A semi-synthetic repertoire of intrinsically stable antibody fragments derived from a single-framework scaffold

Journal of Molecular Biology

Volumn 310, Issue 3, 2001, Pages 603-615

  Desiderio, Angiola ^a   Franconi, Rosella ^a   Lopez, Marian ^a   Villani, Maria Elena ^a   Viti, Francesca ^b   Chiaraluce, Roberta ^c   Consalvi, Valerio ^c   Neri, Dario ^b   Benvenuto, Eugenio ^a  

^a ENEA C R CASACCIA   (Italy)

^b ETH ZURICH   (Switzerland)

^c SAPIENZA UNIVERSITY OF ROME   (Italy)

Author keywords

Antibody engineering; Disulphide bond; Phage display; Protein stability; scFv fragment

Indexed keywords

ANTIBODY;

AMINO ACID SEQUENCE; ANTIBODY PRODUCTION; ANTIGEN BINDING; ARTICLE; BINDING AFFINITY; BINDING SITE; ESCHERICHIA COLI; IMMUNOCHEMISTRY; MOLECULAR CLONING; NONHUMAN; PHAGE DISPLAY; PRIORITY JOURNAL; PROTEIN STABILITY; SYNTHESIS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0035854543     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2001.4756     Document Type: Article

References (56)

1. Libraries of peptides and proteins displayed on filamentous phages
2. Phage antibodies: Filamentous phage displaying antibody variable domains
3. Man-made antibodies
4. Multi-subunit proteins on the surface of filamentous phage: Methodologies for displaying antibody (Fab) heavy and light chains
5. By-passing immunization. Human antibodies from V-gene libraries displayed on phage
6. Making antibodies by phage display technology
7. Single-chain antigen-binding protein
8. Protein engineering of antibody binding sites: Recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli
9. Phage-display technology; finding a needle in a vast molecular haystack
10. Natural and designer binding sites made by phage display technology
11. Antibody fragments from a "single pot" phage display library as immunochemical reagents
12. Isolation of high affinity human antibodies directly from large synthetic repertoires
13. Human antibodies with sub-nanomolar affinities isolated from a large non-immunized phage display library
14. Efficient construction of a large non-immune phage antibody library: The production of high-affinity human single-chain antibodies to protein antigens
15. A large non-immunized human Fab fragment phage library that permits rapid isolation and kinetic analysis of high affinity antibodies
16. Fully synthetic human combinatorial antibody libraries (HuCAL) based on modular consensus frameworks and CDRs randomized with trinucleotides
17. Ribosome display efficently selects and evolves high-affinity antibodies in vitro from immune libraries
18. Improving antibody affinity by mimicking somatic hypermutation in vitro
19. Antibody scFv fragments without disulphide bonds made by molecular evolution
20. Selection for improved protein stability by phage display
21. Intracellular antibodies (intrabodies) for gene therapy of infectious diseases
22. The selection of intracellular antibodies
23. Transgenic plants expressing a functional single-chain Fv antibody are specifically protected from virus attack
24. A single-chain antibody ragment is functionally expressed in the cytoplasm of both Escherichia coli and transgenic plants
25. A mutational analysis of the binding of two different proteins to the same antibody
26. Unfolding free energy changes determined by the linear extrapolation method. 1. Unfolding of phenylmethane-sulfonyl alpha-chymotrypsin using different denaturants
27. Redox state of single-chain Fv fragments targeted to the endoplasmic reticulum, cytosol and mitochondria
28. H: Consequences for thermodynamic stability and folding
29. An intrinsically stable antibody scFv fragment can tolerate the loss of both disulphide bonds and fold correctly
30. Different equilibrium stability behaviour of scFv fragments: Identification, classification, and improvement by protein engineering
31. Expression of an antibody fragment at high levels in the bacterial cytoplasm
32. Improving in vivo folding and stability of a single-chain Fv antibody fragment by loop grafting
33. Conformations of immunoglobulin hypervariable regions
34. Anatomy of the antibody molecule
35. Standard conformations for the canonical structures of immunoglobulins
36. Automated classification of antibody complementarity region 3 of the heavy chain (H3) loops into canonical forms and its application to protein structure prediction
37. H domain of immunoglobulins
38. Domain association in immunoglobulin molecules. The packing of variable domains
39. Canonical structures for the hypervariable regions of immunoglobulins
40. Design and use of a phage display library. Human antibodies with subnanomolar affinity against a marker of angiogenesis eluted from a two-dimensional gel
41. H molecules with a unique interface
42. H gene segments rearranged in vitro
43. Recombining germline-derived CDR sequences for creating diverse single-framework antibody libraries
44. In vitro folding and thermodynamic stability of an antibody fragment selected in vivo for high expression levels in Escherichia coli cytoplasm
45. Folding and association versus misfolding and aggregation of proteins
46. Selection of antibodies for intracellular function using a two-hybrid in vivo system
47. Analysis of the interaction between single-chain variable fragments and their antigen in a reducing intracellular environment using the two-hybrid system
48. In vivo selection of single-chain antibodies using a yeast two-hybrid system
49. Plantibodies: Immunomodulation and immunotherapeutic potential
50. The plantibody approach: Expression of antibody genes in plants to modulate plant metabolism or to obtain pathogen resistance
51. 32P]-ATP
52. The expression of "single chain" antibodies in transgenic plants
53. Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins
54. Resolution of the fluorescence equilibrium unfolding profile of trp aporepressor using single tryptophan mutants
55. Cleavage of structural proteins during the assembly of the head of bacteriophage T4