding L-amino acid from the native plasmid of Pseudomonas sp. NS671
19. Cloning, nucleotide sequence and expression of a new L-N-carbamoylase gene from Arthrobacter aurescens DSM 3747 in E. coli
20. Hydantoin racemase from Arthrobacter aurescens DSM 3747: Heterologous expression, purification and characterization
21. Improved M13 phage cloning vectors and host strains: Nucleotide sequences of the M13mp18 and pUC vectors