In vivo selection of single-chain antibodies using a yeast two-hybrid system

Journal of Immunological Methods

Volumn 238, Issue 1-2, 2000, Pages 161-172

  Pörtner Taliana, Antje ^a   Russell, Marijane ^a   Froning, Karen J ^a   Budworth, Paul R ^b   Comiskey, John D ^a   Hoeffler, James P ^c  

^a INVITROGEN CORPORATION   (United States)

^b Novartis Ag Discovery Institute   (United States)

^c Active Motif   (United States)

Author keywords

Library screen; Single chain antibody; Transcription factor; Yeast two hybrid system

Indexed keywords

TRANSCRIPTION FACTOR;

ANTIGEN DETECTION; ARTICLE; GENE CONSTRUCT; GENE EXPRESSION; HYBRID CELL; PLASMID; PRIORITY JOURNAL; TECHNIQUE; WESTERN BLOTTING; YEAST;

ACTIVATING TRANSCRIPTION FACTOR 2; ANIMALS; ANTIBODY SPECIFICITY; CHO CELLS; COS CELLS; CRICETINAE; CYCLIC AMP RESPONSE ELEMENT-BINDING PROTEIN; HUMANS; IMMUNOGLOBULIN FRAGMENTS; IMMUNOGLOBULIN VARIABLE REGION; SACCHAROMYCES CEREVISIAE; TRANSCRIPTION FACTORS; TWO-HYBRID SYSTEM TECHNIQUES;

BACTERIA (MICROORGANISMS); MAMMALIA;

EID: 0343527530     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(00)00145-9     Document Type: Article

References (23)

1. Anborgh P.H., Qian X., Papageorge A.G., Vass W.C., DeClue J.E., Lowy D.R. Ras-specific exchange factor GRF: oligomerization through its Dbl homology domain and calcium-dependent activation of Raf. Mol. Cell. Biol. 19:1999;4611.
2. Cattaneo A., Biocca S. Intracellular Antibodies: Development and Applications. 1997;Springer, New York.
3. Chien C.T., Bartel P.L., Sternglanz R., Fields S. The two-hybrid system: a method to identify and clone genes for proteins that interact with a protein of interest. Proc. Natl. Acad. Sci. USA. 88:1991;9578.
4. Cochet O., Kenigsberg M., Deluneau I., Virone-Oddos A., Multonm M.C., Fridman W.H., Schweighofer F., Teillaud J.L., Tocque B. Intracellular expression of an antibody fragment-neutralizing p21 Ras promotes tumor regression. Cancer Res. 58:1998;1170.
5. Coloma M.J., Larrick J.W., Ayala M., Gavilondo-Cowley J.V. Primer design for the cloning for the immunoglobulin heavy-chain leader-variable regions from mouse hybridoma cells. BioTechniques. 11:1991;152.
6. Coloma M.J., Hastings A., Wims L.A., Morrison S.L. Novel vectors for the expression of antibody molecules using variable regions generated by polymerase chain reaction. J. Immunol. Methods. 152:1992;89.
7. Evan G.I., Lewis G.K., Ramsay G., Bishop V.M. Isolation of monoclonal antibodies specific for human c-myc protooncogen product. Mol. Cell Biol. 5:1985;3610.
8. Fields S., Song O. A novel genetic system to detect protein-protein interaction. Nature. 340:1989;245.
9. Gyuris J., Golemis E., Chertkov H., Brent R. Cdi1, a human G1 and S phase protein phosphatase that associates with Cdk2. Cell. 75:1993;791.
10. Hoogenboom H.R., Griffiths A.D., Johnson K.S., Chiswell D.J., Hudson P., Winter G. Multisubunit proteins on the surface of filamentous phage methodologies for displaying antibody (Fab) heavy and light chains. Nucleic Acids Res. 19:1991;4133.
11. Hwang C., Sinskey A.J., Lodish H.F. Oxidized redox state of glutathione in the endoplasmic reticulum. Science. 257:1992;1496.
12. Kroll D.J., Abdel-Malek Abdel Hafiz H., Marcell T., Simpson S., Chen C.Y., Gutierrez-Hartmann A., Lustbader J.W., Hoeffler J.P. A multifunctional protein expression system: overproduction, affinity purification, and selective detection. DNA Cell Biol. 12:1993;441.
13. Maekawa T., Sakura H., Kanei-Ishii C., Sudo T., Yoshimura T., Fujisawa J., Yoshida M., Ishii S. Leucine zipper structure of the protein CRE-BP1 binding to the cAMP responsive element in brain. EMBO J. 8:1989;2023.
14. Marks J.D., Hoogenboom H.R., Bonnert T.P., McCafferty J., Griffiths A.D., Winter G. By-passing immunization. Human antibodies from V-gene libraries displayed on phage. J. Mol. Biol. 222:1991;581.
15. Martineau P., Jones P., Winter G. Expression of an antibody fragment at high levels in the bacterial cytoplasm. J. Mol. Biol. 280:1998;117.
16. Nicholls P.J., Johnson V.G., Blanford M.D., Andrew S.M. An improved method for generating single-chain antibodies from hybridomas. Immunol. Methods. 165:1993;81.
17. Proba K., Worn A., Honegger A., Plückthuhn A. Antibody scFv fragments without disulfide bonds made by molecular evolution. J. Mol. Biol. 275:1998;245.
18. Quinn P. Distinct activation domains within cAMP responsive element-binding protein (CREB) mediate basal and cAMP stimulated transcription. J. Biol. Chem. 268:1993;16999.
19. Richardson J.H., Sodroski J.G., Waldmann T.A., Maresco W.A. Phenotypic knockout of the high-affinity human interleukin 2 receptor by intracellular single-chain antibodies against the α subunit of the receptor. Proc. Natl. Acad. Sci. USA. 92:1995;3137.
20. Rondon I.J., Marasco W.A. Intracellular antibodies (intrabodies) for gene therapy of infectious diseases. Annu. Rev. Microbiol. 51:1997;2567.
21. Schiestl R.H., Geitz R.D. High efficiency transformation of intact yeast cells using single stranded nucleic acids as carrier. Curr. Genet. 16:1989;339.
22. Vojtek A.B., Hollenberg S.M., Cooper J.A. Mammalian Ras interacts directly with serine threonine kinase RAF. Cell. 74:1993;205.
23. Ward A.C. Single-step purification of shuttle vectors from yeast or high frequency back transformation into E. coli. Nucleic Acids Res. 18:1990;5319.