Inducible expression of a heterologous protein in Hansenula polymorpha using the alcohol oxidase 1 promoter of Pichia pastoris

Gene

Volumn 177, Issue 1-2, 1996, Pages 163-167

  Raschke, William C ^a,c   Neiditch, Barry R ^a   Hendricks, Michelle ^a   Cregg, James M ^b  

^a SALK INSTITUTE FOR BIOLOGICAL STUDIES   (United States)

^b OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

^c SIDNEY KIMMEL CANCER CENTER   (United States)

Author keywords

Heterologous gene expression; Methylotrophic yeast; MOX promoter; Transcriptional regulation

Indexed keywords

ALCOHOL OXIDASE; METHANOL;

ARTICLE; CONTROLLED STUDY; ENDOMYCETALES; FUNGUS GROWTH; GENE CASSETTE; GENE EXPRESSION REGULATION; GENETIC TRANSCRIPTION; HANSENULA POLYMORPHA; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; TRANSCRIPTION REGULATION; YEAST;

FUNGI; PICHIA ANGUSTA; PICHIA PASTORIS; SACCHAROMYCETALES;

EID: 0030600453     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-1119(96)00293-4     Document Type: Article

References (23)

1. Ahmad, I., Finkelstein, J. and Steggles, A. (1990) The analysis of RNA by in situ agarose gel hybridization is more sensitive than the equivalent Northern blot analysis. Biotechniques 8, 162.
2. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. and Struhl, K. (Eds.) (1994) Current Protocols in Molecular Biology. John Wiley and Sons, New York.
3. Barberis, A., Superti-Furga, G. and Busslinger, M. (1987) Mutually exclusive interaction of the CCATT-binding factor and of a displacement protein with overlapping sequences of a histone gene promoter. Cell 50, 347-359.
4. Broach, J.R., Strathern, J.N. and Hicks, J.B. (1979) Transformation of yeast: development of a hybrid cloning vector and isolation of the CAN1 gene. Gene 8, 121-133.
5. Buckholz, R.G. and Gleeson, M.A.G. (1991) Yeast systems for the commercial production of heterologous proteins. Biotechnology 9, 1067-1072.
6. Couderc, R. and Baratti, J. (1980) Oxidation of methanol by the yeast Pichia pastoris: purification and properties of alcohol oxidase. Agric. Biol. Chem. 44, 2279-2289.
7. Cregg, J.M. and Madden, K.R. (1988) Development of the methylotrophic yeast, Pichia pastoris, as a host system for the production of foreign proteins. Dev. Ind. Microbiol. 29, 33-41.
8. Cregg, J.M., Barringer, K.J., Hessler, A.Y. and Madden, K.R. (1985) Pichia pastoris as a host system for transformations. Mol. Cell. Biol. 5, 3376-3385.
9. Cregg, J.M., Vedvick, T.S. and Raschke, W.C. (1993) Recent advances in the expression of foreign genes in Pichia pastoris. Biotechnology 11, 905-910.
10. Egli, T., Van Dijken, J.P., Veenhuis, M., Harder, W. and Feichter, A. (1980) Methanol metabolism in yeasts: regulation of the synthesis of catabolic enzymes. Arch. Microbiol. 124, 115-121.
11. Elble, R. (1992) A simple and efficient procedure for transformation of yeasts. Biotechniques 13, 18-20.
12. Gellissen, G., Weydemann, U., Strasser, A.W.M., Piontek, M., Janowicz, Z.A. and Hollenberg, C.P. (1992) Progress in developing methylotrophic yeasts as expression systems. Trends Biotechnol. 10, 413-417.
13. Gödecke, S., Eckart, M., Janowicz, Z.A. and Hollenberg, C.P. (1994) Identification of sequences responsible for transcriptional regulation of the strongly expressed methanol oxidase-encoding gene in Hansenula polymorpha. Gene 139, 35-42.
14. Klebe, R.J., Harriss, J.V., Sharp, Z.D. and Douglas, M.G. (1983) A general method for polyethylene-glycol-induced genetic transformation of bacteria and yeast. Gene 25, 333-341.
15. Koutz, P., Davis, G.R., Stillman, C, Barringer, K., Cregg, J. and Thill, G. (1989) Structural comparison of the Pichia pastoris alcohol oxidase genes. Yeast 5, 161-177.
16. Ledeboer, A.M., Edens, E., Maat, F., Visser, C., Bos, J.W., Verrips, C.T., Janowicz, Z., Eckart, M., Roggenkamp, R. and Hollenberg, C.P. (1985) Molecular cloning and characterization of a gene coding for methanol oxidase in Hansenula polymorpha. Nucleic Acids Res. 13, 3063-3082.
17. Ohi, H., Miura, M., Hiramatsu, R. and Ohumura, T. (1994) The positive and negative cis-acting elements for methanol regulation in the Pichia pastoris AOX2 gene. Mol. Gen. Genet. 243, 489-499.
18. Romanos, M.A., Scorer, C.A. and Clare, J.J. (1992) Foreign gene expression in yeast: a review. Yeast 8, 423-488.
19. Skalnik, D.G., Strauss, E.C. and Orkin, S.H. (1991) CCAAT displacement protein as a represser of the myelomonocytic-specific gr91-phox gene promoter. J. Biol. Chem. 25, 16736-16744.
20. Tschopp, J.F., Brust, P.F., Cregg, J.M., Stillman, C.A. and Gingeras, T.R. (1987) Expression of the lacZ gene from two methanol-regulated promoters in Pichia pastoris. Nucleic Acids Res. 15, 3859-3876.
21. Veale, R.A., Guiseppin, M.L.F., Van Eijk, H.M.J., Sudbery, P.E. and Verrips, C.T. (1992) Development of a strain of Hansenula polymorpha for the efficient expression of Guar α-galactosidase. Yeast 8, 361-372.
22. Veenhuis, M., Van Dijken, J.P. and Harder, W. (1983) The significance of peroxisomes in the metabolism of one-carbon compounds. Adv. Microb. Physiol. 24, 1-82.
23. Wagner, S.L., Siegel, R.S., Vedvick, T.S., Raschke, W.C. and Van Nostrand, W.E. (1992) High level expression, purification and characterization of the Kunitz-type protease inhibitor domain of protease nexin-2/amyloid β-protein precursor. Biochem. Biophys. Res. Commun. 186, 1138-1145.