The plantibody approach: Expression of antibody genes in plants to modulate plant metabolism or to obtain pathogen resistance

Plant Molecular Biology

Volumn 43, Issue 4, 2000, Pages 419-428

  De Jaeger, G ^a   De Wilde, C ^a   Eeckhout, D ^a   Fiers, E ^a   Depicker, A ^a  

^a GHENT UNIVERSITY   (Belgium)

Author keywords

Antibody production; Gene inactivation; Immunomodulation; Phage display; Plantibody; Reducing environment; Single chain variable fragment

Indexed keywords

CELL METABOLISM; DISEASE RESISTANCE; GENETIC REGULATION; IMMUNOMODULATION; PATHOGEN; PEST RESISTANCE; PHYTOHORMONE; PLANTIBODY;

ANTIBODIES; GENE EXPRESSION REGULATION, PLANT; PLANT DISEASES; PLANTS; PLANTS, GENETICALLY MODIFIED; SUPPORT, NON-U.S. GOV'T;

EID: 0033810842     PISSN: 01674412     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1006471528756     Document Type: Short Survey

References (44)

1. Expression of a single-chain Fv antibody against abscisic acid creates a wilty phenotype in transgenic tobacco
2. Intracellular immunomodulation in plants - A new tool for the investigation of phytohormones
3. Expression in tobacco of a functional monoclonal antibody specific to stylet secretions of the root-knot nematode
4. The fate of immunoglobulin G fed to larvae of Ostrinia nubilalis
5. Antisense strategies for genetic manipulations in plants
6. Expression of engineered antibodies in plants: A possible tool for spiroplasma and phytoplasma disease control
7. A large non-immunized human Fab fragment phage library that permits rapid isolation and kinetic analysis of high affinity antibodies
8. Use of phage display for isolation and characterization of single-chain variable fragments against dihydroflavonol 4-reductase from Petunia hybrida
9. High level accumulation of single-chain variable fragments in the cytosol of transgenic Petunia hybrida
10. Analysis of the interaction between single-chain variable fragments and their antigen in a reducing intracellular environment using the two-hybrid system
11. Transient expression of IgG antibodies and antibody fragments in intact leaf tissue of Nicotiana tabacum
12. Production of antibodies in transgenic plants - A general introduction
13. Lucilla cuprina: Inhibition of larval growth induced by immunization of host sheep with extracts of larval peritrophic membrane
14. Engineering of Beet necrotic yellow vein virus (BNYVV) resistance in Nicotiana benthamiana
15. Nicotiana benthamiana plants expressing beet necrotic yellow vein virus (BNYVV) coat protein-specific scFv are partially protected against the establishment of the virus in the early stages of infection and its pathogenic effects in the late stages of infection
16. Molecular farming of recombinant antibodies in plants
17. Isolation of high affinity human antibodies directly from large synthetic repertoires
18. Naturally occurring antibodies devoid of light chains
19. In vitro selection and evolution of functional proteins by using ribosome display
20. Genetic control of dihydroflavonol 4-reductase gene expression in Petunia hybrida
21. An Agrobacterium-mediated transient gene expression system for intact leaves
22. Potent enzyme inhibitors derived from dromedary heavy-chain antibodies
23. Engineering of a single-chain variable-fragment (scFv) antibody specific for the stolbur phytoplasma (mollicute) and its expression in Escherichia coli and tobacco plants
24. Digestion and fate of the vertebrate blood-meal in insects
25. Diverting a protein from its cellular location by intracellular antibodies. The case of p21Ras
26. Intracellular antibodies (intrabodies) as research reagents and therapeutic molecules for gene therapy
27. Synthesis of a functional anti-phytochrome single-chain F(υ) protein in transgenic tobacco
28. Seed-specific immunomodulation of abscisic acid activity induces a developmental switch
29. Expression and functional characterization of a single chain Fv antibody directed against secretions involved in plant nematode infection process
30. Exploiting recombination in single bacteria to make large phage antibody libraries
31. Plantibodies - Requirements for expression and subcellular targeting
32. The C-terminal KDEL sequence increases the expression level of a single chain antibody designed to be targeted to both the cytosol and the secretory pathway in transgenic tobacco
33. Improving scFv antibody expression levels in the plant cytosol
34. Efficient construction of a large nonimmune phage antibody library: The production of high-affinity human single-chain antibodies to protein antigens
35. Expression of a functional single-chain antibody against GA(24/19) in transgenic tobacco
36. Endogenous cellulases in animals: Isolation of β-1,4-endoglucanase genes from two species of plant-parasitic cyst nematodes
37. Transgenic plants expressing a functional single-chain Fv antibody are specifically protected from virus attack
38. A single-chain antibody fragment is functionally expressed in the cytoplasm of both Excherichia coli and transgenic plants
39. Human antibodies with sub-nanomolar affinities isolated from a large non-immunized phage display library
40. Reduced virus infectivity in N. tabacum secreting a TMV-specific full-size antibody
41. Pectate lyase of Colletotrichum gloeosporioides attacking avocado fruits: cDNA Cloning and involvement in pathogenicity
42. Immunity in vaccinated cattle exposed to experimental and natural infestations with Boophilus microplus
43. Intracellular expression of TMV-specific single-chain Fv fragments leads to improved vires resistance in Nicotiana tabacum