Production and purification of recombinant hirudin expressed in the methylotrophic yeast Pichia pastoris

Protein Expression and Purification

Volumn 8, Issue 4, 1996, Pages 476-482

  Rosenfeld, Stuart A ^a   Nadeau, Daniel ^a   Tirado, Joel ^a   Hollis, Gregory F ^a   Knabb, Robert M ^a   Steve, Jia ^a  

^a DUPONT COMPANY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0030475733     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1996.0127     Document Type: Article

References (25)

1. 1. Dodt, J., Müller, H. P., Seemüller, U., and Chang, J. Y. (1984) The complete amino acid sequence of hirudin, a thrombin specific inhibitor. FEBS Lett. 165, 180-184.
2. 2. Seemmuller, U., Dodt, J., Fink, E., and Fritz, H. (1986) Proteinase inhibitors of the leech Hirudo medicinialis (hirudins, bdellins, eglins), in "Proteases" (Barrett, A. J., Salvesen, G., Eds.), pp. 337-359, Elsevier, Amsterdam.
3. 3. Markwardt, F. (1989) Development of hirudin as an antithrombotic agent. Sem. Thromb. Hemostasis 15, 269-282.
4. 4. Lehman, E. D., Joyce, J. F., Bailey, J., Markus, H. Z., Schultz, L. D., Dunwiddie, C. T., Jacobson, M. A., and Miller, W. J. (1993) Expression, purification and characterization of multigram amounts of a recombinant hybrid HV1-HV2 hirudin variant expressed in Saccharomyces cerevisiae. Protein Express. Purif. 4, 247-255.
5. 5. Scorer, C. A., Clare, J. J., McCombie, W. R., Romanos, M. A., and Sreekrishna, K. (1994) Rapid selection using G418 of high copy number transformants of Pichia pastoris for high-level foreign gene expression. Bio / Technology 12, 181-184.
6. 6. Brake, A. J., Merryweather, J. P., Coit, D. G., Herberlein, U. A., Masiarz, F. R., Mullenbach, G. T., Urdea, M. S., Valenzuela, P., and Barr, P. J. (1984) α-Factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 81, 4642-4646.
7. 7. Cregg, J. M., Barringer, K. J., and Hessler, A. Y. (1985) Pichia pastoris as a host system for transformations. Mol. Cell Biol. 5, 3376-3385.
8. 8. Clare, J. J., Rayment, F. B., Ballantine, S. P., Sreekrishna, K., and Romanes, M. A. (1991) High-level expression of tetanus toxin fragment c in Pichia pastoris strains containing multiple tandem integrations of the gene. Bio / Technology 9, 455-460.
9. 9. Kettner, C., Mersinger, L., and Knabb, R. (1990) The selective inhibition of thrombin by peptides of boroarginine. J. Biol. Chem. 265, 18289-18297.
10. 10. Romanes, M. (1995) Advances in the use of Pichia pastoris for high-level expression. Curr. Op. Biotech. 6, 527-533.
11. 11. Clare, J. J., Romanes, M. A., Rayment, F. B., Rowedder, J. E., Smith, M. A., Payne, M. M., Sreekrishna, K., and Henwood, C. A. (1991) Production of mouse epidermal growth factor in yeast: High level secretion using Pichia pastoris strains containing multiple gene copies. Gene 105, 205-212.
12. 12. Brierley, R. A., Davis, G. R., and Holtz, G. C. (1994) Production of insulin-like growth factor-1 in methylotrophic yeast cells. United States Patent 5,324,639.
13. 13. Cregg, J. M., Vedvick, T. S., and Raschke, W. C. (1993) Recent advances in the expression of foreign genes in Pichia pastoris. Bio / Technology 11, 905-911.
14. 14. Dodt, J., Schmitz, T., Schäfer, T., and Bergmann, C. (1986) Expression, secretion and processing of hirudin in E. coli using the alkaline phosphatase signal sequence. FEBS Lett. 202, 373-377.
15. 15. Braun, P. J., Dennis, S., Hofsteenge, J., and Stone, S. R. (1988) Use of site directed mutagenesis to investigate the basis for the specificity of hirudin. Biochemistry 27, 6517-6522.
16. 16. Lazar, J. B., Winant, R. C., and Johnson, P. H. (1991) Hirudin: Amino-terminal residues play a major role in the interaction with thrombin. J. Biol. Chem. 266, 685-688.
17. 17. Riehl-Bellon, N., Carvallo, D., Acker, M., Van Dorsselaer, A., Marquet, M., Loison, G., Lemoine, Y., Brown, S. W., Courtney, M., and Roitsch, C. (1989) Purification and biochemical characterization of recombinant hirudin produced by Saccharomyces cerevisiae. Biochemistry 28, 2941-2949.
18. 18. Achstetter, T., Ngyen-Juilleret, M., Findeli, A., Merkmann, M., and Lemoine, Y. (1992) A new signal peptide useful for secretion of heterologous proteins from yeast and its applications for synthesis of hirudin. Gene 110, 25-31.
19. 19. Weydemann, U., Keup, P., Piontek, M., Strasser, A. W. M., Schweden, J., Gellissen, G., and Janowicz, Z. A. (1995) High-level secretion of hirudin by Hansenula polymorpha-authentic processing of three different preprohirudins. Appl. Microbiol. Biotechnol. 44, 377-385.
20. 20. Bender, E., Vogel, R., Koller, K. P., and Engels, J. (1990) Synthesis and secretion of hirudin by Streptomyces lividans. Appl. Microbiol Biotechnol 34, 203-207.
21. 21. Rade, J. J., Schulick, A. H., Virmani, R., and Dichek, D. A. (1996) Local adenoviral-mediated expression of recombinant hirudin reduces neointima formation after arterial injury. Nature Med. 2, 293-298.
22. 22. Parmenter, D. L., Boothe, J. G., Vanrooijen, G. J. H., Yeung, E. C., and Moloney, M. M. (1995) Production of biologically active hirudin in plant seeds. Plant Mol. Biol. 29, 1167-1180.
23. 23. Stone, S. R., and Maraganore, M. (1993) Hirudin and hirudin-based peptides. Methods Enzymol. 223, 312-336.
24. 24. Laroche, Y., Storme, V., De Meutter, J., Messens, J., and Lauwereys, M. (1994) High level secretion and very efficient isotopic labeling of tick anticoagulant peptide (TAP) expressed in the methylotrophic yeast, Pichia pastoris. Bio / Technology 12,1119-1124.
25. 25. Brankamp, R. G., Sreekrishna, K, Smith, P. L., Blankenship, D. T., and Cardin, A. D. (1995) Expression of a synthetic gene encoding the anticoagulant-antimetastatic protein ghilanten by the methylotropic yeast, Pichia pastoris. Protein Express. Purif. 6, 813-820.