Mutagenesis and modelling of the α1b-adrenergic receptor highlight the role of the helix 3/helix 6 interface in receptor activation

Molecular Pharmacology

Volumn 61, Issue 5, 2002, Pages 1025-1032

  Greasley, Peter J ^a,b   Fanelli, Francesca ^a,b   Rossier, Olivier ^a,b   Abuin, Liliane ^a,b   Cotecchia, Susanna ^a,b  

^a UNIVERSITY OF LAUSANNE   (Switzerland)

^b UNIVERSITY OF MODENA AND REGGIO EMILIA   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA 1B ADRENERGIC RECEPTOR; ARGININE; GLUTAMIC ACID; RHODOPSIN;

ANIMAL CELL; ARTICLE; COMPUTER SIMULATION; CRYSTAL STRUCTURE; GENE SEQUENCE; GENETIC CONSERVATION; MUTAGENESIS; MUTATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN MOTIF; RECEPTOR UPREGULATION;

AMINO ACID SUBSTITUTION; ANIMALS; CERCOPITHECUS AETHIOPS; COS CELLS; CRICETINAE; MODELS, MOLECULAR; MUTAGENESIS; PROTEIN STRUCTURE, SECONDARY; RECEPTORS, ADRENERGIC, ALPHA-1; TRANSFECTION;

EID: 0036239236     PISSN: 0026895X     EISSN: None     Source Type: Journal    
DOI: 10.1124/mol.61.5.1025     Document Type: Article

References (25)

1. Activation of the β2-adrenergic receptor involves disruption of an ionic lock between the cytoplasmic ends of transmembrane segments 3 and 6
2. Integrated methods for the construction of three-dimensional models and computational probing of structure-function relations in G protein-coupled receptors
3. C5a receptor activation. Genetic identification of critical residues in four transmembrane helices
4. q/11 by the M3 muscarinic acetylcholine receptor
5. 1B-adrenergic receptor signal-inactivating point mutation
6. Phe310 in transmembrane VI of the α1B-adrenergic receptor is a key switch residue involved in activation and catecholamine ring aromatic bonding
7. 1-adrenergic receptor determine the selectivity of coupling to phosphatidylinositol hydrolysis
8. Backbone-dependent rotamer library for proteins. Application to side-chain prediction
9. Ab initio modeling and molecular dynamics simulation of the alpha1b-adrenergic receptor activation
10. Theoretical study on the electrostatically driven step of receptor-G protein recognition
11. Requirement of rigid-body motion of transmembrane helices for light activation of rhodopsin
12. Uncovering molecular mechanisms involved in activation of G protein-coupled receptors
13. Mutational and computational analysis of the α1b-adrenergic receptor: Involvement of basic and hydrophobic residues in receptor activation and G protein coupling
14. Agonist-induced conformational changes at the cytoplasmic side of transmembrane segment 6 in the β2 adrenergic receptor mapped by site-selective fluorescent labeling
15. 1B-adrenergic receptor by all amino acid substitutions at a single site
16. Structure and function in rhodopsin: Rhodopsin mutants with a neutral amino acid at E134 have a partially activated conformation in the dark state
17. Crystal structure of rhodopsin: A G protein-coupled receptor
18. Comparative protein modelling by satisfaction of spatial restraints
19. 2-adrenergic receptor. Extending the ternary complex model
20. 1b-adrenergic receptor: Effects on receptor isomerization and activation
21. Constitutively active mutants of the alpha1B-adrenergic receptor: Role of highly conserved polar amino acids in receptor activation
22. The activation process of the alpha1B-adrenergic receptor: Potential role of protonation and hydrophobicity of a highly conserved aspartate
23. Rhodopsin activation blocked by metal-ion-binding sites linking transmembrane helices C and F
24. A constitutively activating mutation of the luteinizing hormone receptor in familial male precocious puberty
25. A new constitutively activating point mutation in the luteinizing hormone choriogonadotropin receptor gene in cases of male-limited precocious puberty