Description of a 96-well plate assay to measure cytochrome P4503A inhibition in human liver microsomes using a selective fluorescent probe

Analytical Biochemistry

Volumn 276, Issue 2, 1999, Pages 215-226

  Chauret, Nathalie ^a   Tremblay, Nathalie ^a   Lackman, Rebecca L ^a   Gauthier, Jacques Yves ^a   Silva, José M ^a   Marois, Jean ^a   Yergey, James A ^a   Nicoll Griffith, Deborah A ^a  

^a MERCK FROSST CENTRE FOR THERAPEUTIC RESEARCH   (Canada)

Author keywords

Cytochrome P450 inhibition; Fluorescent probe; Human liver microsomes

Indexed keywords

BIOMOLECULES; CELL CULTURE; FLUORESCENCE SPECTROSCOPY; LIVER; METABOLISM; METABOLITES; PEPTIDES; PROBES;

CHEMICAL INHIBITORS; CYTOCHROME P450; FLUORESCENT COMPOUNDS; FLUORESCENT PROBES; GLUTATHIONE REDUCTASE; HUMAN LIVER MICROSOMES; HYDROXY METABOLITES; POTENTIAL INHIBITORS;

FLUORESCENCE;

CYTOCHROME P450 3A; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE;

ARTICLE; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME INHIBITION; FLUORESCENCE ANALYSIS; HUMAN; HUMAN TISSUE; IMMUNOASSAY; LIVER MICROSOME; PRIORITY JOURNAL; QUANTITATIVE ASSAY;

STAPHYLOCOCCUS PHAGE 3A;

EID: 0033572466     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.1999.4348     Document Type: Article

References (22)

1. Spatzenegger M., Jaeger W. Clinical importance of hepatic cytochrome P450 in drug metabolism. Drug Metab. Rev. 27:1995;397-417.
2. Parkinson A. An overview of current cytochrome P450 technology for assessing the safety and efficacy of new materials. Toxicol. Pathol. 24:1996;48-57.
3. Crespi C. L., Miller V. P., Penman B. W. Microtiter plate assays for inhibition of human, drug-metabolizing cytochromes P450. Anal. Biochem. 248:1997;188-190.
4. Palamanda J. R., Favreau L., Lin C. C., Nomeir A. A. Validation of a rapid microtiter plate assay to conduct cytochrome P450 2D6 enzyme inhibition studies. Drug Discovery Today. 3:1998;466-470.
5. Shimada T., Yamazaki H., Mimura M., Inui Y., Guengerich F. P. Interindividual variations in human liver cytochrome P-450 enzymes involved in the oxidation of drugs, carcinogens and toxic chemicals: studies with liver microsomes of 30 Japanese and 30 Caucasians. J. Pharmacol. Exp. Ther. 270:1994;414-423.
6. Waxman D. J., Attisano C., Guengerich F. P., Lapenson D. P. Human liver microsomal steroid metabolism: Identification of the major microsomal steroid hormone 6 beta-hydroxylase cytochrome P-450 enzyme. Arch. Biochem. Biophys. 263:1988;424-436.
7. Waxman D. J., Lapenson D. P., Aoyama T., Gelboin H. V., Gonzalez F. J., Korzekwa K. Steroid hormone hydroxylase specificities of eleven cDNA-expressed human cytochrome P450s. Arch. Biochem. Biophys. 290:1991;160-166.
8. Ohmori S., Nakasa H., Asanome K., Kurose Y., Ishii I., Hosokawa M., Kitada M. Differential catalytic properties in metabolism of endogenous and exogenous substrates among CYP3A enzymes expressed in COS-7 cells. Biochim. Biophys. Acta. 1380:1998;297-304.
9. Newton D. J., Wang R. W., Lu A. Y. H. Cytochrome P450 inhibitors: Evaluation of specificities in the in vitro metabolism of therapeutic agents by human liver microsomes. Drug Metab. Dispos. 23:1995;154-158.
10. Crespi C. L., Penman B. W. Li A. Advances in Pharmacology, Drug-drug Interactions, Scientific and Regulatory Perspectives. 1997;171-188 Academic Press, San Diego.
11. Crespi C. L., Miller V. P., Ackermann J. M., Stresser D. M., Busby W. F. Novel high throughput fluorescent P450 assays. Toxicologist. 48:1999.
12. Mei, Q, Tang, C, Assang, C, Slaughter, D, Rodrigues, A. D, Baillie, T. A, Rushmore, T. H, and, Shou, M. 1999, Role of a potent inhibitory monoclonal antibody to cytochrome P4503A in assessment of human drug metabolism. Submitted for publication.
13. Lu A. Y., Levin W. Partial purification of cytochrome P-450 and cytochrome P-448 from rat microsomes. Biochem. Biophys. Res. Commun. 46:1972;1334-1339.
14. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:1951;265-275.
15. Chauret N., Gauthier A., Nicoll-Griffith D. A. Effect of common organic solvents on in vitro cytochrome P450-mediated metabolic activities in human liver microsomes. Drug Metab. Dispos. 26:1998;1-4.
16. Sonderfan A. J., Arlotto M. P., Dutton D. R., McMillen S. K., Parkinson A. Regulation of testosterone hydroxylation by rat liver microsomal cytochrome P-450. Arch. Biochem. Biophys. 255:1987;27-41.
17. Moré, J, Garbow, B, and, Hillstrom, K. 1980, User Guide for MINIPACK-1. Argonne National Laboratory Report ANL-80-75, Argonne, IL.
18. Chauret N., Gauthier A., Martin J., Nicoll-Griffith D. A. In vitro comparison of cytochrome P450-mediated metabolic activities in human, dog, cat and horse. Drug Metab. Dispos. 25:1997;1130-1136.
19. Nicoll-Griffith, D. A, Chauret, N, Day, S, Leblanc, Y, Roy, P, Silva, J, Yergey, J. A, Dixit, R, and, Patrick, D. 1999, Prediction of metabolic autoinduction using rat hepatocytes in culture, presented at the International Society for the Study of Xenobiotics, 9th North American Meeting (ISSX) and American Cancer Society Division of Chemical Toxicology (ACS/DCTJoint Meeting), October 1999. Nashville, TN.
20. Smith D. A., Jones B. C., Walker D. K. Design of drugs involving the concepts and theories of drug metabolism and pharmacokinetics. Med. Res. Rev. 16:1996;243-266.
21. Greenlee W. F., Poland A. An improved assay of 7-ethoxycoumarin O-deethylase activity: Induction of hepatic enzyme activity in C57BL/6J and DBA/2J mice by phenobarbital, 3-methylcholanthrene and 2,3,7,8-tetrachlorodibenzo-p-dioxin. J. Pharmacol. Exp. Ther. 205:1978;596-605.
22. Pichard L., Fabre I., Fabre G., Domergue J., Saint Aubert B., Mourad G., Maurel P. Cyclosporin A drug interactions: Screening for inducers and inhibitors of cytochrome P-450 (cyclosporin A oxidase) in primary cultures of human hepatocytes and in human liver microsomes. Drug Metab. Dispos. 18:1990;595-606.