Stabilization of NAD-dependent formate dehydrogenase from Candida boidinii by site-directed mutagenesis of cysteine residues

European Journal of Biochemistry

Volumn 267, Issue 5, 2000, Pages 1280-1289

  Slusarczyk, Heike ^a   Felber, Stephan ^b   Kula, Maria Regina ^b   Pohl, Martina ^b  

^a HEINRICH HEINE UNIVERSITY   (Germany)

^b NONE

Author keywords

Candida boidinii; Formate dehydrogenase; NAD dependent; Site directed mutagenesis; Stabilization

Indexed keywords

COPPER ION; CYSTEINE; FORMATE DEHYDROGENASE; NICOTINAMIDE ADENINE DINUCLEOTIDE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; CANDIDA BOIDINII; ENZYME STABILITY; ENZYME STRUCTURE; GENE EXPRESSION; GENE INACTIVATION; GENE MUTATION; NONHUMAN; NUCLEOTIDE SEQUENCE; OXIDATIVE STRESS; PRIORITY JOURNAL; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS; TEMPERATURE DEPENDENCE;

AMINO ACID SEQUENCE; BASE SEQUENCE; CATALYSIS; CLONING, MOLECULAR; CYSTEINE; DNA PRIMERS; DNA, FUNGAL; ENZYME STABILITY; FORMATE DEHYDROGENASES; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; NAD; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID;

CANDIDA BOIDINII; ESCHERICHIA COLI; PSEUDOMONAS;

EID: 0034011107     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.2000.01123.x     Document Type: Article

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