Directed evolution of a novel N-carbamylase/D-hydantoinase fusion enzyme for functional expression with enhanced stability

Biotechnology and Bioengineering

Volumn 68, Issue 2, 2000, Pages 211-217

  Kim, Geun Joong ^a   Cheon, Young Hoon ^a   Kim, Hak Sung ^a  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

Author keywords

Bifunctional enzyme; D hydantoinase; Directed evolution; DNA shuffling; Linear fusion; N carbamylase

Indexed keywords

AMINO ACIDS; BIOCATALYSTS; BIOSYNTHESIS; BIOTECHNOLOGY; DNA; ESCHERICHIA COLI; POLYPEPTIDES;

CARBOMYLASE; DNA SHUFFLING; LINEAR FUSION; MOLECULAR BIOLOGY; PROTEOLYSIS;

ENZYMES;

DIHYDROPYRIMIDINASE; HYBRID PROTEIN; POLYPEPTIDE;

AMINO ACID METABOLISM; ARTICLE; CATALYST; ENZYME ACTIVITY; ENZYME SPECIFICITY; ENZYME STABILITY; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN EXPRESSION;

AMIDOHYDROLASES; AMINO ACIDS; BACILLUS STEAROTHERMOPHILUS; CLONING, MOLECULAR; DIRECTED MOLECULAR EVOLUTION; ENZYME STABILITY; RECOMBINANT FUSION PROTEINS; RHIZOBIUM;

ESCHERICHIA COLI;

EID: 0000259981     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(20000420)68:2<211::AID-BIT10>3.0.CO;2-P     Document Type: Article

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