Asn249Tyr substitution at the coenzyme binding domain activates Sulfolobus solfataricus alcohol dehydrogenase and increases its thermal stability

Biochemistry

Volumn 38, Issue 10, 1999, Pages 3043-3054

  Giordano, A ^a   Cannio, R ^a   La Cara, F ^a   Bartolucci, S ^b   Rossi, M ^a,b   Raia, C A ^a  

^a CNR   (Italy)

^b UNIVERSITY OF NAPLES FEDERICO II   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

ALCOHOL DEHYDROGENASE; BENZALDEHYDE; BENZYL ALCOHOL; CYCLOHEXANOL; PROPANOL; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE DEHYDROGENASE (UBIQUINONE); TYROSINE; ZINC;

ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME BINDING; ENZYME DENATURATION; ENZYME INACTIVATION; ENZYME ISOLATION; ENZYME KINETICS; ENZYME MECHANISM; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYME SUBSTRATE; NONHUMAN; OXIDATION REDUCTION REACTION; PRIORITY JOURNAL;

ALCOHOL DEHYDROGENASE; AMIDES; ASPARAGINE; BINDING SITES; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CIRCULAR DICHROISM; COENZYMES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME ACTIVATION; ENZYME STABILITY; ESCHERICHIA COLI; GUANIDINE; HEAT; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; KINETICS; PEPTIDE FRAGMENTS; PROTEIN DENATURATION; RECOMBINANT PROTEINS; SPECTROMETRY, FLUORESCENCE; SULFOLOBUS; TYROSINE;

ARCHAEA; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; SULFOLOBUS SOLFATARICUS;

EID: 0033537704     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi982326e     Document Type: Article

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