Expression of trimeric CD40 ligand in Pichia pastoris: Use of a rapid method to detect high-level expressing transformants

Gene

Volumn 187, Issue 2, 1997, Pages 193-200

  McGrew, Jeffrey T ^a   Leiske, Dan ^a   Dell, Brad ^a   Klinke, Ralph ^a   Krasts, Dace ^a   Wee, SiowFong ^a   Abbott, Nick ^a   Armitage, Richard ^a   Harrington, Kimberly ^a  

^a AMGEN INC   (United States)

Author keywords

Immunological screen; Leucine zipper trimer; Mixed feed fermentation; Protein production

Indexed keywords

GLYCEROL; GLYCOPROTEIN GP 39; METHANOL;

ARTICLE; B LYMPHOCYTE; CELL DENSITY; ENDOMYCETALES; EXPRESSION VECTOR; FERMENTATION; GENETIC TRANSFORMATION; HUMAN; HUMAN CELL; NONHUMAN; PRIORITY JOURNAL; YEAST;

AMINO ACID SEQUENCE; ANIMALS; CD40 LIGAND; CLONING, MOLECULAR; FERMENTATION; GENE EXPRESSION; GENETIC VECTORS; HUMANS; MEMBRANE GLYCOPROTEINS; MICE; MOLECULAR SEQUENCE DATA; PICHIA; RECOMBINANT FUSION PROTEINS;

PICHIA; PICHIA PASTORIS; SACCHAROMYCETALES;

EID: 0030974549     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(96)00747-0     Document Type: Article

References (18)

1. Armitage, R.J., Fanslow, W.C., Strockbine, L., Sato, T.A., Clifford, K.N., Macduff, B.M., Anderson, D.M., Gimpel, S.D., Davis-Smith, T., Maliszewski, C.R. and et al.: Molecular and biological characterization of a murine ligand for CD40. Nature 357 (1992) 80-82.
2. Armitage, R.J., Macduff, B.M., Eisenman, J., Paxton, R. and Grabstein, K.H. (1995) IL-15 has stimulatory activity for the induction of B cell proliferation and differentiation. J. Immunol. 154, 483-490.
3. Brierley, R.A., Bussineau, C., Kosson, R., Melton, A. and Siegel, R.S. (1990) Fermentation development of recombinant Pichia pastoris expressing the heterologous gene: bovine lysozyme. Ann. N.Y. Acad. Sci. 589, 350-362.
4. Crane, D.I. and Gould, S.J. (1994) The Pichia pastoris HIS4 gene: nucleotide sequence, creation of a non-reverting his4 deletion mutant, and development of HIS4-based replicating and integrating plasmids. Curr. Genet. 26, 443-450.
5. Cregg, J.M., Barringer, K.J., Hessler, A.Y. and Madden, K.R. (1985) Pichia pastoris as a host system for transformations. Mol. Cell. Biol. 5, 3376-3385.
6. Despreaux, C.W. and Manning, R.F. (1993) The dacA gene of Bacillus stearothermophilus coding for D-alanine carboxypeptidase: cloning, structure and expression in Escherichia coli and Pichia pastoris. Gene 131, 35-41.
7. Fanslow, W.C., Srinivasan, S., Paxton, R., Gibson, M.G., Spriggs, M.K. and Armitage, R.J. (1994) Structural characteristics of CD40 ligand that determine biological function. Semin. Immunol. 6, 267-278.
8. Funakoshi, S., Longo, D.L., Beckwith, M., Conley, D.K., Tsarfaty, G., Tsarfaty, I., Armitage, R.J., Fanslow, W.C., Spriggs, M.K. and Murphy, W.J. (1994) Inhibition of human B-cell lymphoma growth by CD40 stimulation. Blood 83, 2787-2794.
9. Harbury, P.B., Zhang, T., Kim, P.S. and Alber, T. (1993) A switch between two-, three-, and four-stranded coiled coils in GCN4 leucine zipper mutants. Science 262, 1401-1407.
10. Holm, C., Meeks-Wagner, D.W., Fangman, W.L. and Botstein, D. (1986) A rapid, efficient method for isolating DNA from yeast. Gene 42, 169-173.
11. Lambie, E.J. and Roeder, G.S. (1986) Repression of meiotic crossing over by a centromere (CEN3) in Saccharomyces cerevisiae. Genetics 114, 769-789.
12. Laroche, Y., Storme, V., De Meutter, J., Messens, J. and Lauwereys, M. (1994) High-level secretion and very efficient isotopic labeling of tick anticoagulant peptide (TAP) expressed in the methylotrophic yeast, Pichia pastoris. Biotechnology 12, 1119-1124.
13. Peitsch, M.C. and Jongeneel, C.V. (1993) A 3-D model for the CD40 ligand predicts that it is a compact trimer similar to the tumor necrosis factors. Int. Immunol. 5, 233-238.
14. Renshaw, B.R., Fanslow, W.C., Armitage, R.J., Campbell, K.A., Liggitt, D., Wright, B., Davison, B.L. and Maliszewski, C.R. (1994) Humoral immune responses in CD40 ligand-deficient mice. J. Exp. Med. 180, 1889-1900.
15. Romanos, M. (1995) Advances in the use of Pichia pastoris for high-level gene expression. Curr. Opin. Biotechnol. 6, 527-533.
16. Scorer, C.A., Clare, J.J., McCombie, W.R., Romanos, M.A. and Sreekrishna, K. (1994) Rapid selection using G418 of high copy number transformants of Pichia pastoris for high-level foreign gene expression. Biotechnology 12, 181-184.
17. Spriggs, M.K., Armitage, R.J., Strockbine, L., Clifford, K.N., Macduff, B.M., Sato, T.A., Maliszewski, C.R. and Fanslow, W.C. (1992) Recombinant human CD40 ligand stimulates B cell proliferation and immunoglobulin E secretion. J. Exp. Med. 176, 1543-1550.
18. Thill, G.P., Davis, G.R., Stillman, C., Holtz, G., Brierley, R.A., Engel, M., Buckholz, R., Kenney, J., Provow, S., Vedick, T. and Siegel, R.S. (1990) Positive and negative effects of multi-copy integrated expression vectors on protein expression in Pichia pastoris. Societé Française de Microbiologie, Paris.