Spt5 cooperates with human immunodeficiency virus type 1 Tat by preventing premature RNA release at terminator sequences

Molecular and Cellular Biology

Volumn 22, Issue 4, 2002, Pages 1079-1093

  Bourgeois, Cyril F ^a   Kim, Young Kyeung ^a   Churcher, Mark J ^a   West, Michelle J ^a   Karn, Jonathan ^a  

^a MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CYCLINE; ELONGATION FACTOR; ELONGATION FACTOR NUSG; PROTEIN KINASE; RNA POLYMERASE II; TAT ACTIVATED KINASE; TRANSACTIVATOR PROTEIN; TRANSCRIPTION ELONGATION FACTOR SPT5; UNCLASSIFIED DRUG; VIRUS RNA;

ARTICLE; CARBOXY TERMINAL SEQUENCE; COMPLEX FORMATION; CONTROLLED STUDY; DISSOCIATION; DNA SEQUENCE; DNA TEMPLATE; ENZYME ACTIVATION; ENZYME STABILITY; ENZYME SUBUNIT; ESCHERICHIA COLI; HUMAN IMMUNODEFICIENCY VIRUS 1; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN ISOLATION; PROTEIN PHOSPHORYLATION; PROTEIN RNA BINDING; REGULATOR GENE; RNA SEQUENCE; RNA TRANSLATION; SEQUENCE HOMOLOGY; TRANSACTIVATION; TRANSCRIPTION INITIATION;

CYCLIN-DEPENDENT KINASE 9; CYCLIN-DEPENDENT KINASES; DNA-DIRECTED RNA POLYMERASES; GENE PRODUCTS, TAT; HELA CELLS; HIV LONG TERMINAL REPEAT; HIV-1; HUMANS; MACROMOLECULAR SUBSTANCES; MODELS, GENETIC; NF-KAPPA B; NUCLEAR PROTEINS; POSITIVE TRANSCRIPTIONAL ELONGATION FACTOR B; PROTEIN-SERINE-THREONINE KINASES; RECOMBINANT PROTEINS; REPRESSOR PROTEINS; RNA, MESSENGER; TRANSCRIPTION FACTOR RELA; TRANSCRIPTION FACTORS; TRANSCRIPTION, GENETIC;

ESCHERICHIA COLI; HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1; RNA VIRUSES;

EID: 0036150085     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.22.4.1079-1093.2002     Document Type: Article

References (69)

1. Production of acquired immunodeficiency syndrome-associated retrovirus in human and non-human cells transfected with an infectious molecular clone
2. High-resolution localization of Drosophila Spt5 and Spt6 at heat shock genes in vivo: Roles in promoter proximal pausing and transcription elongation
3. Pausing by bacterial RNA polymerase is mediated by mechanistically distinct classes of signals
4. Transcription elongation through DNA arrest sites: A multistep process involving both RNA polymerase II subunit RPB9 and TFIIS
5. Tat trans-activates the human immunodeficiency virus through a nascent RNA target
6. Recruitment of a protein complex containing Tat and cyclin T1 to TAR governs the species specificity of HIV-1 Tat
7. Distinct roles for the helicases of TFIIH in transcript initiation and promoter escape
8. Specific interaction of Tat with the human but not rodent pTEFb complex mediates the species-specific Tat activation of HIV-1 transcription
9. Tat activates human immunodeficiency virus type I transcriptional elongation independent of TFIIH kinase
10. Purified yeast RNA polymerase II reads through intrinsic blocks to elongation in response to the yeast TFIIS analogue, p37
11. The RNA element encoded by the trans-activation-responsive region of human immunodeficiency virus type 1 is functional when displaced downstream of the start of transcription
12. Human immunodeficiency virus 1 Tat protein binds trans-activation-responsive region (TAR) RNA in vitro
13. Interactions between human cyclin T, Tat, and the transactivation response element (TAR) are disrupted by a cysteine to tyrosine substitution found in mouse cyclin
14. HIV-1 Tat: Coping with negative elongation factors
15. CDK9 autophosphorylation regulates high-affinity binding of the human immunodeficiency virus type 1 Tat-P-TEFb complex to TAR RNA
16. The interaction between HIV-1 Tat and human cyclin T1 requires zinc and a critical cysteine residue that is not conserved in the murine CycT1 protein
17. Human immunodeficiency virus type 1 trans-activator protein Tat, stimulates transcriptional read-through of distal terminator sequences in vitro
18. Transcriptional anti-termination
19. Evidence that Spt4, Spt5, and Spt6 control transcription elongation by RNA polymerase II in Saccharomyces cerevisiae
20. Lentivirus Tat proteins specifically associate with a cellular protein kinase, TAK, that hyperphosphorylates the carboxyl-terminal domain of the large subunit of RNA polymerase II: Candidate for a Tat cofactor
21. Direct evidence that HIV-1 Tat activates the Tat-associated kinase (TAK) during transcriptional elongation
22. Domains in the SPT5 protein that modulate its transcriptional regulatory properties
23. Cyclin T domains involved in complex formation with Tat and TAR RNA are critical for Tat-activation
24. Anti-termination of transcription within the long terminal repeat of HIV-1 by Tat gene product
25. Spt5 and Spt6 are associated with active transcription and have characteristics of general elongation factors in D. melanogaster
26. Tackling Tat
27. HIV-1 Tat acts as a processivity factor in vitro in conjunction with cellular elongation factors
28. Transfer of Tat and release of TAR RNA during the activation of the human immunodeficiency virus type-1 transcription elongation complex
29. Human immunodeficiency virus type-1 Tat is an integral component of the activated transcription-elongation complex
30. Analysis of signals for transcription termination by purified RNA polymerase II
31. Positive transcription elongation factor b phosphorylates hSPT5 and RNA polymerase II carboxyl-terminal domain independently of cyclin-dependent kinase-activating kinase
32. TFIIS enhances transcriptional elongation through an artificial arrest site in vivo
33. Promoter-proximal stalling results from the inability to recruit transcription factor IIH to the transcription complex and is a regulated event
34. Role of the human and murine cyclin T proteins in regulating HIV-1 Tat-activation
35. HIV-1 Tat protein increases transcriptional initiation and stabilizes elongation
36. Differential transcriptional activation in vitro by NF-κB/Rel proteins
37. Human immunodeficiency virus type 1 Tat-dependent activation of an arrested RNA polymerase II elongation complex
38. p-TEFb kinase is required for HIV Tat transcriptional activation in vivo and in vitro
39. HIV-1 Tat protein trans-activates transcription in vitro
40. HIV-1 Tat protein promotes formation of more-processive elongation complexes
41. Roles of RNA: DNA hybrid stability, RNA structure, and active site conformation in pausing by human RNA polymerase II
42. Transcriptional pausing at +62 of the HIV-1 nascent RNA modulates formation of the TAR RNA structure
43. Enhanced processivity of RNA polymerase II triggered by Tat-induced phosphorylation of its carboxy-terminal domain
44. A novel LBP-1-mediated restriction of HIV-1 transcription at the level of elongation in vitro
45. Regulation of Rho-dependent transcription termination by NusG is specific to the Escherichia coli elongation complex
46. Tat-associated kinase (P-TEF-b): A component of transcription preinitiation and elongation complexes
47. DSIF and NELF interact with RNA polymerase 11 elongation complex and HIV-1 Tat stimulates pTEFb-mediated phosphorylation of RNA polymerase II and DSIF during transcription elongation
48. P-TEFb, a cyclin-dependent kinase controlling elongation by RNA polymerase II
49. A highly purified RNA polymerase II elongation control system
50. The human immunodeficiency virus long terminal repeat includes a specialised initiator element which is required for Tat-responsive transcription
51. Structure, sequence and position of the stem-loop in TAR determine transcriptional elongation by Tat through the HIV-1 long terminal repeat
52. Location of the trans-acting region on the genome of human T-cell lymphotropic virus type III
53. Transcriptional trans-activation by human immunodeficiency virus type 1 Tat requires specific coactivators that are not basal factors
54. Tat transactivation: A model for the regulation of eukaryotic transcriptional elongation
55. Reconstitution of the transcription factor TFIIH: Assignment of functions for the three subunits XPB, XPD, and cdk7
56. An integrated model of the transcription complex in elongation, termination and editing
57. FACT relieves DSIF/NELF-mediated inhibition of transcriptional elongation and reveals functional differences between p-TEFb and TFIIH
58. Evidence that pTEFb alleviates the negative effect of DSIF on RNA polymerase II-dependent transcription in vitro
59. DSIF, a novel transcription elongation factor that regulates RNA polymerase II processivity, is composed of human Spt4 and Spt5 homologs
60. A novel cdk9-associated c-type cyclin interacts directly with HIV-1 Tat and mediates its high-affinity, loop specific binding to TAR RNA
61. Role of the human homolog of the yeast transcription factor SPT5 in HIV-1 Tat-activation
62. NELF, a multisubunit complex containing RD, cooperates with DSIF to repress RNA polymerase II elongation
63. Structure and function of the human transcription elongation factor DSIF
64. Recycling of the general transcription factors during RNA polymerase II transcription
65. Tat modifies the activity of CDK9 to phosphorylate serine 5 of the RNA polymerase II carboxyl-terminal domain during human immunodeficiency virus type 1 transcription
66. Transcription elongation factor p-TEFb mediates Tat activation of HIV-1 transcription at multiple stages
67. Novel mechanism and factor for regulation by HIV-1 Tat
68. Tat-SF1: Cofactor for stimulation of transcriptional elongation by HIV-1 Tat
69. Transcription elongation factor p-TEFb is required for HIV-1 Tat trans-activation in vitro